公开(公告)号：US5641893A

公开(公告)日：1997-06-24

申请号：US605733

申请日：1996-02-22

申请人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

发明人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

IPC分类号： G01N30/52 ,  G01N30/60 ,  G01N30/62 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/7703 ,  G01N30/6069 ,  G01N30/62 ,  G01N2021/6484 ,  G01N2021/7786 ,  G01N2030/522 ,  G01N30/6021 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

摘要： An apparatus for the monitoring of a column chromatography separation process includes a segmented column with a seal positioned at the joint defined by the segments of the column. A connector is provided for connecting the segments of the column together. The apparatus further includes a sensor for monitoring an analyte in an eluant within a separation zone of the column. The sensor includes a mesh grid made of optical fibers or metal wires which is placed so as to extend through the separation zone of the column. The metal wires or optical fibers extend through the seal of the joint in the segmented column and connect to signal processing and data analysis equipment for purposes of monitoring the movements and concentration of an analyte in an eluant at various locations within the column. Certain segments of the optical fibers or metal wires which make up the mesh grid are coated so as to be desensitized and other segments are uncoated for sensing the analyte. This provides an effective apparatus to monitor in detail the cross-section of a column chromatography process in-situ.

摘要翻译： 用于监测柱色谱分离方法的装置包括分段柱，其具有位于由柱的段限定的接头处的密封。 提供连接器用于将柱的段连接在一起。 该装置还包括用于监测柱的分离区域内的洗脱液中的分析物的传感器。 传感器包括由光纤或金属线制成的网格格栅，该网格格栅被放置成延伸穿过柱的分离区。 金属线或光纤通过分段柱中的接头的密封件延伸，并连接到信号处理和数据分析设备，目的是监测柱内不同位置的洗脱液中分析物的运动和浓度。 构成网状网格的光纤或金属线的某些部分被涂覆以使其脱敏，并且其它部分未被涂覆以感测分析物。 这提供了一种现场详细监测柱色谱法的横截面的有效装置。