公开(公告)号：US20180355310A1

公开(公告)日：2018-12-13

申请号：US15780236

申请日：2016-11-30

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jackie Y. YING ,  Nandanan ERATHODIYIL ,  Karthikeyan NARAYANAN ,  Andrew C.A. WAN

IPC: C12N5/00

CPC classification number: C12N5/0068 ,  B82Y5/00 ,  B82Y30/00 ,  C12N2533/30 ,  C12N2537/00 ,  C12N2539/00

Abstract: A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross-linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nanoparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol terminated nanoparticles. In addition, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine functionalized nanoparticles thereon.