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1.发明公开PCV2 immunogenic compositions and methods of producing such compositions 审中-公开PCV2免疫原性组合物和生产这种组合物的方法
公开(公告)号：EP2460820A3
公开(公告)日：2013-01-09
申请号：EP12155382.0
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
摘要翻译： 提供了从猪圆环病毒类型（2）中回收由开放阅读框（2）表达的蛋白质的改进方法。 该方法一般涉及将含有开放阅读框（2）编码序列的重组病毒转染到生长培养基中所含的细胞中，使病毒表达开放阅读框（2），并回收上清中表达的蛋白质。 这种恢复应该在细胞感染大约（5）天后开始，以便允许足量的重组蛋白质从细胞表达并分泌到生长培养基中。 此类方法避免了从细胞内分离和回收重组蛋白所需的昂贵且耗时的提取程序。
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公开(公告)号：EP2460821A3
公开(公告)日：2014-12-10
申请号：EP12156474.4
申请日：2005-12-29
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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3.发明公开PCV2 immunogenic compositions and methods of producing such compositions 审中-公开免疫原性PCV2-Zusammensetzungen和Verfahren zur Herstellung solcher Zusammensetzungen
公开(公告)号：EP2460820A2
公开(公告)日：2012-06-06
申请号：EP12155382.0
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
摘要翻译： 提供了一种从猪圆环病毒型（2）中回收由开放阅读框（2）表达的蛋白质的改进方法。 该方法通常包括将包含开放阅读框（2）的重组病毒转染到生长培养基中包含的细胞中的步骤，使病毒表达开放阅读框（2），并回收上清液中表达的蛋白质。 这种恢复应该在细胞感染后约（5）天开始进行，以便允许足够量的重组蛋白从细胞中表达和分泌到生长培养基中。 这样的方法避免了从细胞内分离和回收重组蛋白质所需的昂贵且耗时的提取方法。
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4.发明授权PRODUCTION OF A HOMOGENEOUS CELL LINE HIGHLY PERMISSIVE TO PORCINE CIRCOVIRUS TYPE 2 (PCV2) INFECTION 有权是生产同质细胞系，高易感染猪肉-2型圆环病毒（PCV2）
公开(公告)号：EP2155246B1
公开(公告)日：2013-03-13
申请号：EP07748678.5
申请日：2007-05-11
IPC分类号： C12N7/00
CPC分类号： C12N7/00 , C12N2750/10052
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5.发明公开PCV2 immunogenic compositions and methods of producing such compositions 审中-公开PCV2免疫原性组合物和生产这种组合物的方法
公开(公告)号：EP2460819A2
公开(公告)日：2012-06-06
申请号：EP12155381.2
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involve the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
摘要翻译： 提供了从猪圆环病毒类型（2）中回收由开放阅读框（2）表达的蛋白质的改进方法。 该方法一般包括将含有开放阅读框（2）编码序列的重组病毒转染到生长培养基中所含的细胞中，使病毒表达开放阅读框（2），并回收表达的上清中的蛋白质。 这种恢复应该在细胞感染大约（5）天后开始，以便允许足量的重组蛋白质从细胞表达并分泌到生长培养基中。 此类方法避免了从细胞内分离和回收重组蛋白所需的昂贵且耗时的提取程序。
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公开(公告)号：EP2460818A2
公开(公告)日：2012-06-06
申请号：EP12155379.6
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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公开(公告)号：EP1379671B1
公开(公告)日：2009-05-06
申请号：EP02712700.0
申请日：2002-03-27
CPC分类号： C07K14/005 , A61K2039/525 , C12N7/00 , C12N15/86 , C12N2710/10322 , C12N2750/10021 , C12N2750/10043 , C12N2750/10052
摘要： The present invention relates to methods for culturing circovirus and in particular, porcine circovirus. The present invention provides compositions and methods for culturing porcine circovirus in mammalian cells expressing mammalian adenovirus E1 function.
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8.发明公开PCV2 immunogenic compositions and methods of producing such compositions 审中-公开PCV2免疫原性组合物和制备这种组合物的方法
公开(公告)号：EP2460819A3
公开(公告)日：2013-01-09
申请号：EP12155381.2
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involve the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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公开(公告)号：EP2460818A3
公开(公告)日：2013-01-09
申请号：EP12155379.6
申请日：2005-12-29
IPC分类号： C07K14/01 , A61K39/12 , C12N7/04 , C12Q1/68 , A61K39/295
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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公开(公告)号：EP2460821A2
公开(公告)日：2012-06-06
申请号：EP12156474.4
申请日：2005-12-29
CPC分类号： A61K33/00 , A61K39/12 , A61K45/06 , A61K2039/5258 , A61K2039/54 , A61K2039/545 , A61K2039/552 , A61K2039/55505 , A61K2039/55555 , A61K2039/55566 , A61K2039/6081 , A61K2039/70 , C07K14/005 , C12N7/00 , C12N2710/14143 , C12N2710/14163 , C12N2750/10022 , C12N2750/10034 , C12N2750/10052 , C12N2770/10034 , C12N2799/026 , C12Q1/701
摘要： An improved method for recovering the protein expressed by open reading frame (2) from porcine circovirus type (2) is provided. The method generally involves the steps of transfecting recombinant virus containing open reading frame (2) coding sequences into cells contained in growth media, causing the virus to express open reading frame (2), and recovering the expressed protein in the supernate. This recovery should take place beginning approximately (5) days after infection of the cells in order to permit sufficient quantities of recombinant protein to be expressed and secreted from the cell into the growth media. Such methods avoid costly and time consuming extraction procedures required to separate and recover the recombinant protein from within the cells.
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