公开(公告)号：US12044663B2

公开(公告)日：2024-07-23

申请号：US16067628

申请日：2017-01-06

申请人： Cytiva BioProcess R&D AB

发明人： Klaus Gebauer ,  Dan Hermansson

IPC分类号： G01N30/60 ,  B01D15/20 ,  B01D15/22 ,  G01N30/56

CPC分类号： G01N30/6004 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N2030/565 ,  G01N30/6021 ,  G01N30/606

摘要： A method for packing a chromatography column with chromatography media, comprising the steps of: providing a system comprising a column tube (3,19,21) having a closed first end (5) comprising an inlet/outlet (7), a media inlet (15) adjacent a second end of the column tube and an adaptor (9) positioned inside the column tube initially adjacent the second end of the column tube for sliding and sealing contact with an inner face of the column tube, the column tube and adaptor arranged initially such that they define an internal volume and such that the media inlet is in fluid connection with the internal volume; connecting a media slurry source to the media inlet; at least partially filling the internal volume with media slurry via the media inlet; forcing the adaptor towards the first end of the column tube to reduce the internal volume such that the media inlet is no longer in fluid connection with the reduced internal volume.

公开(公告)号：US20240142414A1

公开(公告)日：2024-05-02

申请号：US18383828

申请日：2023-10-25

申请人： Bio-Rad Laboratories, Inc.

发明人： Christopher BELISLE ,  Mark A. SNYDER

IPC分类号： G01N30/52 ,  B01D15/22 ,  G01N30/60

CPC分类号： G01N30/52 ,  B01D15/22 ,  G01N30/6021 ,  G01N2030/522

摘要： A dynamic axial compression column is disclosed herein. This dynamic axial column utilized external compression to prevent the creation of end plate space in the column. The dynamic axial column can include a tube defining a first opening, a second opening, and a lumen extending there between. The dynamic axial column can include a first end plate assembly sealing the first opening and movably extending at least partially into the lumen via the first opening, a second end plate assembly sealing the second opening, a plurality of rods extending along the outside of the tube and connecting the first end plate assembly and the second end plate assembly, and a first plurality of compression devices external to the tube and engaging one of the plurality of rods to bias the first end plate assembly towards the second end plate assembly.

公开(公告)号：US20170097327A1

公开(公告)日：2017-04-06

申请号：US15379447

申请日：2016-12-14

申请人： GE Healthcare BioProcess R&D AB

发明人： KLAUS GEBAUER

IPC分类号： G01N30/60 ,  B01D15/22 ,  B01D15/18

CPC分类号： G01N30/6004 ,  B01D15/18 ,  B01D15/22 ,  G01N30/6017 ,  G01N30/6021 ,  G01N30/6052 ,  G01N30/606

摘要： The present invention relates to axial flow chromatography columns, methods for separating one or more analytes in a liquid by the use of such columns, and systems employing such columns. The column comprises a first port and a second port, the first port and said second port being at essentially the same level or elevation above the level of the bed space on the chromatography column.

公开(公告)号：US09327213B2

公开(公告)日：2016-05-03

申请号：US13771371

申请日：2013-02-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Sébastien Lefebvre ,  Lilian Portier ,  Maurice Agée

IPC分类号： B01D15/22 ,  B01D15/20 ,  B65B1/24 ,  G01N30/60 ,  F16L11/00 ,  F16L33/00 ,  B01D15/08

CPC分类号： B01D15/22 ,  B01D15/206 ,  B65B1/24 ,  F16L11/00 ,  F16L33/00 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/6082 ,  G01N2030/565 ,  Y10T29/49865

摘要： A chromatography column design and a packing device, as well as a method for obtaining a column with a very compact and uniform bed are provided. The bed remains compact and uniform during operation of the column and during sanitization, storage and transportation of the column. The amount of hardware, particularly the hardware parts in contact with product, is minimized, especially with the implementation of an internal liner. With this internal liner, the packed bed can be separated from the column in intact form, or even prepared separately from, and outside of, the column.

摘要翻译： 提供色谱柱设计和包装装置，以及用于获得具有非常紧凑和均匀的床的柱的方法。 在塔的运行期间和在柱的消毒，储存和运输期间，该床保持紧凑和均匀。 硬件的数量，特别是与产品接触的硬件部件的数量被最小化，特别是通过实施内衬。 使用这种内衬，填充床可以以完整的形式与柱分离，或者甚至与柱分离制备，也可以与柱分离。

公开(公告)号：US08632680B2

公开(公告)日：2014-01-21

申请号：US13575064

申请日：2011-01-26

申请人： Dan Hermansson ,  Girish Kittur ,  Patrik Akerstrom

发明人： Dan Hermansson ,  Girish Kittur ,  Patrik Akerstrom

IPC分类号： B01D15/08

CPC分类号： B01D15/22 ,  G01N30/6021 ,  G01N2030/565 ,  G01N2030/8813

摘要： Chromatography column comprising a column tube and at least one adjustable adapter assembly with a plunger that may be positioned at a plurality of longitudinal positions in the column tube, wherein the piston is arranged at the end of a threaded piston rod arranged with the threads engaged by a rotable end cap for enabling longitudinal movement of the piston by turning the end cap, and wherein the adaptor assembly comprises rotation prevention means arranged to prevent rotation of the plunger rod in response to turning of the end cap.

