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    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING FEED RATE SHIFT
    2.
    发明公开
    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING FEED RATE SHIFT 审中-公开

    公开(公告)号：US20230287379A1

    公开(公告)日：2023-09-14

    申请号：US18018094

    申请日：2021-07-27

    Applicant: BASF SE

    Inventor： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Michael Morweiser ,  Georg Benjamin Wandrey

    IPC: C12N9/26 ,  C12N9/54

    CPC classification number: C12N9/2414 ,  C12N9/54 ,  C12Y304/21062

    Abstract: The present invention relates to the field of industrial fermentation. In particular, it relates to method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source at increasing rates, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source at a constant rate, at decreasing rates or at rates increasing less than the rates in step (b), wherein said constant rate or the starting rate of said decreasing rates or the starting rate of said rates increasing less than the rates in step (b) is below the maximum rate of the first cultivation phase. Further contemplated is a Bacillus host cell culture obtainable by said method.

    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT
    5.
    发明公开
    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING TEMPERATURE SHIFT 审中-公开

    公开(公告)号：US20230295681A1

    公开(公告)日：2023-09-21

    申请号：US18018083

    申请日：2021-07-27

    Applicant: BASF SE

    Inventor： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Michael Morweiser ,  Georg Benjamin Wandrey

    IPC: C12P21/02 ,  C12N9/26 ,  C12N9/24

    CPC classification number: C12P21/02 ,  C12N9/2411 ,  C12N9/2488

    Abstract: The present invention relates to the field of industrial fermentation. In particular, it relates to a method for cultivating a Bacillus host cell comprising the steps of (a) inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, (b) cultivating for a first cultivation phase the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation of the Bacillus host cell comprises the addition of at least one feed solution and wherein the cultivation during the first cultivation phase is carried out at a first temperature, and (c) cultivating for a second cultivation phase the Bacillus host cell culture obtained in step (b) under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest, wherein the cultivation comprises the addition of at least one feed solution and wherein the cultivation during the second cultivation phase is carried out at a second temperature, said second temperature being higher than the first temperature. The invention also provides for a Bacillus host cell culture obtainable by the said method.

    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING PARTIAL HARVEST
    6.
    发明公开
    INDUSTRIAL FERMENTATION PROCESS FOR BACILLUS USING PARTIAL HARVEST 审中-公开

    公开(公告)号：US20230272333A1

    公开(公告)日：2023-08-31

    申请号：US18018112

    申请日：2021-07-27

    Applicant: BASF SE

    Inventor： Andreas Daub ,  Aydin Golabgir Anbarani ,  Tobias Klein ,  Michael Morweiser ,  Georg Benjamin Wandrey

    IPC: C12N1/20

    CPC classification number: C12N1/205

    Abstract: The present invention relates to the field of industrial fermentation. In particular, it relates to method for producing a protein of interest from a Bacillus host cell comprising the steps of inoculating a fermentation medium with a Bacillus host cell comprising an expression construct for a gene encoding a protein of interest, cultivating the Bacillus host cell in said fermentation medium under conditions conducive for the growth of the Bacillus host cell and the expression of the protein of interest for a cultivation phase, wherein the cultivation of the Bacillus host cell during said cultivation phase comprises the addition of at least one feed solution and wherein the at least one feed solution provides a carbon source, and separating portions from said Bacillus host cell culture during the cultivation phase at different time points and obtaining the protein of interest from said portions.

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