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    Methods for detecting molecules in a sample
    7.
    发明授权
    Methods for detecting molecules in a sample 有权

    公开(公告)号：US10578620B2

    公开(公告)日：2020-03-03

    申请号：US16227899

    申请日：2018-12-20

    申请人： The Francis Crick Institute Limited

    发明人： Banafshe Larijani ,  Peter Parker ,  Selvaraju Veeriah

    IPC分类号： G01N33/574 ,  G01N33/543

    摘要： The present invention relates to a method for detecting molecules. The method employs: at least two primary antibodies, wherein the first primary antibody binds to a first site on a molecule and the second primary antibody binds to a second site on a molecule, wherein the second site is different from the first site and wherein the first and second primary antibodies are immunologically distinct; at least two secondary antibodies, wherein the first secondary antibody is labelled with a fluorescence resonance energy transfer (FRET) donor and binds to the first primary antibody; and the second secondary antibody is conjugated or fused to an enzyme and binds the second primary antibody, wherein the first secondary antibody does not bind the second primary antibody and the second secondary antibody does not bind the first primary antibody; a conjugate comprising a FRET acceptor and a substrate specific for the enzyme, wherein when the substrate reacts with the enzyme, an activated conjugate forms, which activated conjugate binds to electron rich moieties on a molecular surface adjacent to the enzyme; wherein the method comprises: contacting a sample with the at least two primary antibodies; contacting the sample with the at least two secondary antibodies; performing a wash step; contacting the sample with the conjugate; and detecting any FRET signal generated by the FRET acceptor.

    ELECTROCHEMICAL PROBE
    8.
    发明申请
    ELECTROCHEMICAL PROBE 审中-公开

    公开(公告)号：US20180067075A1

    公开(公告)日：2018-03-08

    申请号：US15259435

    申请日：2016-09-08

    申请人： The Francis Crick Institute Limited

    发明人： Romeo-Robert RACZ ,  Mihaly KOLLO ,  Andreas SCHAEFER

    IPC分类号： G01N27/416 ,  H01R4/64 ,  C25D13/02 ,  C25D13/20 ,  C25D13/22 ,  G01N27/28

    摘要： An electrochemical probe comprises a wire bundle including two or more wire electrodes made of conducting material arranged alongside each other, and insulating material surrounding the electrodes. An impedance reducing layer of metal or metal oxide nano-structures is deposited on tips of the wire electrodes at a first end of the bundle. A functionalization layer is deposited on the impedance reducing layer at the first end of the bundle. Such a probe is particularly useful for electrochemical sensing applications such as neuronal scanning.

    THYMIC CONSTRUCTS AND USES THEREOF
    10.
    发明公开
    THYMIC CONSTRUCTS AND USES THEREOF 审中-公开

    公开(公告)号：US20240002791A1

    公开(公告)日：2024-01-04

    申请号：US18039849

    申请日：2021-12-08

    申请人： THE FRANCIS CRICK INSTITUTE LIMITED

    发明人： Paola BONFANTI

    IPC分类号： C12N5/078 ,  A61L27/38

    CPC分类号： C12N5/0634 ,  A61L27/3813 ,  A61L27/3895 ,  A61L2430/40

    摘要： The present invention provides a method of producing a thymic construct suitable for implantation into a subject, the method comprising the steps of: (i) providing an acellular scaffold; (ii) seeding the acellular scaffold with thymic epithelial cells having mesenchymal properties; and (iii) culturing the seeded scaffold to produce said construct. The thymic epithelial cells are preferably CD49f+ and may also be VIM+, TE-7+ and/or CD90+. The invention further provides pharmaceutical compositions, uses and therapies using thymic epithelial cells having mesenchymal properties.