公开(公告)号：EP2598127B1

公开(公告)日：2017-09-13

申请号：EP11746066.7

申请日：2011-07-28

申请人： Life Technologies Corporation

发明人： AGNEW, Brian ,  SINGH, Upinder ,  GRECIAN, Scott

IPC分类号： A61K31/00 ,  A61K31/19 ,  A61K31/20 ,  C12N7/04

CPC分类号： A61K31/655 ,  A61K31/7008 ,  C12N7/00 ,  C12N2710/14151 ,  C12N2710/14163 ,  C12N2740/16051 ,  C12N2740/16063

摘要： Methods of using azide-modified biomolecules, such as fatty acids, carbohydrates and lipids, to treat a plant or an animal infected with a virus or to inhibit infectivity of a virus, such as the human immunodeficiency virus, are provided. Also provided are methods of labeling a human immunodeficiency virus with an azide-modified biomolecule, such as a fatty acid, a carbohydrate, or an isoprenoid lipid. The azide-modified biomolecules may be combined with a pharmaceutically acceptable excipient to produce a pharmaceutical composition, optionally containing another anti-viral agent and/or a delivery agent, such as a liposome.

摘要翻译： 提供了使用叠氮化物修饰的生物分子如脂肪酸，碳水化合物和脂质来治疗感染病毒的植物或动物或抑制病毒如人类免疫缺陷病毒的感染性的方法。 还提供了用叠氮化物修饰的生物分子如脂肪酸，碳水化合物或类异戊二烯脂质标记人类免疫缺陷病毒的方法。 叠氮化物修饰的生物分子可以与药学上可接受的赋形剂组合以产生药物组合物，任选含有另一种抗病毒剂和/或递送剂，例如脂质体。

公开(公告)号：EP2460819A3

公开(公告)日：2013-01-09

申请号：EP12155381.2

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involve the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP2460818A3

公开(公告)日：2013-01-09

申请号：EP12155379.6

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP2460821A2

公开(公告)日：2012-06-06

申请号：EP12156474.4

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP1833992B1

公开(公告)日：2015-03-04

申请号：EP05856068.1

申请日：2005-12-29

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： EICHMEYER, Marc Allan ,  NITZEL, Greg ,  SCHAEFFER, Merrill Lynn

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

公开(公告)号：EP2281829B1

公开(公告)日：2014-12-17

申请号：EP10174465.4

申请日：2005-12-29

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Eichmeyer, Marc Allan ,  Schaeffer, Merrill Lynn ,  Nitzel, Greg

IPC分类号： C07K14/01 ,  A61K39/12

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

公开(公告)号：EP2460821A3

公开(公告)日：2014-12-10

申请号：EP12156474.4

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP2460820A2

公开(公告)日：2012-06-06

申请号：EP12155382.0

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

摘要翻译： 提供了一种从猪圆环病毒型（2）中回收由开放阅读框（2）表达的蛋白质的改进方法。 该方法通常包括将包含开放阅读框（2）的重组病毒转染到生长培养基中包含的细胞中的步骤，使病毒表达开放阅读框（2），并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约（5）天开始进行，以便允许足够量的重组蛋白从细胞中表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。

公开(公告)号：EP2460816A2

公开(公告)日：2012-06-06

申请号：EP12155376.2

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP2460817B1

公开(公告)日：2017-03-29

申请号：EP12155378.8

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.