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    Cell-based methods for coupling protein interactions and binding molecule selection
    1.
    发明授权
    Cell-based methods for coupling protein interactions and binding molecule selection 有权
    用于偶联蛋白质相互作用和结合分子选择的基于细胞的方法

    公开(公告)号：US09487773B2

    公开(公告)日：2016-11-08

    申请号：US14193679

    申请日：2014-02-28

    Applicant: TECHNOPHAGE, INVESTIGAçÂO E DESENVOLVIMENTO EM BIOTECNOLOGIA, SA

    Inventor： João Manuel Braz Gonçalves ,  Frederico Nuno Castanheira Aires da Silva ,  Sofia Volker Côrte-Real ,  Soraia Rafaela Santiago de Oliveira

    IPC: C12N15/10 ,  G01N33/537 ,  G01N33/566

    CPC classification number: C12N15/1037 ,  C12N15/1024 ,  C12N15/1055 ,  G01N33/537 ,  G01N33/566 ,  G01N2500/02 ,  G01N2500/10

    Abstract: The invention relates to cell-based methods for diversifying, expressing and selecting binding molecules, e.g., antibodies, and target molecules to which they bind, all of which are expressed in the same cell. The target molecule can be a member of a ligand binding pair comprising a cell-surface expressed ligand binding receptor molecule and its cognate ligand, which interact within the cell. The methods provide retaining either the antibody or its target in a cell organelle as the site of binding and interaction. By performance of the methods, the binding or non-binding of the antibody to its target molecule within the cell produces a cell phenotype that is detectable at the cell surface via high throughput assays, e.g., flow cytometry. The methods are particularly useful for generating, recovering and providing antibodies that have optimal target molecule binding properties or activities for potential therapeutic use. Methods for generating diversity in such antibodies are also provided.

    Abstract translation: 本发明涉及用于多样化，表达和选择结合分子例如抗体和它们结合的靶分子的基于细胞的方法，所有这些分子在相同的细胞中表达。 靶分子可以是配体结合对的成员，其包含在细胞内相互作用的细胞表面表达的配体结合受体分子及其同源配体。 该方法提供将抗体或其靶标保留在细胞器中作为结合和相互作用的位点。 通过该方法的实施，抗体与细胞内的靶分子的结合或非结合产生可通过高通量测定例如流式细胞术在细胞表面检测的细胞表型。 该方法对于产生，回收和提供具有最佳靶分子结合特性或潜在治疗用途活性的抗体特别有用。 还提供了在这种抗体中产生多样性的方法。

    Detection of specific antigens in a population of antigens
    6.
    发明授权
    Detection of specific antigens in a population of antigens 有权
    检测抗原群体中的特异性抗原

    公开(公告)号：US09182389B2

    公开(公告)日：2015-11-10

    申请号：US13385428

    申请日：2012-02-21

    Applicant: Henry A. Graham ,  John G. Gorman ,  James P. Rowell

    Inventor： Henry A. Graham ,  John G. Gorman ,  James P. Rowell

    IPC: G01N33/541 ,  G01N33/537 ,  G01N33/80 ,  G01N33/53 ,  G01N33/543

    CPC classification number: G01N33/56966 ,  G01N33/5304 ,  G01N33/537 ,  G01N33/541 ,  G01N33/54326 ,  G01N33/80 ,  G01N2333/705 ,  G01N2446/00

    Abstract: Detection of the presence or absence of, RH positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The novel methods utilize gravitational forces or applying magnetic forces to reactive magnetic particles to isolate, distinguish and quantify cells.Particles aggregate and are pulled or settle by gravity through a transparent separating solution into a measuring zone where the volume of Rh positive cells can be measured. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.

    Abstract translation: 如在胎儿母体出血（FMH）中发现的，在Rh阴性细胞的混合群体中检测RH阳性细胞的存在或不存在。 新颖的方法利用重力或对反应性磁性颗粒施加磁力来分离，区分和定量细胞。 颗粒聚集并通过重力通过透明分离溶液拉伸或沉降到可以测量Rh阳性细胞体积的测量区域。 Rh阳性细胞体积与原始血液样品的体积相关，作为需要施用于母亲以防止随后的Rh免疫的RhIG剂量数量的指示。

    MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS
    7.
    发明申请
    MICROFLUIDIC DEVICES FOR THE CAPTURE OF BIOLOGICAL SAMPLE COMPONENTS 有权
    用于捕获生物样品组分的微流体装置

    公开(公告)号：US20150285809A1

    公开(公告)日：2015-10-08

    申请号：US14684104

    申请日：2015-04-10

    Applicant: The General Hospital Corporation

    Inventor： Thomas A. Barber ,  Ajay Shah ,  John Walsh ,  Mehmet Toner ,  Ravi Kapur ,  Shannon Stott

    IPC: G01N33/574 ,  G01N33/543 ,  G01N33/569

    CPC classification number: G01N33/57492 ,  B01L3/502753 ,  B01L2300/0816 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N15/1484 ,  G01N33/537 ,  G01N33/54306 ,  G01N33/56972

    Abstract: Methods and systems for selectively capturing analytes, such as cells, e.g., circulating tumor cells (CTCs), from fluid samples are disclosed. The methods include contacting the sample with an analyte binding moiety that selectively binds to the analytes; optionally separating first components of the sample including a majority of the analytes bound to the binding moieties from second components of the sample using size-based separation, e.g., in a microfluidic channel; adding to the first components of the sample a plurality of binding agents under conditions that enable a plurality of the binding agents to be linked to the analyte binding moieties to form multivalent tagging agents bound to the analyte; passing the first components of the sample past a surface to which is attached a plurality of capture agents that selectively bind to the binding agents; and capturing the analytes by providing conditions that enable the multivalent tagging agents bound to the analytes to bind to one or more of the capture agents.

