摘要:
PROBLEM TO BE SOLVED: To provide polynucleotides encoding antigenic HIV polypeptides, formulations of immunogenic compositions thereof and uses thereof as well as methods of using these polynucleotides and polypeptide products therefrom for the treatment of acquired immune deficiency syndrome (AIDS). SOLUTION: Provided are polynucleotides encoding immunogenic HIV polypeptides; uses of the polynucleotides in applications including immunization, generation of packaging cell lines, and production of HIV polypeptides; polynucleotides encoding antigenic HIV polypeptides; uses of these polynucleotides and polypeptide products derived therefrom; and isolated wild-type polynucleotides and/or expression cassettes encoding HIV polypeptides (Env, Gag, Pol, Prot, Rt, Int, Vpr, Vpu, Vif, Nef, Tat, Rev and/or combinations and fragments thereof) in a specific embodiment. COPYRIGHT: (C)2009,JPO&INPIT
摘要:
The present invention relates to vectors and their use in gene transfer, In particular the invention provides a vector comprising nucleotides corresponding to the packaging nucleotides of a lentivirus, a heterologous gene, and, flanking the packaging nucleotide and the heterologous gene, sequences corresponding to those within and near the sufficient lentivirus LTR, for packaging, reverse transcription and integration of the vector into target cells and expression of the heterologous gene wherein said nucleotides comprise those corresponding at least substantially to the lentivirus 5' leader sequence and REV-responsive region.
摘要:
PROBLEM TO BE SOLVED: To provide a novel composition and method for treating a variety of diseases (for example, viral diseases, cancer, and hereditary diseases, etc) and furthermore other related benefit. SOLUTION: Disclosed is a retroviral vector infecting to human cells, wherein the retroviral vector contains a first recombinant gene and a second recombinant gene operatively linked to a control sequence. The first recombinant gene is a gene encoding a product which activates a compound without almost or complete cytotoxicity (prodrug) into a toxic agent. The second recombinant gene is a vector encoding a human cytokine. COPYRIGHT: (C)2005,JPO&NCIPI
摘要:
The present invention provides novel lentiviral packaging constructs that are useful for the establishment of stable packaging cell lines and producer cell lines. In particular, the present invention provides novel packaging cell lines that are capable of constitutively expressing high levels of lentiviral proteins.
摘要:
PROBLEM TO BE SOLVED: To provide a method allowing production of recombinant retroviruses (retroviral vector particles) from producer cells which are safer and of higher titer than normal. SOLUTION: A method for selecting a packaging cell producing a high level main factor selected from the group consisting of packaging proteins and target gene products comprises (a) supplying a genome comprising a main gene expressing the main factor in a cell and a selectable gene expressing a selectable protein in a cell to the packaging cell, herein the selectable gene is arranged in the downstream of the main gene and as a result is expressed at a lower level than that of the main gene, (b) exposing the packaging cell to a selection factor enabling identification of a cell expressing the selectable protein at a critical level, and (c) detecting a packaging cell expressing a higher level main factor. COPYRIGHT: (C)2004,JPO
摘要:
PROBLEM TO BE SOLVED: To provide a vector applicable to treatment using gene transmission. SOLUTION: The vector comprises one comprising a nucleotide corresponding to a packaging nucleotide of lentivirus, a heterogenous gene and a sequence, adjacent to the nucleotide and the heterogenous gene, corresponding to internal or intimate lentivirus LTR sufficient to reverse transfer to the packaging and the target cells of the vector and to express heterogeneous gene, wherein the nucleotide comprises one practically corresponding to at least lentivirus 5'-leader sequence and REV-active zone. COPYRIGHT: (C)2004,JPO