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    METHOD FOR CONSTRUCTING HEPATIC PROGENITOR CELL-LIKE CELL BANK, CELL LINES PREPARED THEREFROM AND APPLICATION THEREOF
    5.
    发明公开
    METHOD FOR CONSTRUCTING HEPATIC PROGENITOR CELL-LIKE CELL BANK, CELL LINES PREPARED THEREFROM AND APPLICATION THEREOF 审中-公开

    公开(公告)号：EP3978600A1

    公开(公告)日：2022-04-06

    申请号：EP20830058.2

    申请日：2020-04-24

    申请人： Shanghai Celliver Biotechnology Co., Ltd. ,  Shanghai Cryowise Medical Technology Co., Ltd.

    发明人： YAN, Hexin

    IPC分类号： C12N5/22 ,  C12N5/071 ,  A61K35/12 ,  A61K35/407

    摘要： The invention provides a method for constructing a hepatic progenitor cell-like cell bank, including successively performing following processes to human primary hepatocyte cultures from different donor sources: transformation-culture, cryopreservation treatment, proliferation-culture, a first subculture treatment, virus infection, a second subculture treatment, continuous selection-culture and continuous subculture. In the method for constructing a heterogenous immortal hepatic progenitor cell-like cell bank of the present invention, the human primary hepatocyte culture of each of the donor sources is transformation-cultured before the proliferation-culture, which is beneficial in endowing the human primary hepatocyte cultures with good proliferation performance. Once combined with subsequent controlling of culture parameter, the immortal hepatic progenitor cell-like cell lines obtained from different donor sources may have good in vitro proliferation ability. The invention also provids an application of the hepatic progenitor cell-like cell bank and cell lines obtained by the construction method.