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    Reversible nuclear genetic system for male sterility in transgenic plants
    1.
    发明授权
    Reversible nuclear genetic system for male sterility in transgenic plants 有权
    在转基因植物中雄性不育的可逆核遗传系统

    公开(公告)号：US06399856B1

    公开(公告)日：2002-06-04

    申请号：US09195716

    申请日：1998-11-19

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： A01H100

    CPC分类号： C12N9/1007 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N15/8289 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

    摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物可逆地雄性不育。 特别地，本发明涉及显性阴性基因和花药特异性启动子的用途。 通过掺入抑制显性阴性基因的第二遗传构建体的植物中，雄性不育是逆转的。 本发明还涉及在植物中表现出作为花药特异性启动子的能力的新型DNA序列。

    Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility
    3.
    发明授权
    Transgenic plants and DNA comprising anther specific promoter 5126 and gene to achieve male sterility 失效
    转基因植物和包含花药特异性启动子5126和基因的DNA以实现雄性不育

    公开(公告)号：US5689051A

    公开(公告)日：1997-11-18

    申请号：US474556

    申请日：1995-06-07

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C12N15/00 ,  A01H1/06 ,  A01H4/00 ,  C12N15/05

    CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant male-sterile. In particular, the present invention relates to an isolated DNA molecule comprising an anther-specific promoter, designated 5126, its operable linkage to a DNA sequence encoding a gene product and transgenic plants comprising said DNA. More particularly the gene product linked to promoter 5126 disrupts the function or formation of pollen.

    摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物雄性不育。 特别地，本发明涉及分离的DNA分子，其包含命名为5126的花药特异性启动子，其与编码基因产物的DNA序列的可操作连接以及包含所述DNA的转基因植物。 更特别地，与启动子5126连接的基因产物破坏了花粉的功能或形成。

    Reversible nuclear genetic system for male sterility in transgenic plants
    7.
    发明授权
    Reversible nuclear genetic system for male sterility in transgenic plants 失效
    在转基因植物中雄性不育的可逆核遗传系统

    公开(公告)号：US5750868A

    公开(公告)日：1998-05-12

    申请号：US351899

    申请日：1994-12-08

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： A01H1/02 ,  C07K14/415 ,  C12N9/10 ,  C12N15/82 ,  C12H15/00 ,  A01H1/06 ,  A01H4/00 ,  C12H15/05

    CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N15/8216 ,  C12N15/8231 ,  C12N9/1007 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and structural genes capable of acting in a cascading fashion to produce a reversible effect on a plant phenotype. A first genetic construct comprising (i) an operator that is capable of controlling expression of a dominant negative gene, (ii) a dominant negative gene that, when expressed in a plant disrupts pollen formation or function, (iii) a tissue specific promoter that regulates the expression of a gene encoding a DNA-binding protein which binds to the operator and activates transcription, and (iv) a gene encoding a DNA binding region and an activating domain, causes male sterility in a plant. In particular, the present invention relates to the use of a DAM-methylase gene as a dominant negative gene and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene.

    摘要翻译： 可以通过用包括调节元件和能够以级联方式起作用的结构基因的遗传构建体转化植物以对植物表型产生可逆效应来改变植物发育。 第一遗传构建体，其包含（i）能够控制显性阴性基因表达的操纵子，（ii）当在植物中表达时破坏花粉形成或功能的显性负基因，（iii）组织特异性启动子， 调节编码与操作者结合并激活转录的DNA结合蛋白的基因的表达，和（iv）编码DNA结合区和激活结构域的基因在植物中引起雄性不育。 特别地，本发明涉及DAM-甲基化酶基因作为显性阴性基因和花药特异性启动子的用途。 通过掺入抑制显性阴性基因的第二遗传构建体的植物中，雄性不育是逆转的。

    Reversible nuclear genetic system for male sterility in transgenic plants
    9.
    发明授权
    Reversible nuclear genetic system for male sterility in transgenic plants 失效
    在转基因植物中雄性不育的可逆核遗传系统

    公开(公告)号：US06248935B1

    公开(公告)日：2001-06-19

    申请号：US08975674

    申请日：1997-11-28

    申请人： Andrew M. Cigan ,  Marc C. Albertsen

    发明人： Andrew M. Cigan ,  Marc C. Albertsen

    IPC分类号： C12N100

    CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/8216 ,  C12N15/8231 ,  Y10S47/01

    摘要： Plant development can be altered by transforming a plant with a genetic construct that includes regulatory elements and DNA sequences capable of acting in a fashion to inhibit pollen formation or function, thus rendering the transformed plant reversibly male-sterile. In particular, the present invention relates to the use of dominant negative genes and an anther-specific promoter. Male sterility is reversed by incorporation into a plant of a second genetic construct which represses the dominant negative gene. The invention also relates to novel DNA sequences which exhibit the ability to serve as anther-specific promoters in plants.

    摘要翻译： 可以通过用包括调节元件和能够以抑制花粉形成或功能的方式起作用的DNA序列的遗传构建体转化植物来改变植物发育，从而使转化的植物可逆地雄性不育。 特别地，本发明涉及显性阴性基因和花药特异性启动子的用途。 通过掺入抑制显性阴性基因的第二遗传构建体的植物中，雄性不育是逆转的。 本发明还涉及在植物中表现出作为花药特异性启动子的能力的新型DNA序列。