公开(公告)号：EP2654072A3

公开(公告)日：2016-01-27

申请号：EP13000797.4

申请日：2013-02-15

申请人： Bruker Daltonics, Inc.

发明人： Park, Melvin ,  Kaplan, Desmond

IPC分类号： H01J49/00

CPC分类号： H01J49/0054

摘要： The invention relates to a device for performing electron capture dissociation on multiply charged cations. Provided is an electron emitter which, upon triggering, emits a plurality of low energy electrons suitable for efficient electron capture reactions to occur. Further, the device contains a particle emitter being located proximate to the electron emitter and being capable, upon triggering, to emit a plurality of high energy charged particles substantially in a direction towards the electron emitter in order that the electron emitter receives a portion of the emitted plurality of high energy charged particles and emission of the plurality of low energy electrons is triggered. A volume capable of containing a plurality of multiply charged cations is located in opposing relation to the electron emitter such that the volume receives the plurality of low energy electrons upon emission as to allow electron capture dissociation to occur.

公开(公告)号：EP1371083B1

公开(公告)日：2006-03-22

申请号：EP02718000.9

申请日：2002-03-22

申请人： Syddansk Universitet

发明人： ZUBAREV, Roman

IPC分类号： H01J49/42 ,  H01J49/10

CPC分类号： H01J49/0054

摘要： Methods and apparatus are provided to obtain efficient Electron capture dissociation (ECD) of positive ions, particularly useful in the mass spectrometric analysis of complex samples such as of complex mixtures and large biomolecules of peptides and proteins. Due to the low efficiency of ECD as previously used, the technique has so far only been employed with Penning cell ion cyclotron resonance mass spectrometers, where the ions are confined by a combination of magnetic and electrostatic fields. To substantially increase the efficiency of electron capture, the invention makes use of a high-intensity electron source producing a high-flux low-energy electron beam of a diameter comparable to that of the confinement volume of ions. Such a beam possesses trapping properties for positive ions. The ions confined by electron beam effectively capture electrons, which leads much shorter analysis time. The invention provides the possibility to employs ECD in other trapping and non-trapping instruments beside ICR mass spectrometers.

公开(公告)号：EP1598850A2

公开(公告)日：2005-11-23

申请号：EP05010587.3

申请日：2005-05-17

申请人： Bruker Daltonik GmbH

发明人： Franzen, Jochen Dr.

IPC分类号： H01J49/42

CPC分类号： H01J49/421 ,  H01J49/0054

摘要： The invention relates to a mass spectrometer, in particular a time-of-flight mass spectrometer, with fragmentation of pre-selected molecule ions for the acquisition of daughter ion spectra.
The invention consists in an instrument with devices for generating multiply charged ions of the molecule of interest, for selecting a multiply charged ion species by a mass filter, for fragmenting the selected ions by allowing them to react with low energy electrons in an RF ion guide system, and for analyzing the fragment ions in a mass analyzer.

摘要翻译： 本发明涉及一种质谱仪，特别是飞行时间质谱仪，其具有用于获取子离子光谱的预选分子离子的碎裂。 本发明包括具有用于产生感兴趣的分子的多电荷离子的装置的仪器，用于通过质量过滤器选择多重带电的离子种类，以通过允许它们与RF离子导向器中的低能电子反应来使所选择的离子碎裂 系统，并用于分析质量分析仪中的碎片离子。

公开(公告)号：EP1549923A2

公开(公告)日：2005-07-06

申请号：EP03756376.4

申请日：2003-05-30

申请人： ANALYTICA OF BRANFORD, INC.

发明人： WHITEHOUSE, Craig, M.Analytica of Branford, Inc. ,  WELKIE, David, G.c/o Analytica of Branford, Inc. ,  GHOLAMREZA, JavaheryAnalytica of Branford, Inc. ,  COUSINS, Lisac/o Analytica of Branford, Inc. ,  RAKOV, Sergeyc/o Analytica of Branford, Inc.

IPC分类号： G01N1/00

CPC分类号： H01J49/063 ,  H01J49/0054

摘要： A method and apparatus for ion fragmentation comprising an RF multipole collision cell (3) where precursor ions are extracted from and transported through RF multipole ion guide (24), said ions being formed by electron capture reaction with low energy electrons generated in low energy electron source (5).

公开(公告)号：EP3241231A1

公开(公告)日：2017-11-08

申请号：EP15875330.1

申请日：2015-12-21

申请人： DH Technologies Development Pte. Ltd.

发明人： BABA, Takashi

IPC分类号： H01J49/40

CPC分类号： H01J49/0072 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/063

摘要： A method and apparatus for conducting reactions between precursor ions and reagent ions, for example, a reaction between a precursor cation and an electron, such as ECD, are disclosed. The apparatus comprises first, second, and third pathways, each of which extends at least partially along a central axis, and wherein the second central axis is orthogonal to the first and third central axes. Charged species can be introduced into the second pathway as the ions are transmitted therethrough, thereby increasing precursor ion and charged species interaction without simultaneous trapping of the species.

公开(公告)号：EP3055876A1

公开(公告)日：2016-08-17

申请号：EP14784360.1

申请日：2014-10-09

申请人： Micromass UK Limited

发明人： BROWN, Jeffery Mark

IPC分类号： H01J49/00

CPC分类号： H01J49/0072 ,  H01J49/0054 ,  H01J49/164

摘要： A method of mass spectrometry is disclosed comprising: providing supercharged analyte ions; and supplying electrons or reagent ions to said analyte ions so as to transfer charge from said reagent ions or electrons to said analyte ions, said transfer of charge causing at least some of said analyte ions to dissociate. The charge transfer step is performed at a relatively high pressure and preferably substantially at atmospheric pressure.

