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210 条记录 (耗时 0.047 秒)

    Method of identifying compound interacting with transmembrane protein
    85.
    发明专利
    Method of identifying compound interacting with transmembrane protein 有权
    鉴定化合物与转化蛋白相互作用的方法

    公开(公告)号：JP2010112955A

    公开(公告)日：2010-05-20

    申请号：JP2009267007

    申请日：2009-11-25

    申请人： Patobios Ltd ,  パトバイオス リミテッド

    发明人： O'DOWD BRIAN F ,  GEORGE SUSAN R

    IPC分类号： G01N33/50 ,  C07K14/705 ,  C07K14/71 ,  C07K14/72 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/85 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/53 ,  G01N33/543 ,  G01N33/554 ,  G01N33/566 ,  G01N33/567 ,  G01N33/68 ,  G01N33/92

    CPC分类号： G01N33/68 ,  C07K14/705 ,  C07K14/70571 ,  C07K14/71 ,  C07K14/723 ,  C07K2319/09 ,  C07K2319/60 ,  G01N33/5008 ,  G01N33/5035 ,  G01N33/5044 ,  G01N33/5058 ,  G01N33/6803 ,  G01N33/6863 ,  G01N33/92 ,  G01N2333/71 ,  G01N2333/72 ,  G01N2333/726 ,  G01N2458/00 ,  G01N2500/00

    摘要： PROBLEM TO BE SOLVED: To provide a method for screening a compound interacting with a transmembrane protein, and for determining whether a protein such as the transmembrane protein can form an oligomer or not. SOLUTION: In the method for screening a candidate compound interacting with at least one kind of transmembrane protein, first of all, a transmembrane protein containing at least one nuclear localization sequence (NLS) and a detectable moiety is expressed in a cell, and then, a candidate compound is brought into contact with the cell, and further, a distribution of the detectable moiety in the cell is detected, to thereby determine a distribution of an expressed protein in the cell. Concerning determination of whether a first protein and a second protein can form an oligomer or not, it can be determined whether the oligomer can be formed or not, by detecting the detectable moiety in a nucleus or near the nucleus of the cell, or by knowing occurrence of an interaction between the first protein and the second protein by detecting decline of a level of the detectable moiety on the cell surface, relative to a control cell. COPYRIGHT: (C)2010,JPO&INPIT

    摘要翻译： 要解决的问题：提供筛选与跨膜蛋白相互作用的化合物的方法，以及用于确定诸如跨膜蛋白之类的蛋白质是否可以形成低聚物。 解决方案：在用于筛选与至少一种跨膜蛋白相互作用的候选化合物的方法中，首先，在细胞中表达含有至少一个核定位序列（NLS）和可检测部分的跨膜蛋白， 然后使候选化合物与细胞接触，此外，检测细胞中可检测部分的分布，从而确定细胞中表达的蛋白质的分布。 关于第一种蛋白质和第二种蛋白质是否可以形成低聚物的判定，可以通过检测细胞核中或细胞核附近的可检测部分，或通过了解细胞核来确定是否可以形成寡聚体 相对于对照细胞，通过检测细胞表面上可检测部分的水平的下降，发生第一蛋白质和第二蛋白质之间的相互作用。 版权所有（C）2010，JPO＆INPIT

    High throughput cell-based assay, method of use and kit thereof
    87.
    发明专利
    High throughput cell-based assay, method of use and kit thereof 有权
    高通量电池测定方法及其使用方法

    公开(公告)号：JP2008200037A

    公开(公告)日：2008-09-04

    申请号：JP2008021013

    申请日：2008-01-31

    申请人： Millipore Corp ,  ミリポア・コーポレイションMillipore Corporation

    发明人： PARK JUN Y

    IPC分类号： C12Q1/02 ,  C12M1/34 ,  G01N33/543 ,  G01N33/554

    CPC分类号： G01N33/5005 ,  B01L3/50255 ,  B01L2300/0829 ,  B01L2400/0457 ,  G01N33/56966 ,  Y10T436/25125 ,  Y10T436/25375 ,  Y10T436/255

    摘要： PROBLEM TO BE SOLVED: To settle the problem that lack of high throughput and high cell survival rate/maintenance factor assay is a large bottleneck on maximization of drug discovery ability of proteomics. SOLUTION: Cells are placed in a multiwell plate and grown. When the assay is to be performed, gravity is used rather than vacuum or centrifugation to wash away any unbound ligands. The cells are then examined to detect the bound ligand. One or plural washing step(s) can be performed by placing the multiwell filter plate in a carrier plate having open wells matched with each well of the multiwell filter plate or using a wicking device or an underdrain, attached to the bottom of the multiwell filter plate. Sufficient wash liquid is added to allow for filtration by the effect of gravity to occur. Cells are retained within each well at a rate of 4 times that of other methods. COPYRIGHT: (C)2008,JPO&INPIT

    摘要翻译： 要解决的问题：解决缺乏高通量和高细胞存活率/维持因子测定的问题是蛋白质组学药物发现能力最大化的巨大瓶颈。

    解决方案：将细胞置于多孔板中并生长。 当进行测定时，使用重力而不是真空或离心以洗去任何未结合的配体。 然后检查细胞以检测结合的配体。 一个或多个洗涤步骤可以通过将多孔过滤板放置在具有与多孔过滤板的每个孔匹配的开口孔的载体板中，或者使用连接到多孔过滤器的底部的芯吸装置或暗渠， 盘子。 加入足够的洗涤液体以通过重力的作用进行过滤。 细胞以其他方法的4倍的速率保留在每个孔内。 版权所有（C）2008，JPO＆INPIT