公开(公告)号：US06541273B1

公开(公告)日：2003-04-01

申请号：US09711210

申请日：2000-11-09

申请人： Robert S. Plaisance

发明人： Robert S. Plaisance

IPC分类号： G01N3014

CPC分类号： G01N30/482 ,  B01D15/265 ,  B01J20/103 ,  B01J20/20 ,  B01J20/28052 ,  B01J20/282 ,  B01J20/283 ,  B01J20/3204 ,  B01J20/3236 ,  B01J20/3248 ,  B01J20/3289 ,  B01J2220/52 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/62 ,  G01N30/461 ,  G01N30/6069 ,  G01N30/6091 ,  Y10T436/147777 ,  Y10T436/255

摘要： Methods and apparatus are disclosed which are useful for detecting the presence of various pesticides, including aryl pyrazoles such as fipronil. Pesticide residues are extracted from a variety of samples, including soil and/or water samples using techniques well known to those of skill in the art. The samples are then concentrated, and transferred to an solid phase extraction cartridge. The SPE cartridge includes activated charcoal, silica gel, magnesium-silica gel and an amino-functional silica sorbent, or suitable equivalents thereof. After cleanup, the eluate containing the analyte of interest can be concentrated to a standard sample size and the amount of pesticide residues quantitated. In a preferred embodiment, the column chromatography materials are assembled in a solid phase extraction cartridge with frits between the chromatography materials. This facilitates the use of the cartridge in standard automated analytical equipment, for example, robotic equipment.

摘要翻译： 公开了可用于检测各种农药（包括芳基吡唑如氟虫腈）的存在的方法和装置。 使用本领域技术人员熟知的技术从各种样品中提取农药残留物，包括土壤和/或水样品。 然后将样品浓缩，并转移至固相萃取柱。 SPE柱包括活性炭，硅胶，镁 - 硅胶和氨基官能二氧化硅吸附剂，或其合适的等同物。 清理后，可将含有目标分析物的洗脱液浓缩至标准样品量，并定量确定农药残留量。 在优选的实施方案中，将柱色谱材料组装在具有色谱材料之间的玻璃料的固相萃取柱中。 这有助于在标准自动分析设备（例如机器人设备）中使用墨盒。