摘要:
PROBLEM TO BE SOLVED: To provide a method for directly extracting microbial or unicellular oils containing one or more polyunsaturated fatty acids (PUFAs) and the like from microbial cells without the need of a solvent.SOLUTION: After fermentation, microbial cells are pasteurised and washed, and the cell walls are lysed or disrupted by a mechanical (e.g. homogenization), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique. The oil (containing the PUFA) is then separated from the resulting cell wall debris. This is achieved by centrifugation, which results in an oily phase (top layer) that contains the oil. The oily phase is separated from an aqueous phase (containing the cell wall debris). The oil is then extracted and if necessary the PUFA is purified or isolated from the oil.
摘要:
PROBLEM TO BE SOLVED: To provide a process for treating an oil derived from microorganisms. SOLUTION: This process for treating an oil comprises bringing the oil into contact with a polar solvent to extract at least one compound that is soluble in the solvent, and then separating the solvent containing the compound from the so treated oil. The oil is microbially derived, and extracted either from a fermentation broth or a filtrate thereof using hexane. The compound to be extracted is usually a sterol or a diglyceride. The solvent is ethanol having up to 5% water. The oil can contain a polyunsaturated fatty acid such as 18C, 20C or 22C ω-3 or ω-6 fatty acid, such as arachidonic acid. COPYRIGHT: (C)2009,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a method for extracting (and subsequently isolating) an oil derived from microorganisms (or single cells), preferably an oil containing one or more kinds of polyunsaturated fatty acids (PUFA) from a single-cell organism (or a microbial cell), and an apparatus therefor.SOLUTION: The method comprises a step of pasteurizing the microbial cell after fermentation, washing the cell and lysing or disrupting the cell wall by a mechanical (e.g. homogenization), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique; a step to obtain an oily phase (upper layer) containing the oil by centrifugal separation; a step to separate the oily phase from an aqueous phase (containing cell wall debris) and separate the oil (containing PUFA) from the obtained cell wall debris; and a step to extract the oil and optionally purify or isolate the PUFA from the oil.
摘要:
PROBLEM TO BE SOLVED: To provide a microbial oil more stable than conventional polyunsaturated fatty acid-containing oils isolated from microbial biomass. SOLUTION: There is provided a method for obtaining an oil containing at least one polyunsaturated fatty acid from microbial biomass, which method comprises following (a)-(d). (a) A biomass having 25-80% dry matter content is supplied, (b) the biomass is granulated to be granules, (c) the granules are dried to be dried granules, and then, (d) the oil is extracted or isolated from the dried granules. COPYRIGHT: (C)2010,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a novel polyunsaturated fatty acid-containing microorganic oil and a method for recovering the same polyunsaturated fatty acid-containing microorganic oil.SOLUTION: (1) A polyunsaturated fatty acid (PUFA)-containing microorganic oil having a high triglyceride content and a high oxidation stability and (2) a method for recovering the same microorganic oil from microorganic biomass derived from a low-temperature fermentation broth are hereby provided; the provided method includes a step of forming granular particles by subjecting the biomass to extrusion and, after the granular particles have been dried, extracting the microorganic oil from the dried granular particles by using an appropriate solvent.
摘要:
PROBLEM TO BE SOLVED: To provide a new microbial polyunsaturated fatty acid containing oil, and to provide a method for the recovery of such microbial polyunsaturated fatty acid containing oil. SOLUTION: The present invention discloses (1) the microbial polyunsaturated fatty acid(PUFA)-containing oil with a high triglyceride content and a high oxidative stability, and (2) the method for the recovery of such oil from a microbial biomass derived from a pasteurised fermentation broth, wherein the microbial biomass is subjected to extrusion to form granular particles, dried and the oil then extracted from the dried granules using an appropriate solvent. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a new microorganism oil including polyunsaturated fatty acid and a method for recovering the same. SOLUTION: The method, which recovers (1) microorganism oil containing polyunsaturated fatty acid (PUFA) which has a high triglyceride content and high oxidation stability and (2) the microorganism oil from biomass induced from low-temperature fermentation broth, includes: the process of giving the biomass an extrusion treatment to form granule-like particles; the process of drying the particles; and, after that, the process of extracting the microorganism oil with suitable solvent. COPYRIGHT: (C)2011,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a microbial oil stable compared with a conventional polyunsaturated fatty acid containing oil isolated from a microbial biomass. SOLUTION: This method of preparing the oil containing the at least one kind of polyunsaturated fatty acid containing oil from the microbial biomass includes the following processes (a)-(d): (a) a process for supplying the biomass with 25-80% of dry content, (b) a process for granulating the biomass into a granule, (c) a process for drying the granule to obtain a dried granule, and (d) a process for extracting or separating the oil from the dried granules. COPYRIGHT: (C)2009,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a process of treating an oil derived from a microorganism.SOLUTION: A process of treating an oil is provided, the process comprising contacting the oil with a polar solvent to extract at least one compound that is soluble in the solvent, and then separating the solvent containing the compound from the treated oil. The oil is microbially derived, and extracted either from a fermentation broth or a filtrate thereof using hexane. The compound to be extracted is usually a sterol or a diglyceride. The solvent is ethanol having up to 5% water. The oil can contain a polyunsaturated fatty acid such as C18, C20 or C22 ω-3 or ω-6 fatty acid, such as arachidonic acid.
摘要:
PROBLEM TO BE SOLVED: To provide a process of treating a microbially derived oil.SOLUTION: The process of treating an oil comprises contacting the oil with a polar solvent to extract at least one compound that is soluble in the solvent, and then separating the solvent containing the compound from the so treated oil. The oil is microbially derived, and extracted either from a fermentation broth or a filtrate thereof using hexane. The compound to be extracted is usually a sterol or a diglyceride. The solvent is ethanol having up to 5% water. The oil can contain a polyunsaturated fatty acid such as C18, C20 or C22 ω-3 or ω-6 fatty acid, such as arachidonic acid.