公开(公告)号：EP0664337A1

公开(公告)日：1995-07-26

申请号：EP95104280.3

申请日：1986-09-12

申请人： INSTITUT PASTEUR ,  GENETIC SYSTEMS CORPORATION

发明人： Nowinsky, Robert ,  Montagnier, Luc ,  Klatzmann, David

IPC分类号： C12N7/00 ,  C07K16/10 ,  G01N33/569

CPC分类号： C07K16/1045 ,  C12N1/20 ,  C12N5/0694 ,  C12N7/00 ,  C12N2740/14051 ,  C12N2740/15051 ,  G01N33/56972 ,  G01N33/56977 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974

摘要： Cell lines which lack human class II histocompatibility antigens are disclosed. The cell lines may be utilized within a method for propagating microorganisms, such as viruses, for determining the presence and/or amount of antibody to a microorganism in a biological fluid, and within a method for producing antibodies to a selected microorganism.

摘要翻译： 公开了缺乏人II类组织相容性抗原的细胞系。 细胞系可以在用于繁殖微生物（例如病毒）的方法中用于确定生物流体中对微生物的抗体的存在和/或量的量，以及用于产生针对所选择的微生物的抗体的方法。

公开(公告)号：EP0220970B1

公开(公告)日：1996-01-10

申请号：EP86402005.2

申请日：1986-09-12

申请人： INSTITUT PASTEUR ,  GENETIC SYSTEMS CORPORATION

发明人： Nowinski, Robert ,  Montagnier, Luc ,  Klatzmann, David

IPC分类号： C12N7/00

CPC分类号： C07K16/1045 ,  C12N1/20 ,  C12N5/0694 ,  C12N7/00 ,  C12N2740/14051 ,  C12N2740/15051 ,  G01N33/56972 ,  G01N33/56977 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974

公开(公告)号：EP0220970A3

公开(公告)日：1989-02-08

申请号：EP86402005.2

申请日：1986-09-12

申请人： INSTITUT PASTEUR ,  GENETIC SYSTEMS CORPORATION

发明人： Nowinski, Robert ,  Montagnier, Luc ,  Klatzmann, David

IPC分类号： C12N7/00 ,  C12N1/20 ,  G01N33/569 ,  A61K39/21 ,  C12N5/00

CPC分类号： C07K16/1045 ,  C12N1/20 ,  C12N5/0694 ,  C12N7/00 ,  C12N2740/14051 ,  C12N2740/15051 ,  G01N33/56972 ,  G01N33/56977 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974

摘要： Cell lines which lack human class II histo­compatibility antigens are disclosed. The cell lines may be utilized within a method for propagating micro­organisms, such as viruses, for determining the presence and/or amount of antibody to a microorganism in a biological fluid, and within a method for producing antibodies to a selected microorganism.

公开(公告)号：EP3110831A2

公开(公告)日：2017-01-04

申请号：EP15706804.0

申请日：2015-02-26

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： FAATZ, Elke ,  MUENCH, Peter ,  SCHOLZ, Christian

IPC分类号： C07K14/005 ,  G01N33/569

CPC分类号： G01N33/56988 ,  C07K14/005 ,  C07K2319/35 ,  C12N2740/14022 ,  C12N2740/14031 ,  C12N2740/14051 ,  G01N33/56983 ,  G01N2333/15 ,  G01N2469/20

摘要： The invention concerns soluble and antigenic HTLV p24 variants that are fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising a fragment of at least 25 amino acids of SEQ ID NOs. 1 or 4 (HTLV-1) or SEQ ID NOs. 5 or 8 (HTLV-II), wherein the HTLV p24 antigen is fused to a chaperone. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focusses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.

摘要翻译： 本发明涉及与分子伴侣融合的可溶性和抗原性HTLV p24变体及其在诊断应用中的用途，例如用于检测分离的生物样品中针对HTLV-1或HTLV-II的抗体的免疫测定。 特别地，本发明涉及包含SEQ ID NOs至少25个氨基酸的片段的可溶性HTLV-1或HTLV-II p24抗原。 1或4（HTLV-1）或SEQ ID NO。 5或8（HTLV-II），其中HTLV p24抗原与伴侣融合。 此外，本发明涵盖了编码这些HTLV-1和-II融合抗原的重组DNA分子以及使用表达载体和用这些表达载体转化的宿主细胞的重组生产。 此外，本发明集中于这些HTLV p24抗原与HTLV gp21抗原的组合物和使用本发明的抗原检测HTLV抗体的免疫测定方法。 还包括在体外诊断测定中使用HTLV p24抗原以及用于检测包含所述HTLV抗原的抗HTLV抗体的试剂盒。

