公开(公告)号：EP3320919A1

公开(公告)日：2018-05-16

申请号：EP17208901.3

申请日：2006-12-28

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Roof, Michael B. ,  Hayes, Phillip Wayne ,  Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： A61K39/295 ,  A61K39/04 ,  A61K39/12 ,  C12N15/866

摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. Also provided is recombinant PCV2 ORF2 protein, and immunogenic compositions comprising PCV2 ORF2 protein. Moreover, multivalent combination vaccines are provided which include an immunological agent effective for reducing the incidence of or lessening the severity of PCV2 infection, preferably PCV2 ORF2 protein, or an immunogenic composition comprising PCV2 ORF2 protein, and at least one immunogenic active component of another disease-causing organism in swine.

摘要翻译： 提供了从猪圆环病毒2型恢复由开放阅读框2表达的蛋白质的改进方法。 还提供了重组PCV2ORF2蛋白和包含PCV2ORF2蛋白的免疫原性组合物。 此外，提供了包含有效降低PCV2感染发生率或减轻其严重程度的免疫药剂，优选PCV2ORF2蛋白或包含PCV2ORF2蛋白的免疫原性组合物和另一种疾病的至少一种免疫原性活性成分的多价组合疫苗 引起猪的有机体。

公开(公告)号：EP2371385B1

公开(公告)日：2015-08-19

申请号：EP11164854.9

申请日：2006-12-28

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Roof, Michael B. ,  Eichmeyer, Marc Allan ,  Nitzel, Greg ,  Hayes, Phillip Wayne ,  Schaeffer, Merrill Lynn

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

公开(公告)号：EP2374475A1

公开(公告)日：2011-10-12

申请号：EP11164855.6

申请日：2006-12-28

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Roof, Michael B. ,  Hayes, Phillip Wayne ,  Eichmeyer, Marc ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

公开(公告)号：EP2371384A1

公开(公告)日：2011-10-05

申请号：EP11164852.3

申请日：2006-12-28

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Roof, Michael B. ,  Hayes, Phillip Wayne ,  Eichmeyer, Marc ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

公开(公告)号：EP2460817A3

公开(公告)日：2013-01-09

申请号：EP12155378.8

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

摘要翻译： 提供了一种用于从猪圆环病毒2型中回收开放阅读框2表达的蛋白质的改进方法。 该方法通常包括将包含开放阅读框架2编码序列的重组病毒转染到生长培养基中包含的细胞中的步骤，导致病毒表达开放阅读框架2，并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约5天开始，以便允许足够量的重组蛋白从细胞中表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。

公开(公告)号：EP2460819A2

公开(公告)日：2012-06-06

申请号：EP12155381.2

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involve the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

摘要翻译： 提供了从猪圆环病毒类型（2）中回收由开放阅读框（2）表达的蛋白质的改进方法。 该方法一般包括将含有开放阅读框（2）编码序列的重组病毒转染到生长培养基中所含的细胞中，使病毒表达开放阅读框（2），并回收表达的上清中的蛋白质。 这种恢复应该在细胞感染大约（5）天后开始，以便允许足量的重组蛋白质从细胞表达并分泌到生长培养基中。 此类方法避免了从细胞内分离和回收重组蛋白所需的昂贵且耗时的提取程序。

公开(公告)号：EP2460818A2

公开(公告)日：2012-06-06

申请号：EP12155379.6

申请日：2005-12-29

申请人： Boehringer Ingelheim Vetmedica, Inc.

发明人： Eichmeyer, Mark ,  Nitzel, Greg ,  Schaeffer, Merrill

IPC分类号： C07K14/01 ,  A61K39/12 ,  C12N7/04 ,  C12Q1/68 ,  A61K39/295

CPC分类号： A61K33/00 ,  A61K39/12 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/54 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  A61K2039/55566 ,  A61K2039/6081 ,  A61K2039/70 ,  C07K14/005 ,  C12N7/00 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10052 ,  C12N2770/10034 ,  C12N2799/026 ,  C12Q1/701

摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.

公开(公告)号：EP2371385A1

公开(公告)日：2011-10-05

申请号：EP11164854.9

申请日：2006-12-28

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Roof, Michael B. ,  Eichmeyer, Marc Allan ,  Nitzel, Greg ,  Hayes, Phillip Wayne ,  Schaeffer, Merrill Lynn

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

公开(公告)号：EP2371382A1

公开(公告)日：2011-10-05

申请号：EP11164849.9

申请日：2006-12-28

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Roof, Michael B. ,  Eichmeyer, Marc Allan ,  Nitzel, Greg ,  Hayes, Phillip Wayne ,  Schaeffer, Merrill Lynn

IPC分类号： A61K39/12

CPC分类号： A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55516 ,  A61K2039/55555 ,  A61K2039/55566 ,  C12N7/00 ,  C12N2750/10022 ,  C12N2750/10023 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2750/10071 ,  C12N2750/14143

摘要： The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and/or multinucleated/giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

摘要翻译： 本发明涉及使用免疫原性组合物的那样包括猪圆环病毒2型（PCV2）用于治疗几种临床表现（疾病）的抗原。 优选地，临床表现与PCV2感染相关。 优选地，它们包括淋巴结病，淋巴耗尽和/或多核/巨组织细胞。 更过去，临床症状包括与一种淋巴结肿大或以下症状中的猪的倍数：（1）间质性肺炎并小叶水肿，（2）皮肤苍白或黄疸，（3）斑驳萎缩性肝，（4）胃溃疡 （5）肾炎和（6）生殖病症，例如 流产，死产，木乃伊等。另外更多的临床症状包括皮娅样病变，通常已知的与胞内劳森菌感染有关。

公开(公告)号：EP2281829A3

公开(公告)日：2011-05-04

申请号：EP10174465.4

申请日：2005-12-29

申请人： BOEHRINGER INGELHEIM VETMEDICA, INC.

发明人： Eichmeyer, Marc Allan ,  Schaeffer, Merrill Lynn ,  Nitzel, Greg

IPC分类号： C07K14/01 ,  A61K39/12

CPC分类号： A61K39/12 ,  A61K33/00 ,  A61K45/06 ,  A61K2039/5258 ,  A61K2039/545 ,  A61K2039/552 ,  A61K2039/55505 ,  A61K2039/55555 ,  C07K14/005 ,  C12N2710/14143 ,  C12N2710/14163 ,  C12N2750/10022 ,  C12N2750/10034 ,  C12N2750/10051 ,  C12N2770/10034

摘要： An improved method for recovering the protein expressed by open reading frame 2 from porcine circovirus type 2 is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame 2 coding sequences into cells contained in growth media, causing the virus to express open reading frame 2, and recovering the expressed protein in the supernate. This recovery should take place beginning approximately 5 days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.