公开(公告)号：US11946911B2

公开(公告)日：2024-04-02

申请号：US17049249

申请日：2019-01-25

申请人： SHIMADZU CORPORATION

发明人： Yanli Guo ,  Naoki Hamada ,  Yuki Hashi

IPC分类号： G01N30/46 ,  G01N30/06 ,  G01N30/88 ,  G01N30/89

CPC分类号： G01N30/06 ,  G01N30/46 ,  G01N30/88 ,  G01N2030/065 ,  G01N30/89

摘要： The present invention relates to an analysis system that is applied to rapid automatic analysis and detection of highly lipophilic components and a method for rapidly detecting vitamin D in an oil or fat, or a biological sample. In the present invention, online pretreatment and separation of a sample containing highly lipophilic components in a sample to be analyzed are performed by a multidimensional chromatography system. The multidimensional chromatography system includes a supercritical chromatography part and a reverse phase liquid chromatography part sequentially connected, the reverse phase liquid chromatography part including one or more reverse phase liquid chromatography columns, and the supercritical chromatography part including a supercritical mobile phase, a modifier, and a supercritical packed column.

公开(公告)号：US10077404B2

公开(公告)日：2018-09-18

申请号：US15532426

申请日：2015-12-01

申请人： W. R. Grace & Co.-Conn.

发明人： Dieter Wallenstein ,  Stefan Brandt

IPC分类号： C10G11/18 ,  G01N13/00 ,  G01N15/08 ,  G01N30/89

CPC分类号： C10G11/187 ,  C10G2300/708 ,  G01N13/00 ,  G01N15/082 ,  G01N30/89 ,  G01N2013/003

摘要： Process for evaluating the catalytic performance of a porous solid using a vapor diffusion technique, where a probe molecule and a molecule for dead-time determination is injected into a carrier gas that is then contacted with the porous solid in a vessel, where a detector analyzes the peak width and retention time of a probe molecule and the retention time of the molecule for dead-time determination in the gas exiting the vessel.

公开(公告)号：US20050193802A1

公开(公告)日：2005-09-08

申请号：US11072829

申请日：2005-03-04

申请人： Andrew Tipler

发明人： Andrew Tipler

IPC分类号： B01D46/46 ,  G01B13/00 ,  G01B13/08 ,  G01N15/08 ,  G01N30/08 ,  G01N30/32 ,  G01N30/52 ,  G01N30/54 ,  G01N30/60 ,  G01N30/62 ,  G01N30/86 ,  G01N30/89

CPC分类号： G01N30/89 ,  G01N15/0826 ,  G01N30/32 ,  G01N30/60 ,  G01N2030/324 ,  G01N2030/524 ,  G01N2030/626 ,  G01N2030/525

摘要： Disclosed are systems and methods that include providing a vessel having an adsorbent disposed therein, the vessel having an inlet and an outlet for communicating a carrier gas through the vessel; determining a differential pressure between the inlet and the outlet for a known flow rate at the outlet; and, determining a geometric measure for the vessel based on a ratio of the known flow rate and the differential pressure. In some embodiments, the methods and systems can include a viscosity in the ratio, determining a flow rate at ambient pressure using a factor based on a ratio of a gas pressure at the output and ambient pressure, and/or, determining a flow rate at ambient temperature using a factor based on a ratio of a temperature at the output and ambient temperature.

摘要翻译： 公开了包括提供其中设置有吸附剂的容器的系统和方法，所述容器具有用于使载气通过容器连通的入口和出口; 确定出口处的已知流速的入口和出口之间的压差; 并且基于已知流量和压差的比率确定容器的几何度量。 在一些实施方案中，方法和系统可以包括该比例的粘度，使用基于输出处的气体压力和环境压力的比率的因子确定环境压力下的流速，和/或确定 使用基于输出温度和环境温度的比率的因子的环境温度。

公开(公告)号：US20050106740A1

公开(公告)日：2005-05-19

申请号：US10713761

申请日：2003-11-13

申请人： Barry Boyes ,  Nina Zolotarjova ,  Gordon Nicol

发明人： Barry Boyes ,  Nina Zolotarjova ,  Gordon Nicol

IPC分类号： C07K1/22 ,  C07K1/36 ,  G01N27/447 ,  G01N30/46 ,  G01N30/89 ,  G01N33/543 ,  G01N33/68 ,  G01N30/02

CPC分类号： G01N33/543 ,  C07K1/22 ,  C07K1/36 ,  G01N27/44726 ,  G01N27/44747 ,  G01N27/44773 ,  G01N30/461 ,  B01D15/3804

摘要： Methods, systems and device for performing analytical protocols are provided. Embodiments of the subject methods include sequentially contacting a sample with at least a first stationary phase and a second stationary phase under chromatographic conditions to at least determine the binding identity of the at least one constituent, where the specificity of the first stationary phase for at least one constituent present in the sample is at least uncertain and the specificity of the second stationary phase for the at least one constituent is certain. Also provided are systems and devices that include at least the first and second stationary phases. Kits for use in practicing the subject methods are also provided.