公开(公告)号：EP3014278A1

公开(公告)日：2016-05-04

申请号：EP14750536.6

申请日：2014-06-24

申请人： Institut National de la Recherche Agronomique (INRA)

发明人： GIULIANI, Alexandre

IPC分类号： G01N33/68 ,  H01J49/00

CPC分类号： H01J49/0077 ,  G01N30/7266 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0045 ,  H01J49/0054 ,  H01J49/0072 ,  H01J49/4225

摘要： The present invention concerns a system and method of mass spectrometry, comprising the following steps: a. ionising an analyte to form a precursor ion (A) having a mass-to-charge ratio (m/z), in which m represents the mass and z the atomic number; b. activating the precursor ion (A) by interaction with a beam of neutral species, ions, electrons or photons, having an energy chosen on the basis of the physicochemical properties of the precursor ion, said activation being suitable for producing a product ion (B, C) having the same mass m as said precursor ion (A) and an atomic number z' such that z' is a non-zero integer different from z, z' preferably being chosen from z+1,
z+2, z+3, z-1, z-2, z-3, the product ion being formed by ionisation, electron detachment, electron capture or transfer without fragmentation; c. separating the product ion (B, C, E, F) having a predefined mass-to-charge ratio (m/z'); d. detecting said product ion (B, C) having the predefined mass-to-charge ratio (m/z').

公开(公告)号：EP2883237A1

公开(公告)日：2015-06-17

申请号：EP13829969.8

申请日：2013-08-15

申请人： The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of Oregon State University

发明人： BAROFSKY, Douglas, F. ,  VOINOV, Valery, G.

IPC分类号： H01J49/08 ,  H01J49/26

CPC分类号： H01J49/147 ,  H01J49/0054 ,  H01J49/062 ,  H01J49/08 ,  H01J49/26

摘要： An electron source for electron-induced dissociation in an RF-free electromagnetostatic cell for use installation in a tandem mass spectrometer is provided. An electromagnetostatic electron-induced dissociation cell may include at least one magnet having an opening disposed therein and having a longitudinal axis extending through the opening, the magnet having magnetic flux lines associated therewith, and an electron emitter having an electron emissive surface comprising a sheet, the emitter disposed about the axis at a location relative to the magnet where the electron emissive surface is substantially perpendicular to the magnetic flux lines at the electron emissive surface.

公开(公告)号：EP2834836A2

公开(公告)日：2015-02-11

申请号：EP13715436.5

申请日：2013-04-05

申请人： Micromass UK Limited ,  The University of British Columbia

发明人： BROWN, Jeffery Mark ,  ROBB, Damon

IPC分类号： H01J49/00

CPC分类号： H01J49/0054 ,  H01J49/0031 ,  H01J49/0072

摘要： A method of mass spectrometry is disclosed comprising providing a mixture of different analyte ions and supplying electrons or reagent ions to said mixture so as to transfer charge to the analyte ions. The transfer of charge causes at least some of the analyte ions to dissociate and others of the analyte ions not to dissociate, but to form intermediate ions of altered charge state. These intermediate ions are then isolated from other ions and excited so as to dissociate into daughter ions. The intermediate ions and their daughter ions are analyzed and associated with each other so that the intermediate can be identified from their daughter ions. The analyte ions can then be identified from the intermediate ions, since they differ only in charge state. The disclosed method enables analyte ions to be associated with their fragment ions, and therefore identified, without having to isolate individual analyte ions prior to their interactions with the electrons or reagent ions.

公开(公告)号：EP2654072A2

公开(公告)日：2013-10-23

申请号：EP13000797.4

申请日：2013-02-15

申请人： Bruker Daltonics, Inc.

发明人： Park, Melvin ,  Kaplan, Desmond

IPC分类号： H01J49/00

CPC分类号： H01J49/0054

摘要： The invention relates to a device for performing electron capture dissociation on multiply charged cations. Provided is an electron emitter which, upon triggering, emits a plurality of low energy electrons suitable for efficient electron capture reactions to occur. Further, the device contains a particle emitter being located proximate to the electron emitter and being capable, upon triggering, to emit a plurality of high energy charged particles substantially in a direction towards the electron emitter in order that the electron emitter receives a portion of the emitted plurality of high energy charged particles and emission of the plurality of low energy electrons is triggered. A volume capable of containing a plurality of multiply charged cations is located in opposing relation to the electron emitter such that the volume receives the plurality of low energy electrons upon emission as to allow electron capture dissociation to occur.

摘要翻译： 本发明涉及用于在多电荷阳离子上进行电子俘获解离的装置。 提供了一种电子发射器，其在触发时发射适合于发生有效电子捕获反应的多个低能电子。 此外，该器件包含位于电子发射器附近的粒子发射器，并且能够在触发时基本上沿朝向电子发射器的方向发射多个高能量带电粒子，以使电子发射器接收一部分 发射多个高能量带电粒子并发射多个低能电子。 能够容纳多个多电荷阳离子的体积位于与电子发射体相对的关系中，使得体积在发射时接收多个低能电子以允许发生电子俘获解离。