公开(公告)号：US07354615B2

公开(公告)日：2008-04-08

申请号：US10481893

申请日：2002-07-19

申请人： Toyokazu Kaneko ,  Yasuo Yonou ,  Naoto Hirano ,  Shigemitsu Abe ,  Kunihiko Toumori

发明人： Toyokazu Kaneko ,  Yasuo Yonou ,  Naoto Hirano ,  Shigemitsu Abe ,  Kunihiko Toumori

IPC分类号： A23L1/221 ,  B01D9/00

CPC分类号： C07H1/00 ,  C07H19/20

摘要： Disclosed herein are a process for producing mixed crystals of disodium 5′-guanylate and disodium 5′-inosinate which comprises precipitating mixed crystals of disodium 5′-guanylate and disodium 5′-inosinate (I+G mixed crystals) by adding an aqueous mixed solution of disodium 5′-guanylate and disodium 5′-inosinate and a hydrophilic organic solvent at the same time into a crystallization vessel in such manner that the ratio of the hydrophilic organic solvent to the liquid phase in the crystallization vessel is maintained in a range of 30 to 70 vol %, as well as such process for producing I+G mixed crystals wherein said producing of I+G mixed crystals is carried out by seeding crystallization wherein crystals of 5′-IMP2Na or/and I+G mixed crystals are used as seed crystals. According to these production processes, the by-production of amorphous solids of 5′-GMP2Na which adversely affect separability of the crystals concerned, is inhibited, and therefore, such mixed crystals having a good separability without contamination of such amorphous solids, can be produced in a high productivity.

摘要翻译： 本文公开了一种生产5'-鸟苷酸二钠和5'-肌苷酸二钠的混合晶体的方法，该方法包括通过加入含水混合物来沉淀5'-鸟苷酸二钠和5'-肌苷酸二钠（I + G混合晶体）的混合物 5'-鸟苷酸二钠和5'-肌苷酸二钠和亲水性有机溶剂的溶液同时放入结晶容器中，使得结晶容器中的亲水性有机溶剂与液相的比率保持在一定范围内 为30〜70体积％，以及这种I + G混合晶体的制造方法，其中所述I + G混晶的制造是通过晶种结晶进行的，其中5'-IMP2Na或/和I + G混合晶体的晶体为 用作晶种。 根据这些制造方法，抑制了对相关晶体的分离性有不利影响的5-GMP2Na的无定形固体的副产物，因此可以制备这种具有良好分离性而不会污染这种无定形固体的混合晶体 在高生产力。

公开(公告)号：US07294491B2

公开(公告)日：2007-11-13

申请号：US11265288

申请日：2005-11-03

申请人： Hiroshi Ueda ,  Kunihiko Toumori

发明人： Hiroshi Ueda ,  Kunihiko Toumori

IPC分类号： C12P13/14 ,  C12N1/12 ,  C12N1/36 ,  C12N1/20

CPC分类号： C12P13/14

摘要： The present invention provides a method for producing L-glutamic acid by fermentation, by culturing in a liquid medium a microorganism that can metabolize a carbon source at a specific pH, and wherein said medium contains a carbon source and L-glutamic acid at a saturation concentration, and wherein said microorganism is able to cause accumulation of an amount of L-glutamic acid in a liquid medium having said pH, wherein said amount exceeds the amount of L-glutamic acid at said saturation concentration when the pH of the medium is controlled so that L-glutamic acid is precipitated, making L-lysine exist in the medium when L-glutaminc acid concentration is lower than the concentration at which natural crystallization of L-glutamic acid occurs, and precipitating the α-form crystals of L-glutamic acid.

摘要翻译： 本发明提供了通过发酵生产L-谷氨酸的方法，通过在液体培养基中培养能够在特定pH下代谢碳源的微生物，并且其中所述培养基含有饱和的碳源和L-谷氨酸 浓度，并且其中所述微生物能够在具有所述pH的液体培养基中引起一定量的L-谷氨酸的累积，其中当所述培养基的pH被控制时，所述量超过所述饱和浓度时的L-谷氨酸的量 使得L-谷氨酸浓度低于L-谷氨酸的天然结晶浓度时，L-赖氨酸存在于培养基中，使L-谷氨酸沉淀，从而使L-谷氨酸的α型晶体 酸。

公开(公告)号：US20060084151A1

公开(公告)日：2006-04-20

申请号：US11265288

申请日：2005-11-03

申请人： Hiroshi Ueda ,  Kunihiko Toumori

发明人： Hiroshi Ueda ,  Kunihiko Toumori

IPC分类号： C12P13/14 ,  C12N1/21

CPC分类号： C12P13/14

摘要： The present invention provides a method for producing L-glutamic acid by fermentation, by culturing in a liquid medium a microorganism that can metabolize a carbon source at a specific pH, and wherein said medium contains a carbon source and L-glutamic acid at a saturation concentration, and wherein said microorganism is able to cause accumulation of an amount of L-glutamic acid in a liquid medium having said pH, wherein said amount exceeds the amount of L-glutamic acid at said saturation concentration when the pH of the medium is controlled so that L-glutamic acid is precipitated, making L-lysine exist in the medium when L-glutaminc acid concentration is lower than the concentration at which natural crystallization of L-glutamic acid occurs, and precipitating the α-form crystals of L-glutamic acid.

摘要翻译： 本发明提供了通过发酵生产L-谷氨酸的方法，通过在液体培养基中培养能够在特定pH下代谢碳源的微生物，并且其中所述培养基含有饱和的碳源和L-谷氨酸 浓度，并且其中所述微生物能够在具有所述pH的液体培养基中引起一定量的L-谷氨酸的累积，其中当所述培养基的pH被控制时，所述量超过所述饱和浓度时的L-谷氨酸的量 使得L-谷氨酸浓度低于L-谷氨酸的天然结晶浓度时，L-赖氨酸存在于培养基中，使L-谷氨酸沉淀，从而使L-谷氨酸的α型晶体 酸。