    Abstract translation: 公开了用于从流体样品中选择性地捕获分析物例如细胞例如循环肿瘤细胞（CTC）的方法和系统。 所述方法包括使样品与选择性结合分析物的分析物结合部分接触; 任选地使用基于尺寸的分离（例如在微流体通道中）分离样品的第一组分，其包含与样品的第二组分结合至结合部分的大部分分析物; 在允许多个结合剂连接到分析物结合部分以形成与分析物结合的多价标签试剂的条件下，向样品的第一组分加入多种结合剂; 使样品的第一组分通过附着有选择性结合粘合剂的多个捕获剂的表面; 并通过提供使得能够与分析物结合的多价标签试剂与一种或多种捕获剂结合的条件来捕获分析物。

    Detection of antigens carried by erythrocytes and of anti-erythrocyte antibodies
    8.
    发明授权
    Detection of antigens carried by erythrocytes and of anti-erythrocyte antibodies 有权
    检测由红细胞和抗红细胞抗体携带的抗原

    公开(公告)号：US08580531B2

    公开(公告)日：2013-11-12

    申请号：US12601827

    申请日：2008-06-06

    Applicant: Frederic Buffiere ,  Yves Raisin ,  Eliane Rivalin ,  Amparo Sanjuan

    Inventor： Frederic Buffiere ,  Yves Raisin ,  Eliane Rivalin ,  Amparo Sanjuan

    IPC: G01N33/00 ,  G01N33/554

    CPC classification number: G01N33/80 ,  G01N33/537 ,  G01N33/54333 ,  Y10T436/101666 ,  Y10T436/25125

    Abstract: The invention relates to a method for detecting a plurality of antigenic molecules carried by erythrocytes and/or a plurality of anti-erythrocyte antibodies, said antigenic molecules carried by the erythrocytes consisting of antigenic molecules carried not only by the erythrocytes, but also by at least one other cell population, other than the blood group antigen molecules, said method comprising bringing a sample into contact with distinguishable beads, on which are attached a) antibodies specific for said antigens, or b) erythrocytes or erythrocyte membrane fragment.

    Abstract translation: 本发明涉及一种用于检测由红细胞和/或多种抗红细胞抗体携带的多种抗原性分子的方法，所述抗原分子由不仅由红细胞携带的抗原分子组成的红细胞携带，而且至少 所述方法包括使样品与可区分的珠接触，所述可区别的珠与所述抗原特异的a）抗体连接，或b）红细胞或红细胞膜片段。

    MOBILITY SHIFT ASSAYS FOR DETECTING ANTI-TNF ALPHA DRUGS AND AUTOANTIBODIES
    10.
    发明申请
    MOBILITY SHIFT ASSAYS FOR DETECTING ANTI-TNF ALPHA DRUGS AND AUTOANTIBODIES 审中-公开
    用于检测抗TNF ALPHA药物和自动物质的移动性移动测定

    公开(公告)号：US20130295685A1

    公开(公告)日：2013-11-07

    申请号：US13859664

    申请日：2013-04-09

    Applicant: NESTEC S.A.

    Inventor： Sharat Singh ,  Scott Hauenstein ,  Linda Ohrmund

    IPC: G01N33/537

    CPC classification number: G01N33/537 ,  G01N33/564 ,  G01N33/6863 ,  G01N33/9493 ,  G01N2333/7151 ,  G01N2800/52

    Abstract: The present invention provides assays for detecting and measuring the presence or level of anti-TNFα drugs and/or the autoantibodies to anti-TNFα drugs in a sample. The present invention is useful for optimizing therapy and monitoring patients receiving anti-TNFα drug therapeutics to detect the presence or level of autoantibodies against the drug. The present invention also provides methods for selecting therapy, optimizing therapy, and/or reducing toxicity in subjects receiving anti-TNFα drugs for the treatment of TNFα-mediated disease or disorders.

    Abstract translation: 本发明提供用于检测和测量样品中抗TNFalpha药物的存在或水平和/或抗TNFalpha药物的自身抗体的测定法。 本发明可用于优化治疗和监测接受抗TNFalpha药物治疗剂的患者，以检测针对药物的自身抗体的存在或水平。 本发明还提供了用于选择接受抗TNFalpha药物治疗TNFα介导的疾病或病症的受试者接受治疗，优化治疗和/或降低毒性的方法。

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