摘要翻译： 提供了用于执行分析方案的方法，系统和设备。 本发明方法的实施方案包括在色谱条件下使样品与至少第一固定相和第二固定相顺序接触以至少确定至少一种成分的结合同一性，其中第一固定相的特异性至少为 样品中存在的一个成分至少是不确定的，并且对于至少一种成分的第二固定相的特异性是确定的。 还提供了至少包括第一和第二固定相的系统和装置。 还提供了用于实践主题方法的套件。

公开(公告)号：US5641893A

公开(公告)日：1997-06-24

申请号：US605733

申请日：1996-02-22

申请人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

发明人： Lynn S. Penn ,  Donna L. Scott ,  Howard H. Weetall ,  Anne L. Plant ,  Marion F. McCurley ,  Scott A. Glazier

IPC分类号： G01N30/52 ,  G01N30/60 ,  G01N30/62 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/7703 ,  G01N30/6069 ,  G01N30/62 ,  G01N2021/6484 ,  G01N2021/7786 ,  G01N2030/522 ,  G01N30/6021 ,  G01N30/6034 ,  G01N30/6082 ,  G01N30/64 ,  G01N30/74 ,  G01N30/89

摘要： An apparatus for the monitoring of a column chromatography separation process includes a segmented column with a seal positioned at the joint defined by the segments of the column. A connector is provided for connecting the segments of the column together. The apparatus further includes a sensor for monitoring an analyte in an eluant within a separation zone of the column. The sensor includes a mesh grid made of optical fibers or metal wires which is placed so as to extend through the separation zone of the column. The metal wires or optical fibers extend through the seal of the joint in the segmented column and connect to signal processing and data analysis equipment for purposes of monitoring the movements and concentration of an analyte in an eluant at various locations within the column. Certain segments of the optical fibers or metal wires which make up the mesh grid are coated so as to be desensitized and other segments are uncoated for sensing the analyte. This provides an effective apparatus to monitor in detail the cross-section of a column chromatography process in-situ.

摘要翻译： 用于监测柱色谱分离方法的装置包括分段柱，其具有位于由柱的段限定的接头处的密封。 提供连接器用于将柱的段连接在一起。 该装置还包括用于监测柱的分离区域内的洗脱液中的分析物的传感器。 传感器包括由光纤或金属线制成的网格格栅，该网格格栅被放置成延伸穿过柱的分离区。 金属线或光纤通过分段柱中的接头的密封件延伸，并连接到信号处理和数据分析设备，目的是监测柱内不同位置的洗脱液中分析物的运动和浓度。 构成网状网格的光纤或金属线的某些部分被涂覆以使其脱敏，并且其它部分未被涂覆以感测分析物。 这提供了一种现场详细监测柱色谱法的横截面的有效装置。

公开(公告)号：US20240183826A9

公开(公告)日：2024-06-06

申请号：US17915834

申请日：2021-04-01

申请人： Chugai Seiyaku Kabushiki Kaisha

发明人： Motohiko Sato ,  Hiroko Murata

IPC分类号： G01N30/02 ,  G01N30/89

CPC分类号： G01N30/02 ,  G01N30/89

摘要： The present disclosure provides an analysis method for measuring the content of light chain-exchanged molecules in a composition containing a multi-specific antigen-binding molecule. The analysis method of the present disclosure includes the steps of: treating a composition comprising a multi-specific antigen-binding molecule and preparing a plurality of types of F(ab) fragments; and measuring the F(ab) fragments by a separation method based on electric charge or hydrophobic interactions and determining the content (content ratio) of each fragment.