公开(公告)号：EP2913338A1

公开(公告)日：2015-09-02

申请号：EP14157165.3

申请日：2014-02-28

申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

发明人： The designation of the inventor has not yet been filed

IPC分类号： C07K14/005 ,  G01N33/569

CPC分类号： G01N33/56988 ,  C07K14/005 ,  C07K2319/35 ,  C12N2740/14022 ,  C12N2740/14031 ,  C12N2740/14051 ,  G01N33/56983 ,  G01N2333/15 ,  G01N2469/20

摘要： The invention concerns soluble and antigenic HTLV p24 variants that are fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising a fragment of at least 25 amino acids of SEQ ID NOs. 1 or 4 (HTLV-1) or SEQ ID NOs. 5 or 8 (HTLV-II), wherein the HTLV p24 antigen is fused to a chaperone. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focusses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.

摘要翻译： 本发明涉及与分子伴侣融合的可溶性和抗原性HTLV p24变体及其在诊断应用中的用途，例如用于检测分离的生物样品中针对HTLV-1或HTLV-II的抗体的免疫测定。 特别地，本发明涉及包含SEQ ID NOs至少25个氨基酸的片段的可溶性HTLV-1或HTLV-II p24抗原。 1或4（HTLV-1）或SEQ ID NO。 5或8（HTLV-II），其中HTLV p24抗原与伴侣融合。 此外，本发明涵盖了编码这些HTLV-1和-II融合抗原的重组DNA分子以及使用表达载体和用这些表达载体转化的宿主细胞的重组生产。 此外，本发明集中于这些HTLV p24抗原与HTLV gp21抗原的组合物和使用本发明的抗原检测HTLV抗体的免疫测定方法。 还包括在体外诊断测定中使用HTLV p24抗原以及用于检测包含所述HTLV抗原的抗HTLV抗体的试剂盒。

公开(公告)号：EP0220970A2

公开(公告)日：1987-05-06

申请号：EP86402005.2

申请日：1986-09-12

申请人： INSTITUT PASTEUR ,  GENETIC SYSTEMS CORPORATION

发明人： Nowinski, Robert ,  Montagnier, Luc ,  Klatzmann, David

IPC分类号： C12N7/00 ,  C12N1/20 ,  G01N33/569 ,  A61K39/21 ,  C12N5/00

CPC分类号： C07K16/1045 ,  C12N1/20 ,  C12N5/0694 ,  C12N7/00 ,  C12N2740/14051 ,  C12N2740/15051 ,  G01N33/56972 ,  G01N33/56977 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974

摘要： Cell lines which lack human class II histo­compatibility antigens are disclosed. The cell lines may be utilized within a method for propagating micro­organisms, such as viruses, for determining the presence and/or amount of antibody to a microorganism in a biological fluid, and within a method for producing antibodies to a selected microorganism.

摘要翻译： 公开了缺乏人II类组织相容性抗原的细胞系。 细胞系可以在用于繁殖微生物（例如病毒）的方法中用于确定生物流体中对微生物的抗体的存在和/或量的量，以及用于产生针对所选择的微生物的抗体的方法。

公开(公告)号：EP3110831B1

公开(公告)日：2018-01-10

申请号：EP15706804

申请日：2015-02-26

申请人： ROCHE DIAGNOSTICS GMBH ,  F HOFFMANN-LA ROCHE AG

发明人： FAATZ ELKE ,  MUENCH PETER ,  SCHOLZ CHRISTIAN

IPC分类号： C07K14/005 ,  G01N33/569

CPC分类号： G01N33/56988 ,  C07K14/005 ,  C07K2319/35 ,  C12N2740/14022 ,  C12N2740/14031 ,  C12N2740/14051 ,  G01N33/56983 ,  G01N2333/15 ,  G01N2469/20

摘要： The invention concerns soluble and antigenic HTLV p24 variants that are fused to chaperones and their use in diagnostic applications such as immunoassays for detecting antibodies against HTLV-I or HTLV-II in an isolated biological sample. In particular, the invention relates to a soluble HTLV-I or HTLV-II p24 antigen comprising a fragment of at least 25 amino acids of SEQ ID NOs. 1 or 4 (HTLV-1) or SEQ ID NOs. 5 or 8 (HTLV-II), wherein the HTLV p24 antigen is fused to a chaperone. Moreover, the invention covers recombinant DNA molecules encoding these HTLV-I and -II fusion antigens as well as their recombinant production using expression vectors and host cells transformed with such expression vectors. In addition, the invention focusses on compositions of these HTLV p24 antigens with HTLV gp21 antigen and on an immunoassay method for detection of HTLV antibodies using the antigens of the invention. Also the use of HTLV p24 antigens in an in vitro diagnostic assay as well as a reagent kit for detection of anti-HTLV-antibodies comprising said HTLV antigens is encompassed.

