公开(公告)号：US09084831B2

公开(公告)日：2015-07-21

申请号：US13050672

申请日：2011-03-17

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

IPC分类号： A61K51/08

CPC分类号： A61K51/088 ,  A61K51/08

摘要： A precursor of a molecular probe for imaging of pancreatic islets is provided. The precursor includes a polypeptide represented by any one of the following formulae (1) to (12), or a polypeptide having a homology with the foregoing polypeptide: *-DLSKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (1) *-LSKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (2) *-SKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (3) *-KQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (4) *-DLSK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (5) *-LSK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (6) *-SK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (7) *-K*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (8) DLSK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (9) LSK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (10) SK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (11) K*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (12)

摘要翻译： 提供了用于胰岛成像的分子探针的前体。 前体包括由下列通式（1）至（12）中的任一个表示的多肽或与前述多肽具有同源性的多肽：* -DLSKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（1）* -LSKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（ 2）* -SKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（3）* -KQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（4）* -DLSK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（5）* -LSK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（6）* -SK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 7）* -K * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（8）DLSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（9）LSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（10）SK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（11）K * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2 12）

公开(公告)号：US07780828B2

公开(公告)日：2010-08-24

申请号：US10585295

申请日：2005-01-07

申请人： Hideaki Yamaoka ,  Kenji Nagakawa ,  Mitsuhiro Hoshijima ,  Sadaaki Kimura

发明人： Hideaki Yamaoka ,  Kenji Nagakawa ,  Mitsuhiro Hoshijima ,  Sadaaki Kimura

IPC分类号： G01N27/327

CPC分类号： C12Q1/006

摘要： The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).

摘要翻译： 本发明涉及一种分析仪器（1），其包括用于移动含有血细胞的样品的流路（5），用于将样品引入流路（5）的引入口（50），试剂部分 ）和电子检测介质（52），用于获得与所传送的电子数量相关的用于分析样品中包含的分析对象成分所需的信息。 试剂部（51）包含用于将从样品中的分析对象成分获取的电子供给到电子检测介质（52）的电子介体，并且所述试剂部的至少一部分位于与所述引入口（50）相邻的位置， 。

公开(公告)号：US08784624B2

公开(公告)日：2014-07-22

申请号：US12309222

申请日：2007-07-11

申请人： Koji Katsuki ,  Konomu Hirao ,  Kenji Nagakawa ,  Masashi Okamoto ,  Yoshiaki Fujinawa

发明人： Koji Katsuki ,  Konomu Hirao ,  Kenji Nagakawa ,  Masashi Okamoto ,  Yoshiaki Fujinawa

IPC分类号： G01N27/327

CPC分类号： C12Q1/001

摘要： The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

摘要翻译： 本发明涉及一种酶电极，包括：碳粒子; 保持在碳粒子上的金属粒子，具有对氧化还原反应的催化活性的金属粒子; 一种氧化还原酶。 本发明的酶电极还包括提高电阻的高电阻粒子，高电阻粒子化学稳定。 高电阻粒子例如含有无机物质。 无机物质例如是氧化铝或绿土。

公开(公告)号：US20110171129A1

公开(公告)日：2011-07-14

申请号：US13050672

申请日：2011-03-17

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

IPC分类号： A61K51/08 ,  C07K14/00 ,  C07K1/13

CPC分类号： A61K51/088 ,  A61K51/08

摘要： A precursor of a molecular probe for imaging of pancreatic islets is provided. The precursor includes a polypeptide represented by any one of the following formulae (1) to (12), or a polypeptide having a homology with the foregoing polypeptide: *-DLSKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (1) *-LSKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (2) *-SKQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (3) *-KQMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (4) *-DLSK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (5) *-LSK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (6) *-SK*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (7) *-K*QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (8) DLSK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (9) LSK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (10) SK*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (11) K*QMEEEAVRLFIEWLK*NGGPSSGAPPPS-NH2 (12)

摘要翻译： 提供了用于胰岛成像的分子探针的前体。 前体包括由下列通式（1）至（12）中的任一个表示的多肽或与前述多肽具有同源性的多肽：* -DLSKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（1）* -LSKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（ 2）* -SKQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（3）* -KQMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（4）* -DLSK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（5）* -LSK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（6）* -SK * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 7）* -K * QMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（8）DLSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（9）LSK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（10）SK * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2（11）K * QMEEEAVRLFIEWLK * NGGPSSGAPPPS-NH2 12）

