公开(公告)号：JP5216072B2

公开(公告)日：2013-06-19

申请号：JP2010263148

申请日：2010-11-26

Applicant: 独立行政法人科学技術振興機構

Inventor： 和人 小林

IPC: C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P25/16 ,  C07K14/145 ,  C12N5/10 ,  C12N7/02

CPC classification number: C12N15/86 ,  A61K48/0058 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/03 ,  C12N2740/16045 ,  C12N2740/16071 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

Abstract: The purpose of the present invention is to provide a lentiviral vector system having a higher titer, while sustaining an excellent retrograde transport ability particularly in the brain. The present invention relates to a kit for preparing a retrograde transport viral vector comprising: (1) a packaging plasmid containing the gag gene and the pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing an target gene (a transgene); and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising a fused extracellular domain consisting of the N-terminal region of an extracellular domain of rabies virus glycoprotein (RV-G) and the C-terminal region of an extracellular domain of vesicular stomatitis virus glycoprotein (VSV-G), a transmembrane domain of RV-G orVSV-G, and an intracellular domain of VSV-G., and the like.

公开(公告)号：JP5123922B2

公开(公告)日：2013-01-23

申请号：JP2009274156

申请日：2009-12-02

Applicant: 独立行政法人科学技術振興機構

Inventor： 和人 小林

IPC: C12N15/09 ,  A61K35/76 ,  A61K48/00 ,  A61P25/16 ,  C07K14/145 ,  C12N5/10 ,  C12N7/02

CPC classification number: A61K48/0075 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

Abstract: Provided is a lentiviral vector system which sustains a high-frequency retrograde transportation ability in animal brain and has a higher titer. A kit for preparing a retrograde transport viral vector, which comprises: (1) a packaging plasmid containing the gag gene and pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing a target gene; and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising the extracellular domain of rabies virus glycoprotein (RV-G), the transmembrane domain of rabies virus glycoprotein (RV-G) or vesicular stomatitis virus glycoprotein (VSV-G) and the intracellular domain of vesicular stomatitis virus glycoprotein (VSV-G).