公开(公告)号：US20140170665A1

公开(公告)日：2014-06-19

申请号：US14018205

申请日：2013-09-04

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： Amy L. Hiddessen ,  Erin Steenblock

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502784 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487

Abstract: This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir.

Abstract translation: 本公开提供了用于样品制备和/或分析的系统，装置和方法，其包括具有与载体流体储存器流体连通的第一通道和与样品储存器流体连通的第二通道的液滴发生器。 第一通道和第二通道可以在从样品储存器接收样品和来自载体流体储存器的载体流体的交叉点处相遇，并且产生沿着液滴通道流动到液滴储存器的一个或多个液滴。 与交叉点热连通的能量施加构件，液滴通道，样品储存器和/或载体流体储存器的至少一部分可以向一个或多个液滴的单个液滴（例如在交叉点处）提供能量，并且 /或当液滴沿着液滴通道移动到液滴储存器时。

公开(公告)号：US20140179544A1

公开(公告)日：2014-06-26

申请号：US14020742

申请日：2013-09-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Erin Steenblock ,  Amy L. Hiddessen ,  Ben Hindson ,  Kevin Ness

IPC: C12Q1/68

Abstract: The present disclosure provides assays and devices for forming, spacing, and/or detecting droplets. The droplets may be emulsions composed of two or more immiscible fluids. An emulsion can be a double emulsion, such as water-in-oil droplets that are present in a continuous aqueous phase. The double emulsion can be formed when the water-in-oil droplets are contacted with one or more streams of aqueous fluid(s). This disclosure also provides a variety of additives that can be added to the fluids.

Abstract translation: 本公开提供用于形成，间隔和/或检测液滴的测定和装置。 液滴可以是由两种或更多种不混溶流体组成的乳液。 乳液可以是双重乳液，例如存在于连续水相中的油包水油滴。 当油包水滴与一种或多种含水流体物流接触时，可以形成双重乳液。 本公开还提供了可以添加到流体中的各种添加剂。

公开(公告)号：US20140134623A1

公开(公告)日：2014-05-15

申请号：US13921455

申请日：2013-06-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy Hiddessen ,  Erin Steenblock

IPC: B01F5/00 ,  B01F9/00 ,  C12Q1/68 ,  B01F11/00

CPC classification number: C12Q1/6816 ,  B01F5/0085 ,  B01F5/0647 ,  B01F9/00 ,  B01F11/00 ,  B01F13/0062 ,  B01F13/0072 ,  B01F2215/0037 ,  B01L3/502784 ,  B01L3/52 ,  B01L2200/148 ,  B01L2200/185 ,  B01L2300/0867 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1861 ,  C12Q1/6806 ,  G01N1/38 ,  G01N21/6428 ,  G01N21/645 ,  G01N2001/2893 ,  G01N2021/6439 ,  G01N2201/127 ,  C12Q2563/159

Abstract: Provided herein, are droplet mixture compositions and systems and methods for forming mixtures of droplets. The system may comprise two or more droplet generation units. Each unit may include at least one first input well, a second input well, and an output well connected to the first and second input wells by channels that form a droplet generator. The combined droplet populations can be mixed, heated, and collected for multiple uses, such as for use as calibration standards for instrument testing and analysis.

Abstract translation: 本文提供了液滴混合物组合物和用于形成液滴混合物的系统和方法。 该系统可以包括两个或更多个液滴产生单元。 每个单元可以包括至少一个第一输入井，第二输入井和通过形成液滴发生器的通道连接到第一和第二输入井的输出井。 组合的液滴种群可以混合，加热和收集用于多种用途，例如用作仪器测试和分析的校准标准。