公开(公告)号：EP2510107A2

公开(公告)日：2012-10-17

申请号：EP10836574.3

申请日：2010-12-07

申请人： Synaptic Research LLC

发明人： OYLER, George A. ,  CHANG, Yung-Nien ,  TSAI, Yien Che

IPC分类号： C12Q1/37 ,  C12N15/09 ,  C12Q1/68 ,  C12N15/57 ,  C12N15/63

CPC分类号： C12Q1/37 ,  C12N15/1034 ,  C12N15/1086 ,  C12N15/635 ,  C12Q1/6897 ,  C12Q2522/101 ,  C12Q2521/537

摘要： A system for the identification of proteases and protease inhibitors is provided. The system has at least two components. The first component is a reporter construct with at least one binding site, a transcriptional promoter, an inducible promoter region, and at least one reporter gene, all functionally connected for expression of the reporter gene(s) in functional coordination with a transcriptional activation agent. The second component is a transcriptional activation agent comprising a nucleic acid binding domain, at least one protease substrate domain, and at least one transcriptional activation domain for an inducible promoter. The system allows detection and evaluation of agents affecting protease activity directed to the protease substrate domain. The system also allows for the detection of the presence of proteases in environmental samples.

公开(公告)号：EP2470664A2

公开(公告)日：2012-07-04

申请号：EP10815904.7

申请日：2010-08-27

申请人： Synaptic Research LLC

发明人： CHANG, Yung-Nien ,  OYLER, George A.

IPC分类号： C12N15/85 ,  C12N15/31 ,  C12N5/10 ,  C12N5/071 ,  C12R1/145

CPC分类号： C12N5/0696 ,  A61K35/74 ,  A61K38/164 ,  C07K14/33 ,  C07K14/4702 ,  C07K2319/00 ,  C07K2319/10 ,  C07K2319/55 ,  C12N5/00 ,  C12N2501/602 ,  C12N2501/603

摘要： A novel protein delivery system to generate induced pluripotent stem (iPS) cells is described. The delivery system comprises a construct with a receptor binding domain that recognizes a receptor in a somatic cell, a translocation domain that allows the transfer of an inducer into the cytosolic space, and a cargo bearing domain to which the inducer is attached and facilitates transfer of the inducer into the cell.

公开(公告)号：EP2771453A1

公开(公告)日：2014-09-03

申请号：EP12844478.3

申请日：2012-10-26

申请人： The Regents of the University of California ,  Synaptic Research LLC

发明人： DAVIS, Aubrey ,  SCHRIER, Rachel ,  OYLER, George A. ,  ROSENBERG, Julian N.

IPC分类号： C12N1/13 ,  A61K39/106 ,  A61K39/112 ,  C12N15/62

CPC分类号： A61K39/295 ,  A61K36/06 ,  A61K39/00 ,  A61K39/102 ,  A61K39/107 ,  A61K2039/51 ,  A61K2039/517 ,  A61K2039/523 ,  A61K2039/541 ,  A61K2039/542 ,  A61K2039/57 ,  C12N1/12 ,  C12N7/00 ,  C12N15/79 ,  C12N2760/18534 ,  Y02A50/464 ,  Y02A50/472 ,  Y02A50/487

摘要： This invention provides diatom-based vaccines.

公开(公告)号：EP2694543A2

公开(公告)日：2014-02-12

申请号：EP12767799.5

申请日：2012-04-07

申请人： Synaptic Research LLC ,  University of Nebraska Lincoln

发明人： OYLER, George A. ,  ROSENBERG, Julian N ,  WEEKS, Donald P

IPC分类号： C07K16/14 ,  C07K19/00 ,  C12N1/12 ,  C12R1/89

CPC分类号： C07K16/14 ,  C07K2317/22 ,  C07K2317/569 ,  C07K2319/00 ,  C07K2319/20 ,  C07K2319/70 ,  C12N1/02 ,  C12N1/12 ,  C12N1/22

摘要： A single chain antibody that binds algae is described. The single chain antibody for algae is used to capture algae onto bioactive films. The single chain antibody is also used in a chimeric construct having a substrate binding domain and a single chain antibody domain. Dimers, trimmers, and multimer constructs are also described that aid in collection of algae from liquid mixtures by causing flocculation of algae cells.

公开(公告)号：EP2580345A2

公开(公告)日：2013-04-17

申请号：EP11831073.9

申请日：2011-06-13

申请人： Synaptic Research LLC

发明人： OYLER, George A. ,  TSAI, Yien Che

IPC分类号： C12Q1/37 ,  C12N15/63 ,  C12Q1/02 ,  C12N15/57 ,  G01N33/68 ,  C07K14/195

CPC分类号： C12Q1/66 ,  C12Q1/37 ,  G01N2333/33 ,  G01N2333/952 ,  G01N2500/10

摘要： A cell based assay for detection for protease activity is disclosed. In the assay a cell is engineered to express a protease substrate with at least one label, preferably on its C-terminus. Cleavage of the substrate by the protease that recognizes it results in a C-terminal fragment and a N-terminal fragment, where the fragment having the label is subject to ubiquitin proteasome degradation. The assay measures the disappearance of the label due to degradation of the fragment to which it is attached. A cell free assay is also described for detection of protease activity. In the cell free assay, the protease substrate is expressed in a solution that includes the elements of the ubiquitin proteasome pathway for degradation of the fragment. The assay measures the disappearance of the label attached to the fragment that results from cleavage by the protease.

公开(公告)号：EP2494054A2

公开(公告)日：2012-09-05

申请号：EP10827622.1

申请日：2010-11-01

申请人： Synaptic Research LLC

发明人： OYLER, George ,  ROSENBERG, Julian

IPC分类号： C12N15/79 ,  C12N15/62 ,  A01H13/00 ,  C12R1/89

CPC分类号： C12N15/79 ,  C07K14/415 ,  C12N15/8216

摘要： The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.