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10 条记录 (耗时 0.016 秒)

    Protease and process for production and use thereof
    2.
    发明授权
    Protease and process for production and use thereof 失效
    蛋白酶及其生产和使用的方法

    公开(公告)号：US4650763A

    公开(公告)日：1987-03-17

    申请号：US722356

    申请日：1985-04-12

    申请人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Takaharu Tanaka

    发明人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Takaharu Tanaka

    IPC分类号： C12N9/60 ,  C12P21/06 ,  C12R1/85 ,  C12R1/865 ,  C12N9/50

    CPC分类号： C12P21/06 ,  C12N9/60 ,  Y10S435/942

    摘要： Disclosed is a new protease having the following properties: (1) it is able to hydrolitically cleave a peptide bond between two adjacent basic amino acids in a peptide chain; (2) it has a molecular weight of about 43,000 as determined by electrophoresis; (3) it is inhibited by phenylmethylsulphonyl fluoride and diisopropyl fluorophosphate, but is not inhibited by monoiodoacetate, p-chloromercuribenzoic acid, ethylenediaminetetraacetic acid, 1,10-phenanthroline, tosyl-L-lysine chloromethyl ketone, and leupeptin. The protease can be produced by culturing Saccharomyces cerevisiae, and recovering purification by conventional methods, and is useful as a processing enzyme for conversion of a prohormone to an active hormone.

    摘要翻译： 公开了具有以下特性的新蛋白酶：（1）能够水解切割肽链中两个相邻碱性氨基酸之间的肽键; （2）通过电泳测定其分子量约为43,000; （3）被苯基甲基磺酰氟和氟代磷酸二异丙酯抑制，但不受单碘乙酸，对氯乙基邻苯二甲酸，乙二胺四乙酸，1,10-菲咯啉，甲苯磺酰-L-赖氨酸氯甲基酮和亮抑酶肽抑制。 可以通过培养酿酒酵母并通过常规方法回收纯化来生产蛋白酶，并且可用作将激素原转化为活性激素的加工酶。

    C-terminal .alpha.-amidating enzyme and process for production thereof
    3.
    发明授权
    C-terminal .alpha.-amidating enzyme and process for production thereof 失效
    C-末端α-酰胺化酶及其制备方法

    公开(公告)号：US5360727A

    公开(公告)日：1994-11-01

    申请号：US921600

    申请日：1992-08-03

    申请人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Masayasu Kojima

    发明人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Masayasu Kojima

    IPC分类号： C12N9/80 ,  C12N9/02 ,  C12N9/00 ,  C12P21/00

    CPC分类号： C12N9/0071 ,  C12Y114/17003

    摘要： The invention relates to a C-terminal .alpha.-amidating enzyme of porcine origin having the following properties: (1) the action is on a peptide or protein represented by the formula:X-R-Gly,wherein Gly represents a C-terminal glycine residue, R represents an amino acid residue to be .alpha.-amidated, and X represents a remaining portion of the peptide or protein to convert it to a peptide or protein represented by the formula:X-R-NH.sub.2,wherein R-NH.sub.2 represents a C-terminal .alpha.-amidated amino acid residue and X represents a remaining portion of the peptide or protein; (2) the optimal pH is 6.5 to 8.5; (3) the molecular weight is about 92,000 as determined by SDS-polyacrylamide gel electrophoresis; and (4) it contains the following peptide fragment:. . . Glu-Ala-Pro-Leu-Leu-Ile-Leu-Gly . . . .Further, the invention relates to a process for the production of the C-terminal .alpha.-amidating enzyme comprising the steps of extracting and purifying the enzyme from porcine atrium cordis exhibiting the enzyme activity.

    摘要翻译： 本发明涉及具有以下性质的猪来源的C-末端α-酰胺化酶：（1）作用是由下式表示的肽或蛋白：XR-Gly，其中Gly表示C-末端甘氨酸残基， R表示待酰胺化的氨基酸残基，X表示肽或蛋白质的剩余部分，以将其转化为由式XR-NH 2表示的肽或蛋白质，其中R-NH 2表示C末端α 酰胺化氨基酸残基，X表示肽或蛋白质的剩余部分; （2）最适pH为6.5〜8.5; （3）通过SDS-聚丙烯酰胺凝胶电泳测定分子量约为92,000; 和（4）它含有以下肽片段：。 。 。 Glu-Ala-Pro-Leu-Leu-Ile-Leu-Gly。 。 。 。 此外，本发明涉及一种生产C-末端α-酰胺化酶的方法，其包括从显示酶活性的猪心室提取和纯化酶的步骤。

    C-terminal alpha-amidating enzyme and process for production and use thereof
    4.
    发明授权
    C-terminal alpha-amidating enzyme and process for production and use thereof 失效
    C-末端α-酰胺化酶及其生产和使用的方法

