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    DIGITAL MEASUREMENTS FROM TARGETED SEQUENCING
    3.
    发明公开
    DIGITAL MEASUREMENTS FROM TARGETED SEQUENCING 审中-公开
    数字测量从目标测序

    公开(公告)号：EP3177740A1

    公开(公告)日：2017-06-14

    申请号：EP15830393.3

    申请日：2015-08-06

    申请人： Nugen Technologies, Inc.

    发明人： SCOLNICK, Jonathan ,  AMORESE, Douglas ,  HUELGA, Stephanie C. ,  SCHROEDER, Benjamin

    IPC分类号： C12Q1/68 ,  C40B30/04 ,  C40B30/00

    CPC分类号： G06F19/22 ,  C12Q1/6806 ,  C12Q1/6886 ,  C12Q1/689 ,  C12Q2600/158 ,  G06F19/28 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2537/165

    摘要： Disclosed herein are methods, compositions and kits for quantitating one or more specific nucleic acids within a plurality of nucleic acids. In some embodiments, a sequencing library is constructed from enriched probe extension products specific for the specific nucleic acids and sequenced. In some embodiments, the resulting reads are used for removing duplicate reads. In some embodiments, counting of verified probes is used to quantitate or determine the number of specific nucleic acid molecules in the starting nucleic acid sample.

    摘要翻译： 本文公开了用于定量多种核酸内的一种或多种特定核酸的方法，组合物和试剂盒。 在一些实施方案中，测序文库由对特定核酸特异的富集的探针延伸产物构建并测序。 在一些实施例中，所得读取被用于移除重复读取。 在一些实施方案中，使用经验证的探针的计数来定量或确定起始核酸样品中特定核酸分子的数量。

    ISOTHERMAL NUCLEIC ACID AMPLIFICATION METHODS AND COMPOSITIONS
    4.
    发明公开
    ISOTHERMAL NUCLEIC ACID AMPLIFICATION METHODS AND COMPOSITIONS 审中-公开
    方法和组合物的NUCLEIC等温扩增

    公开(公告)号：EP2250283A2

    公开(公告)日：2010-11-17

    申请号：EP09709858.6

    申请日：2009-02-12

    申请人： Nugen Technologies, Inc.

    发明人： KURN, Nurith ,  WANG, Shenglong

    IPC分类号： C12Q1/68 ,  C12N15/11

    CPC分类号： C12Q1/6844 ,  C12Q2565/537 ,  C12Q2525/121 ,  C12Q2521/319 ,  C12Q2531/119

    摘要： Methods and compositions are provided related to the amplification of target polynucleotide sequences as well as total RNA and total DNA amplification. In some embodiments, the methods and compositions also allow for the immobilization and capture of target polynucleotides with defined 3' and or 5' sequences to solid surfaces. The polynucleotides attached to the solid surfaces can be amplified or eluted for downstream processing. In some cases, nucleotides attached to solid surfaces can be used for high throughput sequencing of nucleotide sequences related to target DNA or target RNA.

    METHOD FOR ARCHIVING AND CLONAL EXPANSION
    5.
    发明公开
    METHOD FOR ARCHIVING AND CLONAL EXPANSION 审中-公开
    程序申请，并扩展克隆

    公开(公告)号：EP2247727A2

    公开(公告)日：2010-11-10

    申请号：EP09711405.2

    申请日：2009-02-12

    申请人： Nugen Technologies, Inc.

    发明人： KURN, Nurith

    IPC分类号： C12N15/10

    CPC分类号： C12Q1/6844 ,  C12Q2565/537 ,  C12Q2525/121 ,  C12Q2521/319 ,  C12Q2531/119

    摘要： The present method provides methods, libraries, and kits related to the archiving and clonal expansion of sequences related to target polynucleotide sequences. The method allow for the attachment of polynucleotides with defined 3' and or 5' sequences to solid surfaces. The polynucleotides attached to the solid substrates can be stored or archived as libraries and can subsequently be retrieved for analysis, for example by clonal expansion. In some embodiments, nucleotides attached to solid surfaces can be used for sequencing of nucleotide sequences related to target RNA or target RNA. The methods are applicable to total RNA and/or total DNA analysis.

    METHODS AND COMPOSITIONS FOR AMPLIFICATION OF RNA SEQUENCES
    8.
    发明授权
    METHODS AND COMPOSITIONS FOR AMPLIFICATION OF RNA SEQUENCES 有权
    方法和组合物繁殖的RNA序列

    公开(公告)号：EP1366197B1

    公开(公告)日：2007-05-09

    申请号：EP02721342.0

    申请日：2002-03-11

    申请人： Nugen Technologies, Inc.

    发明人： KURN, Nurith

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6853 ,  C12Q2531/119

    摘要： The invention provides methods for isothermal amplification of RNA. The methods are particularly suitable for amplifying a plurality of RNA species in a sample. The methods employ a composite primer, a second primer and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In another aspect, the methods employ a single primer (which is a composite primer) and strand displacement to generate multiple copies of DNA products comprising sequences complementary to an RNA sequence of interest. In some embodiments, a transcription step is included to generate multiple copies of sense RNA of an RNA sequence of interest. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.

    COMPOSITIONS AND METHODS FOR TARGETED NUCLEIC ACID SEQUENCE ENRICHMENT AND HIGH EFFICIENCY LIBRARY GENERATION
    10.
    发明公开
    COMPOSITIONS AND METHODS FOR TARGETED NUCLEIC ACID SEQUENCE ENRICHMENT AND HIGH EFFICIENCY LIBRARY GENERATION 审中-公开
    靶向核酸序列富集和高效率文库生产的组合物和方法

    公开(公告)号：EP3186418A2

    公开(公告)日：2017-07-05

    申请号：EP15836587.4

    申请日：2015-08-26

    申请人： Nugen Technologies, Inc.

    发明人： AMORESE, Doug ,  KURN, Nurith ,  SCOLNICK, Jonathan ,  WANG, Alexandra, Hui

    IPC分类号： C40B40/06 ,  C40B50/06 ,  C12P19/34

    CPC分类号： C12N15/1096 ,  C12N15/1093

    摘要： Provided herein are methods, compositions and kits for targeted nucleic acid sequence enrichment in a nucleic acid sample and for high efficiency nucleic acid library generation for next generation sequencing (NGS). The methods, compositions and kits provided herein can be useful for the production and capture of amplification-ready, target-specific and strand-specific regions of interest from nucleic acid samples containing complex DNA.

    摘要翻译： 本文提供了用于靶核酸序列在核酸样品中富集和用于下一代测序（NGS）的高效核酸文库生成的方法，组合物和试剂盒。 本文提供的方法，组合物和试剂盒可用于从含有复合DNA的核酸样品生产和捕获扩增就绪，靶特异性和链特异性感兴趣区域。