公开(公告)号：EP0790836A4

公开(公告)日：2003-05-28

申请号：EP95941434

申请日：1995-11-30

申请人： DYNCORP ,  NAT INST HEALTH

发明人： CHANOCK ROBERT M ,  PURCELL ROBERT H ,  KAPIKIAN ALBERT Z ,  POTASH LOUIS

IPC分类号： C12N5/00 ,  A61K39/12 ,  A61K39/125 ,  A61K39/13 ,  A61K39/15 ,  A61K39/29 ,  A61P31/12 ,  C12N7/00 ,  C12N7/04 ,  A61K39/145 ,  C12N5/06 ,  C12N7/02

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  C12N2720/12334 ,  C12N2720/12351 ,  C12N2740/10051 ,  C12N2740/14051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/18534 ,  C12N2760/18551 ,  C12N2760/18634 ,  C12N2760/18651 ,  C12N2760/18734 ,  C12N2760/18751 ,  C12N2770/12034 ,  C12N2770/12051 ,  C12N2770/32334 ,  C12N2770/32351 ,  C12N2770/32434 ,  C12N2770/32451 ,  C12N2770/32634 ,  C12N2770/32651

摘要： A novel African Green Monkey Kidney (AGMK) cell line is taught as well as a method for the preparation thereof. The cell line which is free of viable adventitious microbial agents is useful as a substrate for viruses and for the preparation of viral vaccines.

公开(公告)号：EP0790836A1

公开(公告)日：1997-08-27

申请号：EP95941434.0

申请日：1995-11-30

申请人： DYNCORP ,  NATIONAL INSTITUTES OF HEALTH

发明人： CHANOCK, Robert M. ,  PURCELL, Robert H. ,  KAPIKIAN, Albert Z. ,  POTASH, Louis

IPC分类号： C12N5 ,  A61K39 ,  A61P31 ,  C12N7

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5254 ,  C12N2720/12334 ,  C12N2720/12351 ,  C12N2740/10051 ,  C12N2740/14051 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/18534 ,  C12N2760/18551 ,  C12N2760/18634 ,  C12N2760/18651 ,  C12N2760/18734 ,  C12N2760/18751 ,  C12N2770/12034 ,  C12N2770/12051 ,  C12N2770/32334 ,  C12N2770/32351 ,  C12N2770/32434 ,  C12N2770/32451 ,  C12N2770/32634 ,  C12N2770/32651 ,  Y02A50/466

摘要： A novel African Green Monkey Kidney (AGMK) cell line is taught as well as a method for the preparation thereof. The cell line which is free of viable adventitious microbial agents is useful as a substrate for viruses and for the preparation of viral vaccines.

公开(公告)号：EP0646793A3

公开(公告)日：1995-07-12

申请号：EP94115622.6

申请日：1986-09-12

申请人： INSTITUT PASTEUR ,  GENETIC SYSTEMS CORPORATION

发明人： Nowinski, Robert ,  Montagnier, Luc ,  Klatzmann, David

IPC分类号： G01N33/569

CPC分类号： C07K16/1045 ,  C12N1/20 ,  C12N5/0694 ,  C12N7/00 ,  C12N2740/14051 ,  C12N2740/15051 ,  G01N33/56972 ,  G01N33/56977 ,  G01N33/56983 ,  G01N33/56988 ,  Y10S435/974

摘要： Cell lines which lack human class II histocompatibility antigens are disclosed. The cell lines may be utilized within a method for propagating microorganisms, such as viruses, for determining the presence and/or amount of antibody to a microorganism in a biological fluid, and within a method for producing antibodies to a selected microorganism.

摘要翻译： 公开了缺乏人II类组织相容性抗原的细胞系。 细胞系可以在用于繁殖微生物（例如病毒）的方法中用于确定生物流体中对微生物的抗体的存在和/或量的量，以及用于产生针对所选择的微生物的抗体的方法。