公开(公告)号：US20100187107A1

公开(公告)日：2010-07-29

申请号：US12309222

申请日：2007-07-11

申请人： Koji Katsuki ,  Konomu Hirao ,  Kenji Nagakawa ,  Masashi Okamoto ,  Yoshiaki Fujinawa

发明人： Koji Katsuki ,  Konomu Hirao ,  Kenji Nagakawa ,  Masashi Okamoto ,  Yoshiaki Fujinawa

IPC分类号： G01N27/26

CPC分类号： C12Q1/001

摘要： The present invention relates to an enzyme electrode including: a carbon particle; a metal particle held on the carbon particle, the metal particle having a catalytic activity against a redox reaction; a redox enzyme. The enzyme electrode of the present invention further includes a high-resistance particle enhancing an electrical resistance, the high-resistance particle being chemically stable. The high-resistance particle contains an inorganic substance, for example. The inorganic substance is aluminum oxide or smectite, for example.

摘要翻译： 本发明涉及一种酶电极，包括：碳粒子; 保持在碳粒子上的金属粒子，具有对氧化还原反应的催化活性的金属粒子; 一种氧化还原酶。 本发明的酶电极还包括提高电阻的高电阻粒子，高电阻粒子化学稳定。 高电阻粒子例如含有无机物质。 无机物质例如是氧化铝或绿土。

公开(公告)号：US08574896B2

公开(公告)日：2013-11-05

申请号：US12469403

申请日：2009-05-20

申请人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

发明人： Kenji Nagakawa ,  Tomomichi Tsujimoto ,  Susumu Nishino ,  Masaaki Teramoto ,  Yoshiyuki Kawase

IPC分类号： C12M1/34

CPC分类号： G01N21/78 ,  C12Q1/26 ,  C12Q1/32

摘要： The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

摘要翻译： 本发明提供一种可以在短时间内进行简单可靠的分析的比色法。 该方法包括将电子从分析物转移到通过使用氧化还原酶通过介质还原产生颜色的着色剂; 并通过测量着色剂中产生的颜色来对分析物进行定性或定量分析。 该方法的酶反应是单阶段反应，并且通过介体发生颜色产生反应。 因此，可以在短时间内进行测量。 由于该反应既不需要过氧化氢也不需要氧气，因此测量值非常可靠。

公开(公告)号：US20120107239A1

公开(公告)日：2012-05-03

申请号：US13148303

申请日：2010-03-16

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa

IPC分类号： A61K51/04 ,  C07D209/44

CPC分类号： A61K51/0446

摘要： A precursor of a molecular probe for imaging of pancreatic islets is a compound expressed as the following formula (I): wherein -V-X represents a substituent on a benzene ring, V represents a bond, R1, of OR1, R1 represents a C1-C6 alkylene group, R2 represents H (hydrogen atom), a C1-C6 alkyl group, a C7-C10 aralkyl group, or a protecting group, X represents a OMs group, a OTs group, a OTf group, Br (bromine atom), or I (iodine atom), and carbon marked with * is an asymmetric carbon atom.

摘要翻译： 用于胰岛成像的分子探针的前体是以下式（I）表示的化合物：其中-VX表示苯环上的取代基，V表示OR1的键，R1表示C1-C6 亚烷基，R2表示H（氢原子），C1-C6烷基，C7-C10芳烷基或保护基，X表示OMs基，OTs基，OTf基，Br（溴原子） 或I（碘原子），标有*的碳是不对称碳原子。

公开(公告)号：US20090017483A1

公开(公告)日：2009-01-15

申请号：US10585295

申请日：2005-01-07

申请人： Hideaki Yamaoka ,  Kenji Nagakawa ,  Mitsuhiro Hoshijima ,  Sadaaki Kimura

发明人： Hideaki Yamaoka ,  Kenji Nagakawa ,  Mitsuhiro Hoshijima ,  Sadaaki Kimura

IPC分类号： C12Q1/54 ,  C12M1/34 ,  C12M1/36 ,  C12Q1/02

CPC分类号： C12Q1/006

摘要： The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).

