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公开(公告)号：US11046964B2
公开(公告)日：2021-06-29
申请号：US16849532
申请日：2020-04-15
申请人： Pfenex Inc.
发明人： Russell J. Coleman , Torben Bruck
摘要： Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.
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2.发明授权Process for purifying recombinant Plasmodium falciparum circumsporozoite protein 有权重组恶性疟原虫环子孢子蛋白纯化方法公开(公告)号：US09169304B2
公开(公告)日：2015-10-27
申请号：US13844261
申请日：2013-03-15
申请人： Pfenex Inc.
发明人： Jeffrey Allen , Greg Cantin , Ryan Haverstock
IPC分类号： A23J1/10 , C07K1/10 , C07K14/445 , A61K39/00
CPC分类号： A61K47/02 , A61K38/17 , A61K2039/55566 , C07K14/445 , Y02A50/411
摘要： The present invention relates to processes for purifying high-quality recombinant Plasmodium falciparum circumsporozoite protein at high yields.
摘要翻译： 本发明涉及以高产率纯化高质量重组恶性疟原虫环子孢子蛋白的方法。
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公开(公告)号：US20190127744A1
公开(公告)日：2019-05-02
申请号：US16163421
申请日：2018-10-17
申请人： Pfenex Inc.
发明人： Russell J. Coleman
摘要： Provided herein are bacterial leader sequences for periplasmic expression of heterologous proteins, fusion proteins comprising bacterial leader sequences, and methods of expression of same.
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4.发明申请METHOD FOR PRODUCING SOLUBLE RECOMBINANT INTERFERON PROTEIN WITHOUT DENATURING 有权生产可溶性重组干扰素蛋白的方法，无需去除公开(公告)号：US20160032345A1
公开(公告)日：2016-02-04
申请号：US14884081
申请日：2015-10-15
申请人： Pfenex Inc.
发明人： Jeffrey Allen , Ping-Hua Feng , Anant Patkar , Keith L. Haney , Lawrence Chew , Lei Lei Phokham Sengchanthalangsy
IPC分类号： C12P21/02 , C07K14/555
CPC分类号： C12P21/02 , A61K38/21 , C07K14/555
摘要： The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host.
摘要翻译： 本发明涉及细菌宿主中重组蛋白质生产领域。 它还涉及从不溶性级分中提取可溶性活性重组蛋白，而不需要使用变性，而不需要重折叠步骤。 特别地，本发明涉及从细菌宿主获得高水平的可溶性重组型1型干扰素蛋白的制备方法。
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公开(公告)号：US20150361405A1
公开(公告)日：2015-12-17
申请号：US14531833
申请日：2014-11-03
申请人： Pfenex Inc.
发明人： Diane M. Retallack , Lawrence Chew
IPC分类号： C12N9/10 , C12P21/02 , C07K14/21 , C07K14/28 , C07K14/235
CPC分类号： C12N9/1051 , C07K14/21 , C07K14/235 , C07K14/28 , C07K14/34 , C07K2319/036 , C12N9/1077 , C12N15/78 , C12P21/00 , C12P21/02 , C12Y204/01 , C12Y204/02036 , C12Y304/24068 , G01N33/573
摘要： The present invention relates to the field of recombinant toxin protein production in bacterial hosts. In particular, the present invention relates to production processes for obtaining high levels of a recombinant CRM197, Diphtheria Toxin, Pertussis Toxin, Tetanus Toxoid Fragment C, Cholera Toxin B, Cholera holotoxin, and Pseudomonas Exotoxin A, from a bacterial host.
摘要翻译： 本发明涉及细菌宿主中重组毒素蛋白质生产领域。 特别地，本发明涉及从细菌宿主获得高水平的重组CRM197，白喉毒素，百日咳毒素，破伤风毒素片段C，霍乱毒素B，霍乱全毒素和假单胞菌外毒素A的生产方法。
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公开(公告)号：US20140162279A1
公开(公告)日：2014-06-12
申请号：US14071273
申请日：2013-11-04
申请人： PFENEX INC.
IPC分类号： C12N15/63
摘要： This invention is a process for improving the production levels of recombinant proteins or peptides or improving the level of active recombinant proteins or peptides expressed in host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recombinant protein expression. The process can improve protein production or can improve protein quality, for example, by increasing solubility of a recombinant protein.
摘要翻译： 本发明是改善重组蛋白或肽的生产水平或提高在宿主细胞中表达的活性重组蛋白或多肽的水平的方法。 本发明是比较表达重组蛋白的细胞的两种遗传图谱并修饰细胞以改变响应于重组蛋白表达上调的基因产物的表达的过程。 该方法可以改善蛋白质产生或者可以通过增加重组蛋白质的溶解度来改善蛋白质质量。
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公开(公告)号：US10981968B2
公开(公告)日：2021-04-20
申请号：US16135875
申请日：2018-09-19
申请人： Pfenex Inc.
发明人： Diane M. Retallack , Adam Chapman , Torben R. Bruck , Hongfan Jin
IPC分类号： C07K14/635 , C07K14/535 , C07K14/62 , C12N9/64
摘要： The present invention relates to the field of medicine, in particular, to the production of large amounts of a soluble recombinant polypeptide as part of a fusion protein comprising an N-terminal fusion partner linked to the polypeptide of interest.
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公开(公告)号：US20200248193A1
公开(公告)日：2020-08-06
申请号：US16849532
申请日：2020-04-15
申请人： Pfenex Inc.
发明人： Russell J. COLEMAN , Torben BRUCK
摘要： Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.
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公开(公告)号：US20190127743A1
公开(公告)日：2019-05-02
申请号：US16163398
申请日：2018-10-17
申请人： Pfenex Inc.
发明人： Russell J. COLEMAN , Torben BRUCK
CPC分类号： C12N15/78 , C12N9/82 , C12N15/67 , C12R1/39 , C12Y305/01001
摘要： Provided herein are methods of production of recombinant E. coli asparaginase. Methods herein allow production of asparaginase in Pseudomonadales host cells at high expression levels and having activity comparable to commercially available asparaginase preparations.
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公开(公告)号：US09611499B2
公开(公告)日：2017-04-04
申请号：US14884081
申请日：2015-10-15
申请人： Pfenex Inc.
发明人： Jeffrey Allen , Ping-Hua Feng , Anant Patkar , Keith L. Haney , Lawrence Chew , Lei Lei Phokham Sengchanthalangsy
IPC分类号： C07K14/555 , A61K38/21 , C12P21/02
CPC分类号： C12P21/02 , A61K38/21 , C07K14/555
摘要： The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host.
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