公开(公告)号：US06541273B1

公开(公告)日：2003-04-01

申请号：US09711210

申请日：2000-11-09

申请人： Robert S. Plaisance

发明人： Robert S. Plaisance

IPC分类号： G01N3014

CPC分类号： G01N30/482 ,  B01D15/265 ,  B01J20/103 ,  B01J20/20 ,  B01J20/28052 ,  B01J20/282 ,  B01J20/283 ,  B01J20/3204 ,  B01J20/3236 ,  B01J20/3248 ,  B01J20/3289 ,  B01J2220/52 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/62 ,  G01N30/461 ,  G01N30/6069 ,  G01N30/6091 ,  Y10T436/147777 ,  Y10T436/255

摘要： Methods and apparatus are disclosed which are useful for detecting the presence of various pesticides, including aryl pyrazoles such as fipronil. Pesticide residues are extracted from a variety of samples, including soil and/or water samples using techniques well known to those of skill in the art. The samples are then concentrated, and transferred to an solid phase extraction cartridge. The SPE cartridge includes activated charcoal, silica gel, magnesium-silica gel and an amino-functional silica sorbent, or suitable equivalents thereof. After cleanup, the eluate containing the analyte of interest can be concentrated to a standard sample size and the amount of pesticide residues quantitated. In a preferred embodiment, the column chromatography materials are assembled in a solid phase extraction cartridge with frits between the chromatography materials. This facilitates the use of the cartridge in standard automated analytical equipment, for example, robotic equipment.

摘要翻译： 公开了可用于检测各种农药（包括芳基吡唑如氟虫腈）的存在的方法和装置。 使用本领域技术人员熟知的技术从各种样品中提取农药残留物，包括土壤和/或水样品。 然后将样品浓缩，并转移至固相萃取柱。 SPE柱包括活性炭，硅胶，镁 - 硅胶和氨基官能二氧化硅吸附剂，或其合适的等同物。 清理后，可将含有目标分析物的洗脱液浓缩至标准样品量，并定量确定农药残留量。 在优选的实施方案中，将柱色谱材料组装在具有色谱材料之间的玻璃料的固相萃取柱中。 这有助于在标准自动分析设备（例如机器人设备）中使用墨盒。

公开(公告)号：US06790669B1

公开(公告)日：2004-09-14

申请号：US10018629

申请日：2001-12-14

申请人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

发明人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

IPC分类号： G01N3014

CPC分类号： G01N30/88 ,  G01N30/78 ,  G01N2030/625 ,  G01N2030/8813 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U.S. Pat. No. 5,592,402, to identify at least one outlier peak. The sixth step is to determine the chemical identity of the outlier peak, for example, using gas chromatography/mass spectroscopy analysis of the outlier peak.

摘要翻译： 一种化学分析方法，用于确定受试生物材料如转基因植物材料和对照生物材料如基因未修饰植物材料之间的化学相关差异，该方法至少包括以下六个步骤。 第一步是用流体萃取剂如水，异丙醇和氢氧化钾的混合物接触主题生物材料，以产生受试者生物材料的流体提取物。 第二步是将控制生物材料与流体萃取剂接触以产生对照生物材料的流体萃取物。 第三步是对目标生物材料的流体提取物进行色谱分离，例如气相色谱或流体色谱法，以产生该物质生物材料的液体提取物的色谱图。 第四步是对对照生物材料的流体提取物进行色谱分析以产生对照生物材料的液体提取物的色谱图。 第五步是确定色谱图之间的差异，例如，使用美国专利No. 确定至少一个异常值峰值的第5,592,402号。 第六步是确定离子峰的化学特性，例如使用离子峰的气相色谱/质谱分析。