摘要翻译： 色谱柱包括柱管和至少一个可调整的适配器组件，其具有柱塞，柱塞可以定位在柱管中的多个纵向位置，其中活塞布置在螺纹活塞杆的端部，螺纹活塞杆的螺纹与 可旋转的端盖，用于通过转动端盖来实现活塞的纵向运动，并且其中适配器组件包括旋转防止装置，其被布置成防止柱塞杆响应于端盖的转动而旋转。

公开(公告)号：US20130062267A1

公开(公告)日：2013-03-14

申请号：US13672832

申请日：2012-11-09

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： Klaus Gebauer

IPC分类号： B01D15/20

CPC分类号： B01D15/206 ,  B01D15/14 ,  B01D15/22 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/606 ,  G01N2030/565

摘要： The invention relates to a method for providing an aseptic chromatography column, said method comprising the steps of: pre-sterilize an empty chromatography column; pre-sterilize a chromatography medium; introducing the pre-sterilized chromatography medium into the pre-sterilized chromatography column using aseptic equipment, thereby providing an aseptic chromatography column comprising chromatography medium.

摘要翻译： 本发明涉及一种提供无菌色谱柱的方法，所述方法包括以下步骤：对空色谱柱进行预消毒; 对色谱介质进行预灭菌; 使用无菌设备将预灭菌色谱介质引入预灭菌色谱柱，从而提供包含色谱介质的无菌色谱柱。

公开(公告)号：US20120292242A1

公开(公告)日：2012-11-22

申请号：US13575064

申请日：2011-01-26

申请人： Dan Hermansson ,  Girish Kittur ,  Patrik Akerstrom

发明人： Dan Hermansson ,  Girish Kittur ,  Patrik Akerstrom

IPC分类号： B01D15/10

CPC分类号： B01D15/22 ,  G01N30/6021 ,  G01N2030/565 ,  G01N2030/8813

摘要： Chromatography column comprising a column tube and at least one adjustable adapter assembly with a plunger that may be positioned at a plurality of longitudinal positions in the column tube, wherein the piston is arranged at the end of a threaded piston rod arranged with the threads engaged by a rotable end cap for enabling longitudinal movement of the piston by turning the end cap, and wherein the adaptor assembly comprises rotation prevention means arranged to prevent rotation of the plunger rod in response to turning of the end cap.

摘要翻译： 色谱柱包括柱管和至少一个可调整的适配器组件，其具有柱塞，柱塞可以定位在柱管中的多个纵向位置，其中活塞布置在螺纹活塞杆的端部，螺纹活塞杆的螺纹与 可旋转的端盖，用于通过转动端盖来实现活塞的纵向运动，并且其中适配器组件包括旋转防止装置，其被布置成防止柱塞杆响应于端盖的转动而旋转。

公开(公告)号：US08137549B2

公开(公告)日：2012-03-20

申请号：US12173906

申请日：2008-07-16

申请人： Torvald Andersson ,  Mats Olsson ,  Bengt Asberg ,  Lars Andersson

发明人： Torvald Andersson ,  Mats Olsson ,  Bengt Asberg ,  Lars Andersson

IPC分类号： B01D15/08

CPC分类号： B01D15/206 ,  B01D15/20 ,  G01N30/56 ,  G01N30/6021 ,  G01N2030/562 ,  G01N2030/8804

摘要： A column packing system comprises a control unit provided with software for calculating the breakpoint where a movable adapter comes into contact with a settled bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

摘要翻译： 列填充系统包括控制单元，该控制单元设置有用于计算可移动适配器与沉积床层介质接触的断点的软件。 所计算的断点由控制单元用于确定可移动适配器进一步移动到柱中以进一步达到期望的床压缩量。

公开(公告)号：US20100276371A1

公开(公告)日：2010-11-04

申请号：US12839497

申请日：2010-07-20

申请人： JOHN DAVIS ,  STEFAN K. ERIKSSON

发明人： JOHN DAVIS ,  STEFAN K. ERIKSSON

IPC分类号： B01D15/08

CPC分类号： B01D15/10 ,  B01D15/206 ,  B01D15/22 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/6047 ,  Y10T29/49719 ,  Y10T29/49721

摘要： A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device.

摘要翻译： 描述了色谱柱和维护方法，其不需要使用提升机或起重机来拆卸。 该方法通过减少操作者在柱内悬挂或支撑的负载下方的工作的需要来提供改进的操作员安全性。 此外，通过提供对塔的内部的通道和通过提供处理装置来促进塔部件的移除或更换。

公开(公告)号：US07807054B2

公开(公告)日：2010-10-05

申请号：US12313332

申请日：2008-11-19

申请人： John Graham Dunkley ,  Neil Francis Frazer ,  Melvyn John Gill

发明人： John Graham Dunkley ,  Neil Francis Frazer ,  Melvyn John Gill

IPC分类号： B01D15/08

CPC分类号： G01N30/56 ,  B01D15/20 ,  B01D15/22 ,  G01N30/6021 ,  G01N30/6047 ,  G01N30/6082 ,  G01N2030/522 ,  G01N2030/562 ,  Y10T29/49721 ,  G01N30/6004

摘要： The present invention relates to a chromatography column that removes the longitudinal load from the column tube altogether and transfers it to a yoke and stanchion arrangement situated around but external to the column tube. By using the yoke/stanchion system, the central adjuster can be used to move the top end into and out of the housing. Thus, when internal pressure is applied to the column, the tube experiences no longitudinal load. Additionally, the yoke and stanchion design allows for complete removal of the end from the tube without cumbersome disassembly. Moreover, the yoke provides a place to retain the end while the tube is being filled, emptied, cleaned or repaired.

摘要翻译： 本发明涉及一种色谱柱，其色谱柱一直从柱管中除去纵向载荷，并将其转移到位于柱管周围但位于柱管周围的轭和支柱装置。 通过使用轭/支柱系统，中央调节器可用于将顶端移入和移出壳体。 因此，当向柱施加内部压力时，管不经受纵向载荷。 另外，轭和支柱设计允许从管中完全移除端部而不需要麻烦的拆卸。 此外，轭提供了在管被填充，清空，清洁或修理时保持端部的位置。