摘要:
An axial light loss sensor system, and methods for measuring axial light loss with improved resolution are provided. Aspects of the present invention include an axial light loss sensor positioned along an axis of irradiation to detect axial light loss resulting from a particle passing a light source intersect in a fluid stream, and an obstruction positioned along the axis of irradiation between the light source intersect and the axial light loss sensor. The obstruction is further positioned so as to have an on-axis opaque surface. The obstruction allows for the measurement of a fringe signal in a far-field with respect to the irradiated particle, in order to measure the axial light loss produced by the particle. The systems and methods described herein find use in, for example, flow cytometery.
摘要:
This blood examination apparatus examines cancer cells mixed in an examination object which is flowing blood, and includes: a flow cell through which the examination object is made to flow; an imaging optical system which light output from the examination object in an examination region in the flow cell enters, the imaging optical system forming an image of the light on a first image plane; a first Fourier transformation optical system which optically two-dimensionally Fourier-transforms the image formed on the first image plane by the imaging optical system to form the Fourier-transformed image on a second image plane; a spatial light filter which selectively allows a portion in a certain range around an optical axis of the first Fourier transformation optical system of the image formed on the second image plane by the first Fourier transformation optical system to pass through; and a second Fourier transformation optical system which optically two-dimensionally Fourier-transforms the portion which has passed through the spatial light filter of the image formed on the second image plane by the first Fourier transformation optical system to form the Fourier-transformed image on a third image plane.
摘要:
The invention relates to a method for measuring the speed of a particle such as a red blood cell moving inside a flow such as a flow of the blood, using a light scanning microscope. The inventive method comprises the following steps: acquisition of an image by x and y light scanning on a plane containing the object; detection on the plane (x, y) of a mark on the plane (x, y); estimation of the speed vg of the object from the gradient thus determined.
摘要:
Dans un appareil à laser-doppler la taille de particules sphériques mobiles dans un fluide en écoulement est déterminée par le croisement de deux rayons laser séparés dans l'espace (15, 16) et se coupant suivant un angle donné () dans un volume de mesure traversé par une partie du fluide en écoulement. A l'aide de deux détecteurs photoélectriques de mesure (17, 18) disposés dans des positions différentes dans l'espace à l'extérieur du volume de mesure, les rayons diffusés par une particule mobile sont détectés sous la forme de signaux doppler possédant une phase différente. La taille des particules est déterminée en produisant un signal de différence de phase résultant (PHIM) à partir des signaux produits par les deux détecteurs de mesure (17, 18). En combinant le signal provenant d'un détecteur de mesure (17) avec le signal provenant d'un détecteur de référence (19) positionné entre les deux détecteurs de mesure, on produit un signal de référence de phase (PHIR) indiquant si le signal de différence de phase résultant (PHIM) a été produit par une différence de phase entre les signaux doppler dépassant 2pi.
摘要:
A method of detecting particles (1), e. g. proteins, after separation of particles based on their specific features, e.g. charge, size, shape, density, as series of single light scattering events created by the individual particles is described. The particles (1) are separated from each other along the separation path (11) and particles have specific arrival times at the target side depending on the particle features. The detecting step comprises an interferometric sensing of the light scattered at individual particles bound or transient in the detection volume (30). Parameters of the scattering light signals e.g. the interferometric contrast are analysed for obtaining specific particle features, e.g. size, mass, shape, charge, or affinity of the particles (1). Furthermore, a detection apparatus (100) being configured for detecting particles (1) is described.
摘要:
A flow cytometry system having a flow channel defined through the thickness of a substrate is disclosed. Fluid flowing through the flow channel is illuminated by a first plurality of surface waveguides that are arranged around the flow channel in a first plane, while a second plurality of surface waveguides arranged around the flow channel in a second plane receive light after it has interacted with the fluid. The illumination pattern provided to the fluid is controlled by controlling the phase of the light in the first plurality of surface waveguides. As a result, the fluid is illuminated with light that is uniform and has a low coefficient of variation, improving the ability to distinguish and quantify characteristics of the fluid, such as cell count, DNA content, and the like.