公开(公告)号：US12097495B2

公开(公告)日：2024-09-24

申请号：US17750195

申请日：2022-05-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, Jr. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  B01F25/00 ,  B01F25/23 ,  B01F33/302 ,  G01N35/08

CPC分类号： B01L3/50273 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/813 ,  B01L3/502 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  C12Q1/686 ,  B01F25/14 ,  B01F25/23 ,  B01F33/3021 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  G01N35/08

摘要： The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US12091710B2

公开(公告)日：2024-09-17

申请号：US17174519

申请日：2021-02-12

申请人： Bio-Rad Laboratories, Inc.

发明人： Darren Roy Link

IPC分类号： C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/14 ,  G01N35/00 ,  G01N35/08 ,  B01L7/00 ,  B01L9/00

CPC分类号： C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C2201/26 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

摘要： The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20240271226A1

公开(公告)日：2024-08-15

申请号：US18245048

申请日：2021-09-29

申请人： THE JOHNS HOPKINS UNIVERSITY

发明人： Tza-Huei Jeff WANG ,  Aniruddha Mrithinjay KAUSHIK ,  Kuangwen HSIEH

IPC分类号： C12Q1/689 ,  B01L3/00 ,  C12Q1/6818 ,  C12Q1/6897

CPC分类号： C12Q1/689 ,  B01L3/502784 ,  C12Q1/6818 ,  C12Q1/6897 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/0829 ,  B01L2300/0883

摘要： Provided herein are methods of identifying and determining the antimicrobial susceptibility of bacteria in samples. Related devices, systems, reaction mixtures, kits, and other methods are also provided.

公开(公告)号：US20240226886A1

公开(公告)日：2024-07-11

申请号：US18533164

申请日：2023-12-07

申请人： Dropworks, Inc.

发明人： Christopher Michael Perkins ,  Matthew Ryan Dunn ,  Andrew Carl Larsen ,  Donna Kelley ,  Michael Barich ,  Kristopher Holub ,  Pin Kao

IPC分类号： B01L3/00 ,  B01F23/41 ,  B01F25/314 ,  B01F33/302 ,  B01F101/16 ,  C12Q1/686 ,  G01N15/14 ,  G01N21/62 ,  G01N35/08

CPC分类号： B01L3/502715 ,  B01F23/41 ,  B01F25/314 ,  B01L3/502784 ,  C12Q1/686 ,  G01N15/1459 ,  G01N21/62 ,  B01F33/3021 ,  B01F2101/16 ,  B01L2200/0673 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0867 ,  G01N35/08

摘要： Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

公开(公告)号：US12030055B2

公开(公告)日：2024-07-09

申请号：US17708342

申请日：2022-03-30

申请人： LIGHTCAST DISCOVERY LTD

发明人： Thomas Henry Isaac ,  Pedro Cunha ,  Eoin Sheridan ,  David Love ,  Rebecca Palmer ,  Douglas J Kelly ,  Gareth Podd

IPC分类号： B01L3/00 ,  C12Q1/6869

CPC分类号： B01L3/502792 ,  C12Q1/6869 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/165 ,  B01L2300/168 ,  B01L2400/0427

摘要： A device comprising: a first zone comprising an attachment site; a first pathway; a second pathway and a means for creating a second medium comprised of aqueous microdroplets in a carrier; a microdroplet manipulation zone comprising: a first composite wall comprised of a first transparent substrate; a first transparent conductor layer on the substrate; a photoactive layer activated by electromagnetic radiation; and a first dielectric layer on the photoactive layer; a second composite wall comprised of a second substrate; a second conductor layer on the substrate; and optionally a second dielectric layer on the conductor layer; an A/C source; a source of first electromagnetic radiation; means for manipulating the points of impingement of the electromagnetic radiation on the photoactive layer; an detection zone disposed downstream of the microdroplet manipulation zone or integral therewith; and a fluorescence or Raman-scattering detection system.

公开(公告)号：US20240216915A1

公开(公告)日：2024-07-04

申请号：US18610088

申请日：2024-03-19

申请人： Roche Molecular Systems, Inc.

发明人： Michael Glauser ,  Daniel Muller ,  Michael Zeder ,  Uta Schaffner

IPC分类号： B01L3/00 ,  B01L7/00 ,  C12Q1/6806

CPC分类号： B01L3/502715 ,  C12Q1/6806 ,  B01L3/50851 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0605 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/0689 ,  B01L2300/042 ,  B01L2300/048 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0406 ,  B01L2400/0487 ,  B01L2400/0677

摘要： The present disclosure relates to reversible sealing of a microfluidic chip used for thermal incubation of an aqueous sample suspected to contain a target nucleic acid. The microfluidic chip contains a flow channel and a plurality of reaction compartments, into which the sample and a displacement fluid are introduced through an inlet port scalable by means of a sealing agent having a melting point above room temperature.

公开(公告)号：US12005454B2

公开(公告)日：2024-06-11

申请号：US17145858

申请日：2021-01-11

申请人： 10X Genomics, Inc.

发明人： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC分类号： B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC分类号： B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

摘要： The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US11998913B2

公开(公告)日：2024-06-04

申请号：US18120537

申请日：2023-03-13

申请人： Cellular Highways Ltd.

发明人： Mikhail Bashtanov ,  Richard Gold ,  Calum Hayes ,  Fred Hussain ,  Robyn Pritchard ,  Salman Samson Rogers ,  Nuno Varelas ,  Alexander Zhukov

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/149

CPC分类号： B01L3/502753 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L3/502776 ,  G01N15/1434 ,  B01L2200/0626 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/1805 ,  B01L2400/0442 ,  B01L2400/0487 ,  B01L2400/06 ,  G01N2015/1413 ,  G01N15/149

摘要： A single junction sorter for a microfluidic particle sorter, the single-junction sorter comprising: an input channel, configured to receive a fluid containing particles; an output sort channel and an output waste channel, each connected to the input channel for receiving the fluid therefrom; a bubble generator, operable to selectively displace the fluid around a particle to be sorted and thereby to create a transient flow of the fluid in the input channel; and a vortex element, configured to cause a vortex in the transient flow in order to direct the particle to be sorted into the output sort channel.

公开(公告)号：US20240165623A1

公开(公告)日：2024-05-23

申请号：US18281514

申请日：2022-03-11

申请人： Qatar Foundation for Education, Science and Community Development

发明人： Ali Sultan ,  Sini Skariah ,  Arum Han ,  Adrian Guzman

IPC分类号： B01L3/00 ,  G01N15/01 ,  G01N15/10

CPC分类号： B01L3/502784 ,  B01L3/502707 ,  G01N15/01 ,  G01N15/10 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/12 ,  G01N2015/1028

摘要： A method and an integrated device are provided for high-throughput screening of cellular libraries utilizing a droplet microfluidic-based approach. The integrated device comprises 8 or more major functionalities including droplet generation, droplet incubation, droplet reflow, droplet cleaving/generation, droplet synchronization, droplet merging, droplet detection, and droplet sorting for complex screening assays. Integration of each of the droplet functionalities onto a single chip reduces drastic changes in flow experienced at various chip-to-chip interfaces, and the possibility of error.

公开(公告)号：US20240139743A1

公开(公告)日：2024-05-02

申请号：US18281226

申请日：2022-03-08

申请人： NUCLERA LTD

发明人： Richard J. Paolini, Jr. ,  Seth Bishop

IPC分类号： B01L3/00

CPC分类号： B01L3/502792 ,  B01L2200/0673 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/165 ,  B01L2400/0427

摘要： Disclosed are methods and devices for sensing the presence of aqueous droplets on digital microfluidic devices.