摘要翻译： 本发明涉及一种分析仪器（1），其包括用于移动含有血细胞的样品的流路（5），用于将样品引入流路（5）的引入口（50），试剂部分 ）和电子检测介质（52），用于获得与所传送的电子数量相关的用于分析样品中包含的分析对象成分所需的信息。 试剂部（51）包含用于将从样品中的分析对象成分获取的电子供给到电子检测介质（52）的电子介体，并且所述试剂部的至少一部分位于与所述引入口（50）相邻的位置， 。

公开(公告)号：US08642008B2

公开(公告)日：2014-02-04

申请号：US12872837

申请日：2010-08-31

申请人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Nobuya Inagaki ,  Hideo Saji ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Yu Ogawa ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： A61K51/08

CPC分类号： A61K51/088

摘要： To provide a molecular probe for imaging of pancreatic islets. A molecular probe for use in imaging of pancreatic islets is provided. The molecular probe includes any one of the following polypeptides: polypeptides represented by the following formulae (1), (5), and (9); and polypeptides having homology with the foregoing polypeptides: Z-DLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (1) Z-DLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2 (5) B-DLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2 (9) where X in the formulae (1) and (5) and B- in the formula (9) indicate that an amino group is labeled with a group represented by the formula (I) below having an aromatic ring, wherein A represents either an aromatic hydrocarbon group or an aromatic heterocyclic group, R1 represents a substituent that contains radioactive iodine, R2 represents either a hydrogen atom or a substituent different from that represented by R1, and R3 represents any one of a bond, a methylene group, and an oxymethylene group.

摘要翻译： 为胰岛成像提供分子探针。 提供了一种用于胰岛成像的分子探针。 分子探针包括以下多肽之一：由下式（1），（5）和（9）表示的多肽。 和与前述多肽具有同源性的多肽：Z-DLSXQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（1）Z-DLSKQMEEEAVRLFIEWLXNGGPSSGAPPPS-NH2（5）B-DLSKQMEEEAVRLFIEWLKNGGPSSGAPPPS-NH2（9）其中式（1）和（5）中的X和 式（9）表示氨基用具有芳环的下式（I）表示的基团标记，其中A表示芳香族烃基或芳香族杂环基，R 1表示含有放射性碘的取代基， R2表示氢原子或与R1表示的不同的取代基，R3表示键，亚甲基和氧亚甲基中的任一个。

公开(公告)号：US20110081663A1

公开(公告)日：2011-04-07

申请号：US12893896

申请日：2010-09-29

申请人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

发明人： Hideo Saji ,  Nobuya Inagaki ,  Kentaro Toyoda ,  Hiroyuki Kimura ,  Konomu Hirao ,  Kenji Nagakawa ,  Hirokazu Matsuda

IPC分类号： G01N33/53 ,  C07K14/00

CPC分类号： A61K51/08

摘要： A molecular probe for imaging of pancreatic islets is provided. The molecular probe includes a polypeptide represented by the following formula (1), or a polypeptide that has a homology with the foregoing polypeptide. (SEQ ID NO. 1) Z-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSX-NH2 (1) Wherein “X” represents a lysine residue, an amino group of a side chain of the lysine residue being labeled with a group represented by the following chemical formula (I), wherein A represents an aromatic hydrocarbon group or an aromatic heterocyclic group; R1 represents a substituent that contains 11C, 13N, 15O, 18F, 64Cu, 67Ga, 68Ga, 75Br, 76Br, 77Br, 99mTc, 111In, 123I, 124I, 125I, or 131I; R2 represents either a hydrogen atom, or a substituent different from that represented by R1; and R3 represents any one of a bond, an alkylene group having 1 to 6 carbon atoms, and an oxyalkylene group having 1 to 6 carbon atoms.

摘要翻译： 提供了用于胰岛成像的分子探针。 分子探针包括由下式（1）表示的多肽，或与前述多肽具有同源性的多肽。 （SEQ ID NO.1）Z-HGEGTFTSDLSKQMEEEAVRLFIEWLKNGGPSSGAPPPSX-NH2（1）其中，“X”表示赖氨酸残基，赖氨酸残基的侧链的氨基用下述化学式（I）表示的基团标记， 其中A表示芳族烃基或芳族杂环基; R1表示含有11C，13N，150C，18F，64Cu，67Ga，68Ga，75Br，76Br，77Br，99mTc，111In，123I，124I，125I或131I的取代基; R2表示氢原子或与R1表示的取代基不同的取代基; R3表示碳原子数1〜6的亚烷基，碳原子数1〜6的氧亚烷基。