公开(公告)号：US5389523A

公开(公告)日：1995-02-14

申请号：US917426

申请日：1992-07-23

Applicant: Anne L. Plant ,  Laurie Locascio-Brown ,  Richard A. Durst

Inventor： Anne L. Plant ,  Laurie Locascio-Brown ,  Richard A. Durst

IPC: G01N33/543 ,  G01N35/08 ,  G01N33/544 ,  G01N33/547

CPC classification number: G01N35/085 ,  G01N33/5432 ,  Y10S435/81

Abstract: A method of immunoanalysis combines immobilized immunochemistry with the technique of flow injection analysis, and employs microscopic spherical structures called liposomes, or lipid vesicles, as carriers of detectable reagents. Liposomes are modified on their surface with analytical reagents, and carry in their internal volume a very large number of fluorescent or electroactive molecules. Aspects of this embodiment of the invention include the chemistry for covalent immobilization of antibody fragments in a specified orientation, the use of liposomes in a flow injection analysis system, and the combination of automated sampling and analysis with reusable immunoreactants. Another aspect of the invention involves the non-covalent binding of liposomes to a receptor for use in a homogeneous assay. In another aspect of the invention the intensity of scattered light is quantitated as a measure of liposome aggregation in response to a concentration-dependent immunospecific reaction.

Abstract translation: 免疫分析方法将固定化免疫化学与流动注射分析技术相结合，并使用称为脂质体或脂质囊泡的微观球形结构作为可检测试剂的载体。 脂质体在其表面上用分析试剂修饰，并在其内部体积中携带非常大量的荧光或电活性分子。 本发明该实施方案的方面包括以特定取向共价固定抗体片段的化学，在流动注射分析系统中使用脂质体，以及将自动取样和分析与可重复使用的免疫反应物的组合。 本发明的另一方面涉及脂质体与受体的非共价结合，用于均一测定。 在本发明的另一方面，响应于浓度依赖性免疫特异性反应，散射光的强度被定量为脂质体聚集的量度。

公开(公告)号：US5641893A

公开(公告)日：1997-06-24

申请号：US605733

申请日：1996-02-22

Applicant: Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

Inventor： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

IPC: G01N30/52 ,  G01N30/60 ,  G01N30/62 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

CPC classification number: G01N21/648 ,  G01N21/6428 ,  G01N21/7703 ,  G01N30/6069 ,  G01N30/62 ,  G01N2021/6484 ,  G01N2021/7786 ,  G01N2030/522 ,  G01N30/6021 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

Abstract: An apparatus for the monitoring of a column chromatography separation process includes a segmented column with a seal positioned at the joint defined by the segments of the column. A connector is provided for connecting the segments of the column together. The apparatus further includes a sensor for monitoring an analyte in an eluant within a separation zone of the column. The sensor includes a mesh grid made of optical fibers or metal wires which is placed so as to extend through the separation zone of the column. The metal wires or optical fibers extend through the seal of the joint in the segmented column and connect to signal processing and data analysis equipment for purposes of monitoring the movements and concentration of an analyte in an eluant at various locations within the column. Certain segments of the optical fibers or metal wires which make up the mesh grid are coated so as to be desensitized and other segments are uncoated for sensing the analyte. This provides an effective apparatus to monitor in detail the cross-section of a column chromatography process in-situ.

Abstract translation: 用于监测柱色谱分离方法的装置包括分段柱，其具有位于由柱的段限定的接头处的密封。 提供连接器用于将柱的段连接在一起。 该装置还包括用于监测柱的分离区域内的洗脱液中的分析物的传感器。 传感器包括由光纤或金属线制成的网格格栅，该网格格栅被放置成延伸穿过柱的分离区。 金属线或光纤通过分段柱中的接头的密封件延伸，并连接到信号处理和数据分析设备，目的是监测柱内不同位置的洗脱液中分析物的运动和浓度。 构成网状网格的光纤或金属线的某些部分被涂覆以使其脱敏，并且其它部分未被涂覆以感测分析物。 这提供了一种现场详细监测柱色谱法的横截面的有效装置。