公开(公告)号：EP1598367A1

公开(公告)日：2005-11-23

申请号：EP04011736.8

申请日：2004-05-18

申请人： ZLB Behring GmbH

发明人： Weimer, Thomas, Dr. ,  Schulte, Stefan, Dr. ,  Hofmann, Kay, Dr. ,  Hauser, Hans-Peter, Dr.

IPC分类号： C07K14/745 ,  C07K14/755 ,  C12N15/12

CPC分类号： C07K14/811 ,  C07K14/755 ,  C12N9/0091 ,  C12Y116/03001

摘要： The present invention relates to modified nucleic acid sequences coding for coagulation Factors, in particular human FV and human Factor VIII and their derivatives with improved stability, recombinant expression vectors containing such nucleic acid sequences, host cells transformed with such recombinant expression vectors, recombinant polypeptides and derivatives which do have biological activities of the unmodified wild type protein but having improved stability and processes for the manufacture of such recombinant proteins and their derivatives. The invention also covers a transfer vector for use in human gene therapy, which comprises modified DNA sequences.

摘要翻译： 本发明涉及编码凝血因子的修饰的核酸序列，特别是人FV和人因子VIII及其衍生物具有改善的稳定性，含有此类核酸序列的重组表达载体，用这种重组表达载体转化的宿主细胞，重组多肽和 确实具有未修饰的野生型蛋白质的生物活性但具有改进的稳定性的生物活性衍生物和用于制备这些重组蛋白质及其衍生物的方法。 本发明还涵盖用于人基因治疗的转移载体，其包含修饰的DNA序列。