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    1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE
    4.
    发明公开
    1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE 审中-公开
    FUR HERSTELLUNGSVERFAHREN 1,4-丁二醇，Mikroorganismus UND JAN

    公开(公告)号：EP2930239A4

    公开(公告)日：2016-06-29

    申请号：EP13859911

    申请日：2013-11-28

    申请人： SHOWA DENKO KK

    发明人： AOKI HIROBUMI ,  KOKIDO YUZURU ,  HASHIMOTO YOKO ,  YONEDA TADASHI

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P7/18

    CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y203/01009 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y402/01119 ,  C12Y402/0112 ,  C12Y503/03003

    摘要： A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.

    摘要翻译： 通过使用微生物和/或其培养物，通过乙酰辅酶A，乙酰乙酰辅酶A，3-羟基丁酰基-CoA，巴豆酰基-CoA，和4-羟基丁酰-CoA的制造1,4-丁二醇的方法，其中，在所述微生物 对于1,4-丁二醇的制造方法包括：间基因之一年（a）该基因具有序列号1的碱基序列，（b）将所述基因具有的碱基序列，例如阙一个或多个碱基缺失，取代， 或在序列号1的碱基序列，其中所述基因具有大于或等于90％，相对于序列号1的碱基序列的同一性的碱基序列，和（c）的基因阙与基因杂交加入 具有与阙对严格条件在序列号1中记载的碱基序列的基因互补的碱基序列，并且包括其中（d）中的基因的任何一种或多种基因阙具有2至9的序列号的碱基序列，（e）中 该基因具有的碱基序列，例如阙一个或多个碱基缺失，取代，或添加在 序列编号2〜9，其中，所述基因具有的碱基序列具有标识大于或等于90％，相对于它的原始碱基序列，和（f）的基因阙与基因杂交阙具有基因阙互补的碱基序列的碱基序列 有序列号在严格条件2〜9的碱基序列。

    METHOD FOR PRODUCING 1,4-BUTANEDIOL, AND MICROORGANISM
    7.
    发明公开
    METHOD FOR PRODUCING 1,4-BUTANEDIOL, AND MICROORGANISM 审中-公开
    密克罗尼西亚VERFAHREN ZUR HERSTELLUNG VON 1,4-BUTANDIOL

    公开(公告)号：EP2927325A1

    公开(公告)日：2015-10-07

    申请号：EP13857866.1

    申请日：2013-09-20

    申请人： Showa Denko K.K.

    发明人： AOKI, Hirobumi ,  KOKIDO, Yuzuru ,  HASHIMOTO, Yoko ,  YONEDA, Tadashi

    IPC分类号： C12P7/18 ,  C12N1/19 ,  C12N1/21 ,  C12N15/09

    CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y402/0112 ,  C12Y503/03003

    摘要： A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.

    摘要翻译： 通过3-羟基丁酰辅酶A，巴豆酰辅酶A和4-羟基丁酰辅酶A的酶反应体系依次使用微生物和/或其培养物制备1,4-丁二醇的方法，其中3-羟基丁酰基 - CoA是光学活性物质，其中微生物包括（1）编码烯酰辅酶A水合酶的基因，（2）编码乙酰乙酰辅酶Aδ异构酶的基因，（3）编码4-羟基丁酰辅酶A脱水酶 ，（4）编码酰基辅酶A还原酶的基因，其底物特异性具有与3-羟基丁酰辅酶A相反的光学选择性。

    1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE
    9.
    发明公开
    1,4-BUTANEDIOL PRODUCTION METHOD, MICROORGANISM, AND GENE 审中-公开
    法生产1,4-丁二醇，微生物及GEN

    公开(公告)号：EP2930239A1

    公开(公告)日：2015-10-14

    申请号：EP13859911.3

    申请日：2013-11-28

    申请人： Showa Denko K.K.

    发明人： AOKI, Hirobumi ,  KOKIDO, Yuzuru ,  HASHIMOTO, Yoko ,  YONEDA, Tadashi

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12P7/18

    CPC分类号： C12P7/18 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12Y101/01035 ,  C12Y101/01036 ,  C12Y101/01157 ,  C12Y203/01009 ,  C12Y402/01017 ,  C12Y402/01055 ,  C12Y402/01119 ,  C12Y402/0112 ,  C12Y503/03003

    摘要： A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequence numbers 2 to 9, wherein the genes have base sequences with an identity greater than or equal to 90% with respect to original base sequences thereof, and (f) genes that hybridize with genes that have base sequences complementary with genes that have base sequences of sequence numbers 2 to 9 on a stringent condition.