公开(公告)号：US20150352547A1

公开(公告)日：2015-12-10

申请号：US14732682

申请日：2015-06-06

Applicant: Berkeley Lights, Inc.

Inventor： Keith Joseph Breinlinger ,  Eric D. Hobbs ,  Dorian Liepmann ,  Joshua Tanner Nevill ,  Mark P. White ,  Maria Jimena Loureiro

IPC: B01L3/00 ,  C12M3/06

CPC classification number: B01L3/5027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2400/086 ,  C12M23/16 ,  Y10T436/2575

Abstract: Some configurations of a microfluidic apparatus can comprise a fluidic circuit of interconnected fluidic structures into which a plurality of different media can be introduced or extracted. A variety of operations can be performed with the different media including isolating with a second medium one or more of the fluidic structures that is filled partially or fully with a first medium. Discrete volumes of a medium can be moved through the isolating second medium to deliver materials or micro-objects to or remove micro-objects or materials from a fluidic structure that is otherwise isolated by the second medium. Some configurations of a microfluidic apparatuses can isolate microfluidic structures in a microfluidic apparatus using flow rates or blocking structures, and some configurations can manage bubbles in fluidic structures.

Abstract translation: 微流体装置的一些构造可以包括互连流体结构的流体回路，多个不同介质可以被引入或提取到流体结构中。 可以使用不同的介质进行各种操作，包括用第二介质隔离一种或多种用第一介质部分或完全填充的流体结构。 介质的离散体积可以移动通过隔离的第二介质，以将材料或微物体输送到或从其被第二介质分离的流体结构中去除微物体或材料。 微流体装置的一些构造可以使用流速或阻塞结构隔离微流体装置中的微流体结构，并且一些配置可以管理流体结构中的气泡。