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公开(公告)号：US11305283B2
公开(公告)日：2022-04-19
申请号：US16509166
申请日：2019-07-11
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US20210368781A1
公开(公告)日：2021-12-02
申请号：US17228058
申请日：2021-04-12
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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公开(公告)号：US10751715B1
公开(公告)日：2020-08-25
申请号：US15136481
申请日：2016-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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公开(公告)号：US20200064337A1
公开(公告)日：2020-02-27
申请号：US16391063
申请日：2019-04-22
Applicant: Berkeley Lights, Inc.
Inventor： Minha Park , Jason C. Briggs , Jason M. McEwen , Ravi K. Ramenani , Hariharasudhan Chirra Dinakar , Kai W. Szeto , Adrienne T. Higa , Mark P. White , Randall D. Lowe, JR. , Xiaohua Wang , Kevin T. Chapman
IPC: G01N33/50 , B01L3/00 , C12N5/0781 , G01N33/68 , C12Q1/6876
Abstract: Methods are described herein for screening an antibody producing cell within a microfluidic environment. The antibody producing cell may be a B cell lymphocyte, which may be a memory B cell or a plasma cell. An antigen of interest may be brought into proximity with the antibody producing cell and binding of the antigen by an antibody produced by the antibody producing cell may be monitored. Methods of obtaining a sequencing library from an antibody producing cell are also described.
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公开(公告)号：US10376886B2
公开(公告)日：2019-08-13
申请号：US15843122
申请日：2017-12-15
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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Patent Agency Ranking
公开(公告)号：US09889445B2
公开(公告)日：2018-02-13
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M Jimena Loureiro
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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公开(公告)号：US09744533B2
公开(公告)日：2017-08-29
申请号：US14965721
申请日：2015-12-10
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
CPC classification number: B01L3/502761 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2300/0816 , B01L2300/0819 , B01L2300/0848 , B01L2300/0877 , B01L2400/0424 , B01L2400/0427 , B01L2400/0454 , B01L2400/0457 , B01L2400/086 , G01N1/4077 , G01N2001/4083
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
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公开(公告)号：US20150165436A1
公开(公告)日：2015-06-18
申请号：US14521447
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0668 , B01L2300/0636 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0454 , B03C5/005 , B03C5/026 , B03C2201/26 , G01N15/1484 , G01N33/5023 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54313 , G01N33/6854
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
Abstract translation: 在微流体装置中保持笔的生物活性可以通过放置在保持笔中捕获物体来测定，所述对象捕获物质与生物活性产生的特定感兴趣物质结合。 然后可以在微流体装置中或在从微流体装置输出捕获物体之后评估与每个捕获物体结合的感兴趣的生物材料。 该评估可用于表征每支持笔中的生物活性。 生物活性可以是生产感兴趣的生物材料。 因此，生物活性可以对应于或来自一个或多个生物细胞。 持有笔内的生物细胞可以是克隆细胞集落。 可以在保持每个菌落的克隆状态的同时测定每个克隆细胞集落的生物学活性。
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公开(公告)号：US12102083B2
公开(公告)日：2024-10-01
申请号：US17228058
申请日：2021-04-12
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, Jr.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , C12M47/04 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
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10.发明公开公开(公告)号：US20230347347A1
公开(公告)日：2023-11-02
申请号：US18068207
申请日：2022-12-19
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00
CPC classification number: B01L3/502761 , B01L2300/087 , B01L2300/0816 , B01L2200/0647 , B01L2200/0668 , B01L2300/0864 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , B01L2200/0652 , G01N2469/20
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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