    公开(公告)号：US4921797A

    公开(公告)日：1990-05-01

    申请号：US58919

    申请日：1987-06-05

    申请人： Hisayuki Matsuo ,  Kensaku Mizuno

    发明人： Hisayuki Matsuo ,  Kensaku Mizuno

    IPC分类号： C12N9/80 ,  C12N9/02

    CPC分类号： C12N9/0071 ,  C12Y114/17003

    摘要： C-terminal .alpha.-amidating enzyme preparations, including preparations AE-I, AE-II, AE-IIa and AE-IIb, from the skin of Xenopus laevis, wherein all components can convert a peptide having a glycine residue at its C-terminal to a C-terminal amidated peptide lacking the glycine residue, and have a common N-terminal amino acid sequence represented by Ser-Leu-Ser---, and AE-I and AE-IIa have a molecular weight of about 39,000, AE-IIb has a molecular weight of about 34,000, and AE-II comprises two components having molecular weight of about 39,000 and 34,000; a process for production of the above-mentioned enzyme preparations; and a process for .alpha.-amidation of a peptide by using the above mentioned enzyme preparations.

    摘要翻译： C-末端α-酰胺化酶制剂，包括来自非洲爪蟾皮肤的制剂AE-I，AE-II，AE-IIa和AE-IIb，其中所有组分可以在其C-末端转化具有甘氨酸残基的肽 具有缺少甘氨酸残基的C末端酰胺化肽，并且具有由Ser-Leu-Ser ---表示的常见N-末端氨基酸序列，AE-I和AE-IIa具有约39,000的分子量，AE -IIb具有约34,000的分子量，并且AE-II包含分子量为约39,000和34,000的两种组分; 制备上述酶制剂的方法; 以及通过使用上述酶制剂对肽进行α-酰胺化的方法。

    DNA encoding a KEX2 endoprotease without a C-terminal hydrophobic region
    6.
    发明授权
    DNA encoding a KEX2 endoprotease without a C-terminal hydrophobic region 失效
    编码没有C末端疏水区的KEX2内切蛋白的DNA

    公开(公告)号：US5234830A

    公开(公告)日：1993-08-10

    申请号：US916627

    申请日：1992-07-22

    申请人： Takehiro Oshima ,  Kensaku Mizuno

    发明人： Takehiro Oshima ,  Kensaku Mizuno

    IPC分类号： C12N9/60

    CPC分类号： C12N9/60 ,  C07K2319/00

    摘要： A KEX2 endoprotease produced by a recombinant DNA technique, a KEX2 endoprotease shortened at the C-terminal of a native enzyme and still containing a C-terminal hydrophobic region, a soluble KEX2 endoprotease without a C-terminal hydrophobic region; DNA's coding for the above-mentioned enzymes; expression plasmids containing the DNA; hosts transformed with the plasmid; a process for production of the above-mentioned enzymes using the transformed host; and a process for production of biologically active polypeptide using the above-mentioned enzyme.

    摘要翻译： 通过重组DNA技术产生的KEX2内切蛋白酶，在天然酶的C末端缩短且仍含有C末端疏水区域的KEX2内切蛋白酶，不具有C末端疏水区域的可溶性KEX2内切蛋白酶; DNA编码上述酶; 含有DNA的表达质粒; 用质粒转化的宿主; 使用转化的宿主生产上述酶的方法; 以及使用上述酶生产生物活性多肽的方法。

    Protease and process for production and use thereof
    7.
    发明授权
    Protease and process for production and use thereof 失效
    蛋白酶及其生产和使用的方法

    公开(公告)号：US4704359A

    公开(公告)日：1987-11-03

    申请号：US917210

    申请日：1986-10-09

    申请人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Takaharu Tanaka

    发明人： Hisayuki Matsuo ,  Kensaku Mizuno ,  Takaharu Tanaka

    IPC分类号： C12N9/60 ,  C12P21/06 ,  C12R1/85 ,  C12R1/865

    CPC分类号： C12P21/06 ,  C12N9/60 ,  Y10S435/942

    摘要： Disclosed is a new protease having the following properties: (1) it is able to hydrolitically cleave a peptide bond between two adjacent basic amino acids in a peptide chain; (2) it has a molecular weight of about 43,000 as determined by electrophoresis; (3) it is inhibited by phenylmethylsulphonyl fluoride and diisopropyl fluorophosphate, but is not inhibited by monoiodoacetate, p-chloromercuribenzoic acid, ethylenediaminetetraacetic acid, 1,10-phenanthroline, tosyl-L-lysine chloromethyl ketone, and leupeptin. The protese can be produced by culturing Saccharomyces cerevisiae, and recovering purification by conventional methods, and is useful as a processing enzyme for conversion of a prohormone to an active hormone.