公开(公告)号：US20180355310A1

公开(公告)日：2018-12-13

申请号：US15780236

申请日：2016-11-30

申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

发明人： Jackie Y. YING ,  Nandanan ERATHODIYIL ,  Karthikeyan NARAYANAN ,  Andrew C.A. WAN

IPC分类号： C12N5/00

CPC分类号： C12N5/0068 ,  B82Y5/00 ,  B82Y30/00 ,  C12N2533/30 ,  C12N2537/00 ,  C12N2539/00

摘要： A cell culture substrate comprising a substrate having a coating of a plurality of amine functionalized nanoparticles is disclosed. In one embodiment, the amine functionalized nanoparticle is a polymer of an acrylamide monomer, a cross-linker and an amine monomer. There is also provided a method of making the cell culture substrate either by drying the amine functionalized nanoparticles when spread onto the said substrate or by covalent linkages of the substrate with thiol terminated nanoparticles. In addition, there is provided a method of culturing stem cells on the cell culture substrate having a coating of a plurality of the amine functionalized nanoparticles thereon.

公开(公告)号：US20200283734A1

公开(公告)日：2020-09-10

申请号：US16628737

申请日：2018-09-07

申请人： AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

发明人： Karthikeyan NARAYANAN ,  Chan DU ,  Andrew Chwee Aun WAN

IPC分类号： C12N5/074 ,  A61K35/545

摘要： The present invention generally relates to methods of reprogramming cells using exosomes. The present invention also relates methods of screening drugs suitable for cancer treatment using the reprogrammed cells, obtained from the methods described herein. The present invention also relates to therapeutic uses of the reprogrammed cells, obtained from the methods described herein.