公开(公告)号：JP2013051973A

公开(公告)日：2013-03-21

申请号：JP2012274394

申请日：2012-12-17

Applicant: Dsm Ip Assets Bv ,  ディーエスエム アイピー アセッツ ビー.ブイ.

Inventor： REUCKER CRAIG M ,  DIMASI DON ,  HANSEN JON M ,  MIRRASOUL PETER J ,  BAILEY RICHARD B ,  VEEDER GEORGE T III ,  KANEKO TATSUO ,  BARCLAY WILLIAM R

IPC: C12N15/09 ,  C12P7/64 ,  A23B7/10 ,  A23D9/00 ,  A23L1/03 ,  C12N1/00 ,  C12N1/12 ,  C12N1/13 ,  C12R1/89

CPC classification number: A23K50/80 ,  A23D9/00 ,  A23K20/158 ,  A23L29/065 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/00 ,  C12N1/12 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  Y10S435/911

Abstract: PROBLEM TO BE SOLVED: To provide a method of proliferating eukaryotic microbes capable of producing a lipid, particularly a polyenoic fatty acid-containing lipid, and to provide a method of producing eukaryotic microbial lipids.SOLUTION: There are provided the method of proliferating the eukaryotic microorganisms capable of producing at least about 20% of an eukaryotic microorganisms biomass as lipids and the method of producing the lipids. The lipids preferably contain one or more polyenoic fatty acids. A carbon source, preferably a non-alcohol carbon source and a restricted nutrient source are added to a fermentation medium containing the eukaryotic microorganisms. The carbon source and the restricted nutrient source are preferably added at a rate sufficiently enough to increase the biomass density of the fermentation medium to at least about 100 g/L.

Abstract translation: 待解决的问题：提供能够产生脂质，特别是含多烯脂肪酸的脂质的真核微生物的增殖方法，并提供生产真核生物微生物脂质的方法。 解决方案：提供了能够产生至少约20％的作为脂质的真核微生物生物质的真核微生物的增殖方法和产生脂质的方法。 脂质优选含有一种或多种多烯酸脂肪酸。 将碳源，优选非醇碳源和限制性营养源加入到含有真核微生物的发酵培养基中。 碳源和受限营养源优选以足够足以将发酵培养基的生物量密度提高至至少约100g / L的速率加入。 版权所有（C）2013，JPO＆INPIT

公开(公告)号：JP2014087375A

公开(公告)日：2014-05-15

申请号：JP2014027674

申请日：2014-02-17

Applicant: Dsm Ip Assets Bv ,  ディーエスエム アイピー アセッツ ビー.ブイ.

Inventor： REUCKER CRAIG M ,  DIMASI DON ,  HANSEN JON M ,  MIRRASOUL PETER J ,  BAILEY RICHARD B ,  VEEDER GEORGE T III ,  KANEKO TATSUO ,  BARCLAY WILLIAM R

IPC: C12N15/09 ,  C12P7/64 ,  A23B7/10 ,  A23D9/00 ,  A23L1/03 ,  C12N1/00 ,  C12N1/12 ,  C12N1/13 ,  C12N1/14 ,  C12N1/15 ,  C12R1/645 ,  C12R1/89

CPC classification number: A23K50/80 ,  A23D9/00 ,  A23K20/158 ,  A23L29/065 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/00 ,  C12N1/12 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  Y10S435/911

Abstract: PROBLEM TO BE SOLVED: To provide a method for proliferating eukaryotic microorganisms which can produce lipids, in particular lipids containing polyenoic fatty acids, and a method for producing lipids of the eukaryotic microorganisms.SOLUTION: There are provided the method for proliferating eukaryotic microorganisms capable of producing at least about 20% of eukaryotic microorganism biomass as lipids, and the method for producing lipids of the same. The lipids preferably contain one or more polyenoic fatty acids, and a carbon source, preferably a non-alcohol carbon source, and restricted nutrient sources are added to a fermentation medium containing the eukaryotic microorganisms. The carbon source and the restricted nutrient sources are preferably added at a sufficient rate to increase biomass density of the fermentation medium at least at about 100 g/L.

Abstract translation: 要解决的问题：提供一种增殖能够产生脂质的真核微生物的方法，特别是含有多烯脂肪酸的脂质，以及用于生产真核微生物脂质的方法。提供了增殖真核微生物的方法，其能够 产生作为脂质的至少约20％的真核生物微生物，以及其生产脂质的方法。 脂质优选含有一种或多种多烯脂肪酸，并将碳源，优选非醇碳源和限制性营养源加入到含有真核微生物的发酵培养基中。 优选以足够的速率加入碳源和受限营养源，以将发酵培养基的生物质密度提高至至少约100g / L。

公开(公告)号：JP2013099366A

公开(公告)日：2013-05-23

申请号：JP2013041515

申请日：2013-03-04

Applicant: Dsm Ip Assets Bv ,  ディーエスエム アイピー アセッツ ビー.ブイ.

Inventor： REUCKER CRAIG M ,  DIMASI DON ,  HANSEN JON M ,  MIRRASOUL PETER J ,  BAILEY RICHARD B ,  VEEDER GEORGE T III ,  KANEKO TATSUO ,  BARCLAY WILLIAM R

IPC: C12N15/09 ,  C12P7/40 ,  A23B7/10 ,  A23D9/00 ,  A23L1/03 ,  C12N1/00 ,  C12N1/12 ,  C12N1/13 ,  C12P7/64 ,  C12R1/89

CPC classification number: A23K50/80 ,  A23D9/00 ,  A23K20/158 ,  A23L29/065 ,  A23V2002/00 ,  C11B1/10 ,  C12N1/00 ,  C12N1/12 ,  C12P7/6409 ,  C12P7/6427 ,  C12P7/6472 ,  Y10S435/911

Abstract: PROBLEM TO BE SOLVED: To provide a process for growing eukaryotic microorganisms which are capable of producing lipids, in particular lipids containing polyenoic fatty acids, and to provide a process for producing eukaryotic microbial lipids.SOLUTION: The present invention provides a process for growing eukaryotic microorganisms which are capable of producing at least about 20% of their biomass as lipids and a method for producing the lipids. Preferably the lipids contain one or more polyenoic fatty acids. The process comprises adding to a fermentation medium comprising eukaryotic microorganisms a carbon source, preferably a non-alcoholic carbon source, and a limited nutrient source. Preferably, the carbon source and the limited nutrient source are added at a rate sufficient to increase the biomass density of the fermentation medium to at least about 100 g/L.

Abstract translation: 待解决的问题：提供一种能够生产能够产生脂质的真核微生物的方法，特别是含有多烯脂肪酸的脂质，并提供一种生产真核微生物脂质的方法。 解决方案：本发明提供一种能够生产至少约20％的生物质作为脂质的真核微生物的方法和一种生产脂质的方法。 优选地，脂质含有一种或多种多烯脂肪酸。 该方法包括向包含真核微生物的发酵培养基中加入碳源，优选非酒精性碳源和有限营养源。 优选地，碳源和有限营养源以足以将发酵培养基的生物质密度增加至至少约100g / L的速率加入。 版权所有（C）2013，JPO＆INPIT