公开(公告)号：US20200326315A1

公开(公告)日：2020-10-15

申请号：US16089503

申请日：2017-01-05

申请人： SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA

发明人： Ruijun Tian ,  Wendong Chen

IPC分类号： G01N30/06 ,  G01N30/89 ,  G01N30/60

摘要： Disclosed is a proteomic reactor, comprising a pipette tip, an ion exchange resin filler and a solid-phase extraction membrane. The solid-phase extraction membrane is filled into the lower end of the pipette tip, and the ion exchange resin filler is filled into the lower end of the pipette tip and is located above the solid-phase extraction membrane. The ion exchange resin filler is a strong cation exchange resin filler or a strong anion exchange resin filler. Disclosed is a protein chromatographic separation platform comprising the proteomic reactor and a liquid chromatography-mass spectrometer. Disclosed is the use of the proteomic reactor and protein chromatographic separation platform in the protein identification and protein quantitative analysis of a cell, a tissue or a blood sample.

公开(公告)号：US20190195840A1

公开(公告)日：2019-06-27

申请号：US15952367

申请日：2018-04-13

申请人： TAIHO PHARMACEUTICAL CO., LTD.

发明人： Yoshinori TOKIMITSU

IPC分类号： B01J20/281 ,  C07H19/06 ,  G01N30/89

CPC分类号： G01N30/48 ,  C07H19/06 ,  G01N30/89 ,  G01N2030/027 ,  G01N2030/486

摘要： This invention provides a method that is capable of detecting a trifluridine-related substance and a tipiracil-related substance contained in a sample containing trifluridine or a salt thereof and tipiracil or a salt thereof using the same procedure. The method is for detecting a trifluridine-related substance or a tipiracil-related substance or both, the method comprising the step of subjecting a sample containing trifluridine or a salt thereof and tipiracil or a salt thereof to high-performance liquid chromatography using a mobile phase composed of an organic phase and an aqueous phase.

公开(公告)号：US20050210957A1

公开(公告)日：2005-09-29

申请号：US11072371

申请日：2005-03-04

申请人： Andrew Tipler ,  Neil Plant

发明人： Andrew Tipler ,  Neil Plant

IPC分类号： B01D46/46 ,  G01B13/00 ,  G01B13/08 ,  G01N15/08 ,  G01N30/08 ,  G01N30/32 ,  G01N30/52 ,  G01N30/54 ,  G01N30/60 ,  G01N30/62 ,  G01N30/86 ,  G01N30/89

CPC分类号： G01N30/89 ,  G01N15/0826 ,  G01N30/32 ,  G01N30/60 ,  G01N2030/324 ,  G01N2030/524 ,  G01N2030/626 ,  G01N2030/525

摘要： Disclosed are systems and methods that include providing a vessel having an adsorbent disposed therein, the vessel having an inlet and an outlet for communicating a carrier gas through the vessel; determining a differential pressure between the inlet and the outlet for a known flow rate at the outlet; and, determining a geometric measure for the vessel based on a ratio of the known flow rate and the differential pressure. In some embodiments, the methods and systems can include a viscosity in the ratio, determining a flow rate at ambient pressure using a factor based on a ratio of a gas pressure at the output and ambient pressure, and/or, determining a flow rate at ambient temperature using a factor based on a ratio of a temperature at the output and ambient temperature.

公开(公告)号：US6139735A

公开(公告)日：2000-10-31

申请号：US255881

申请日：1999-02-23

申请人： Irving Wainer ,  Yanxiao Zhang

发明人： Irving Wainer ,  Yanxiao Zhang

IPC分类号： B01J20/281 ,  B01D15/08 ,  B01J20/28 ,  B01J20/32 ,  C12Q1/02 ,  G01N30/02 ,  G01N30/52 ,  G01N30/72 ,  G01N30/88 ,  G01N30/89

CPC分类号： B01D15/3804 ,  B01J20/28033 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3246 ,  B01J20/3251 ,  B01J20/3274 ,  G01N30/48 ,  B01J2220/54 ,  B01J2220/58 ,  G01N2030/527 ,  G01N30/02 ,  G01N30/7233 ,  G01N30/89

摘要： This invention provides a immobilized receptors on supports in liquid chromatographic systems. The method of the invention provides means of evaluating the attachment of agents to receptors comprising the steps of:(a) immobilizing receptors on artificial membrane supports in a column,(b) exposing the supports with the receptors to test agents at varying concentrations in a liquid chromatographic system,(c) eluting the test agent from the column, and(d) evaluating the elution profile of the test materials from the column.Using this method, it is possible to evaluate the interaction of the test agent with the receptor. Following elution, it is possible to directly determine molecular structure by passing the elute through other testing devices such as a mass spectrometer.