公开(公告)号：US09880158B2

公开(公告)日：2018-01-30

申请号：US13376103

申请日：2010-06-02

申请人： Jacobus Johannes Maria Van Dongen ,  José Alberto Orfao De Matos Correia E Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes Van Der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary De Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

发明人： Jacobus Johannes Maria Van Dongen ,  José Alberto Orfao De Matos Correia E Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes Van Der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary De Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N15/14 ,  G01N33/50

CPC分类号： G01N33/5094 ,  G01N15/14 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/532 ,  G01N33/533 ,  G01N2015/1402 ,  G01N2015/1413

摘要： The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

公开(公告)号：US20120165213A1

公开(公告)日：2012-06-28

申请号：US13376103

申请日：2010-06-02

申请人： Jacobus Johannes Maria van Dongen ,  José Alberto Orfao de Matos Correia e Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes van der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary de Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

发明人： Jacobus Johannes Maria van Dongen ,  José Alberto Orfao de Matos Correia e Vale ,  Juan Alejandro Flores-Montero ,  Julia Maria Almeida Parra ,  Vincent Henricus Johannes van der Velden ,  Sebastian Böttcher ,  Andrew Craig Rawstron ,  Ruth Mary de Tute ,  Ludovic Bernard Simon Lhermitte ,  Vahid Asnafi ,  Ester Mejstríková ,  Tomasz Szczepanski ,  Paulo Jorge Monteiro Da Silva Lucio ,  Marta Marín Ayuso ,  Carlos Eduardo Pedreira

IPC分类号： C40B30/04 ,  C40B40/10

CPC分类号： G01N33/5094 ,  G01N15/14 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/532 ,  G01N33/533 ,  G01N2015/1402 ,  G01N2015/1413

摘要： The invention relates to the field of flow cytometry and more particularly to a panel of antibody reagents conjugated to fluorescent compounds. Provided are reagent compositions, comprising at least eight distinct fluorochrome-conjugated antibodies comprising a set of at least three identification antibodies for the identification of a leukocyte population of interest and at least four characterization antibodies for further characterization and/or classification of said leukocyte population. Also provided are kits and methods related to the reagent compositions.

摘要翻译： 本发明涉及流式细胞术领域，更具体地涉及与荧光化合物缀合的一组抗体试剂。 提供了试剂组合物，其包含至少八种不同的荧光染料缀合的抗体，其包含一组至少三种用于鉴定目的白细胞群的鉴定抗体和用于进一步表征和/或分类所述白细胞群体的至少四种表征抗体。 还提供了与试剂组合物相关的试剂盒和方法。

公开(公告)号：US06686165B2

公开(公告)日：2004-02-03

申请号：US09752635

申请日：2000-12-29

申请人： Jacobus Johannes Maria van Dongen ,  Vincent Henricus Johannes van der Velden ,  Paulus Benjamin Berendes ,  Adriana Cornelia van Denderen

发明人： Jacobus Johannes Maria van Dongen ,  Vincent Henricus Johannes van der Velden ,  Paulus Benjamin Berendes ,  Adriana Cornelia van Denderen

IPC分类号： G01N3353

CPC分类号： G01N33/57496 ,  G01N33/537 ,  G01N33/57426 ,  G01N2333/82

摘要： The invention relates to the field of cancer diagnosis and the application of diagnostic techniques in pathology and hematology. Specifically, the invention relates to flow cytometric techniques for the detection of chromosomal aberrations and the detection of tumor specific gene products exclusively expressed by tumor cells containing said chromosomal aberrations. The invention provides a method to detect chromosomal aberrations in a biological sample via the exclusive detection of tumor-specific gene-product using at least two different probes directed against the gene-product.

摘要翻译： 本发明涉及癌症诊断领域和诊断技术在病理学和血液学中的应用。 具体地，本发明涉及用于检测染色体畸变的流式细胞术技术和仅含有所述染色体畸变的肿瘤细胞表达的肿瘤特异性基因产物的检测。 本发明提供了一种通过使用针对基因产物的至少两种不同探针来独占检测肿瘤特异性基因产物来检测生物样品中染色体畸变的方法。

公开(公告)号：US4971925A

公开(公告)日：1990-11-20

申请号：US142763

申请日：1988-01-11

申请人： Elizabeth M. L. Alexander ,  Jan Haisma ,  Theodorus Michielsen ,  Johannes Van Der Velden ,  Johannes F. C. M. Verhoeven

发明人： Elizabeth M. L. Alexander ,  Jan Haisma ,  Theodorus Michielsen ,  Johannes Van Der Velden ,  Johannes F. C. M. Verhoeven

IPC分类号： H01L21/304 ,  H01L21/31 ,  H01L21/762 ,  H01L27/00

CPC分类号： H01L21/76297 ,  Y10S148/135 ,  Y10S148/164

摘要： In a method of manufacturing a semiconductor device of the "semiconductor on insulator" type comprising at least one carrier body and a monocrystalline semiconductor body, in a major surface (2) of a monocrystalline semiconductor body (1) grooves (3) are provided having a predetermined depth. The surface provided with grooves is coated with a layer (4) of material resistant to polishing; and this layer is coated with a layer (5) of a chemomechanically polishable material having a layer thickness exceeding the groove depth, the latter layer (5) being polished to flatness and smoothness. The polished surface of the semiconductor body (1) is connected to a smooth flat major surface of a carrier body (6). Subsequently, the semiconductor body (1) is made thin, at least the last part of this operation consisting of a polishing step, which terminates on the layer (4) of material resistant to polishing so that mutually insulated "semiconductor an insulator" regions are obtained, the thickness of the semiconductor regions being equal to the depth of the grooves.