AXIAL LIGHT LOSS SENSOR SYSTEM FOR FLOW CYTOMETERY
    3.
    发明公开
    AXIAL LIGHT LOSS SENSOR SYSTEM FOR FLOW CYTOMETERY 审中-公开
    AXIALLICHTVERLUST传感器系统流式细胞仪

    公开(公告)号:EP2702389A1

    公开(公告)日:2014-03-05

    申请号:EP12776687.1

    申请日:2012-04-25

    IPC分类号: G01N15/14 G01N21/47

    摘要: An axial light loss sensor system, and methods for measuring axial light loss with improved resolution are provided. Aspects of the present invention include an axial light loss sensor positioned along an axis of irradiation to detect axial light loss resulting from a particle passing a light source intersect in a fluid stream, and an obstruction positioned along the axis of irradiation between the light source intersect and the axial light loss sensor. The obstruction is further positioned so as to have an on-axis opaque surface. The obstruction allows for the measurement of a fringe signal in a far-field with respect to the irradiated particle, in order to measure the axial light loss produced by the particle. The systems and methods described herein find use in, for example, flow cytometery.

    BLOOD EXAMINATION APPARATUS
    5.
    发明公开
    BLOOD EXAMINATION APPARATUS 有权
    BLUTUNTERSUCHUNGSVORRICHTUNG

    公开(公告)号:EP2187198A1

    公开(公告)日:2010-05-19

    申请号:EP08778292.6

    申请日:2008-07-18

    IPC分类号: G01N21/17 G01N33/49

    摘要: This blood examination apparatus examines cancer cells mixed in an examination object which is flowing blood, and includes: a flow cell through which the examination object is made to flow; an imaging optical system which light output from the examination object in an examination region in the flow cell enters, the imaging optical system forming an image of the light on a first image plane; a first Fourier transformation optical system which optically two-dimensionally Fourier-transforms the image formed on the first image plane by the imaging optical system to form the Fourier-transformed image on a second image plane; a spatial light filter which selectively allows a portion in a certain range around an optical axis of the first Fourier transformation optical system of the image formed on the second image plane by the first Fourier transformation optical system to pass through; and a second Fourier transformation optical system which optically two-dimensionally Fourier-transforms the portion which has passed through the spatial light filter of the image formed on the second image plane by the first Fourier transformation optical system to form the Fourier-transformed image on a third image plane.

    摘要翻译: 该血液检查装置检查在流动的血液的检查对象物中混合的癌细胞,并且包括:使检查对象流过的流通池; 在流动池中的检查区域中从检查对象输出的成像光学系统进入成像光学系统,在第一图像平面上形成光的图像; 第一傅立叶变换光学系统,其通过所述成像光学系统光学地二维地傅里叶变换在所述第一图像平面上形成的图像,以在第二图像平面上形成所述傅里叶变换图像; 空间光滤波器,其通过第一傅里叶变换光学系统选择性地允许在第二图像平面上形成的图像的第一傅立叶变换光学系统的光轴周围的一定范围内的部分通过; 以及第二傅里叶变换光学系统,其通过第一傅立叶变换光学系统对通过第二图像平面上形成的图像的空间滤光器的部分进行光学二维傅立叶变换,以形成傅立叶变换图像 第三象机。

    A LASER-DOPPLER-APPARATUS FOR DETERMINING THE SIZE OF MOVING SPHERICAL PARTICLES IN A FLUID FLOW
    7.
    发明公开
    A LASER-DOPPLER-APPARATUS FOR DETERMINING THE SIZE OF MOVING SPHERICAL PARTICLES IN A FLUID FLOW 失效
    激光多普勒装置用于确定SIZE运动的粒子在液体付款。

    公开(公告)号:EP0144310A1

    公开(公告)日:1985-06-19

    申请号:EP83901590.0

    申请日:1983-05-18

    IPC分类号: G01N15

    CPC分类号: G01N15/0205 G01N2015/145

    摘要: Dans un appareil à laser-doppler la taille de particules sphériques mobiles dans un fluide en écoulement est déterminée par le croisement de deux rayons laser séparés dans l'espace (15, 16) et se coupant suivant un angle donné () dans un volume de mesure traversé par une partie du fluide en écoulement. A l'aide de deux détecteurs photoélectriques de mesure (17, 18) disposés dans des positions différentes dans l'espace à l'extérieur du volume de mesure, les rayons diffusés par une particule mobile sont détectés sous la forme de signaux doppler possédant une phase différente. La taille des particules est déterminée en produisant un signal de différence de phase résultant (PHIM) à partir des signaux produits par les deux détecteurs de mesure (17, 18). En combinant le signal provenant d'un détecteur de mesure (17) avec le signal provenant d'un détecteur de référence (19) positionné entre les deux détecteurs de mesure, on produit un signal de référence de phase (PHIR) indiquant si le signal de différence de phase résultant (PHIM) a été produit par une différence de phase entre les signaux doppler dépassant 2pi.

    FLOW CYTOMETRY SYSTEM AND METHOD
    9.
    发明公开
    FLOW CYTOMETRY SYSTEM AND METHOD 审中-公开
    流式细胞术系统和方法

    公开(公告)号:EP3164692A1

    公开(公告)日:2017-05-10

    申请号:EP15734644.6

    申请日:2015-07-01

    申请人: Octrolix BV

    IPC分类号: G01N15/14 G01N21/05 G01N21/03

    摘要: A flow cytometry system having a flow channel defined through the thickness of a substrate is disclosed. Fluid flowing through the flow channel is illuminated by a first plurality of surface waveguides that are arranged around the flow channel in a first plane, while a second plurality of surface waveguides arranged around the flow channel in a second plane receive light after it has interacted with the fluid. The illumination pattern provided to the fluid is controlled by controlling the phase of the light in the first plurality of surface waveguides. As a result, the fluid is illuminated with light that is uniform and has a low coefficient of variation, improving the ability to distinguish and quantify characteristics of the fluid, such as cell count, DNA content, and the like.

    摘要翻译: 公开了具有通过衬底厚度限定的流动通道的流式细胞术系统。 流过流动通道的流体由第一多个表面波导来照明,该第一多个表面波导在第一平面中围绕流动通道布置,而围绕第二平面布置在流动通道周围的第二多个表面波导在与第一平面相互作用之后接收光 流体。 提供给流体的照射图案通过控制第一多个表面波导中的光的相位来控制。 结果,流体被均匀且具有低变异系数的光照射,提高了区分和量化流体特性(例如细胞计数,DNA含量等)的能力。