公开(公告)号：US20240335832A1

公开(公告)日：2024-10-10

申请号：US18383385

申请日：2023-10-24

Applicant: InSilixa, Inc.

Inventor： Andrea Cuppoletti ,  Arjang Hassibi ,  Lei Pei ,  Yang Liu ,  Kshama Jirage ,  Arun Manickam

IPC: B01L3/00 ,  B01L7/00 ,  C07F7/08 ,  C23C16/02 ,  H01L27/146

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L3/502738 ,  B01L7/52 ,  C07F7/089 ,  C23C16/0272 ,  H01L27/14645 ,  B01L2200/0689 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2400/06

Abstract: The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.

公开(公告)号：US20240316557A1

公开(公告)日：2024-09-26

申请号：US18607538

申请日：2024-03-17

Applicant: Versitech Limited ,  Advanced Biomedical Instrumentation Centre Limited

Inventor： Jingxuan TIAN ,  Ho Cheung SHUM ,  Aditi DEY POONAM ,  Lang NAN

IPC: B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01L7/525 ,  B01L2200/0636 ,  B01L2200/0673 ,  B01L2200/147 ,  B01L2300/0883 ,  B01L2300/1822

Abstract: A portable, miniaturized microfluidic droplet-based digital polymerase chain reaction (PCR) device is provided. The device includes a droplet generation layer with a flow-focusing channel and multiple capillary channels, in which the flow-focusing channel has a flow focusing structure to generate droplets from a provided aqueous sample and extract oil, and the multiple capillary channels is configured to perform an oil extraction. An incubation layer includes heating plates, a microfluidic channel, and a container for conducting a PCR thermal cycling program. Controlled by a controller and powered by a power supplier, the PCR thermal cycling program involves maintaining predefined temperatures on each heating plate sequentially. This process ensures the heating of droplets to different temperatures at distinct times, facilitating precise and efficient PCR amplification.

公开(公告)号：US12064759B2

公开(公告)日：2024-08-20

申请号：US17245381

申请日：2021-04-30

Applicant: NUtech Ventures ,  UNIVERSITY OF CONNECTICUT

Inventor： Ruiguo Yang ,  Arian Jaberi ,  Amir Monemian Esfahani ,  Ali Tamayol

IPC: B01L3/00 ,  C12M1/34 ,  C12N1/34

CPC classification number: B01L3/5025 ,  C12M41/46 ,  C12N1/34 ,  B01L2300/087 ,  B01L2300/0877 ,  B01L2300/0883 ,  C12N2513/00

Abstract: Microfluidic gradient generators that can create robust platforms that can not only be used for creating co-cultures of cells with various ratios, but also can simultaneously generate gradients of mechanical and chemical stresses. A chip utilizes microchambers embedded within channels to provide space for 3D cell culture and exposes these cells to gradients of mechanical shear stress and a chemical treatment.

公开(公告)号：US12036554B2

公开(公告)日：2024-07-16

申请号：US17418868

申请日：2019-08-06

Applicant: MIKRO BIYOSISTEMLER ELEKTRONIK SANAYI VE TICARET A.S.

Inventor： Begum Sen Dogan ,  Ender Yildirim ,  Ozge Zorlu ,  Ebru Ozgur

IPC: B01L3/00 ,  G01N33/543 ,  G01N33/574

CPC classification number: B01L3/502761 ,  B01L3/502746 ,  B01L2200/0652 ,  B01L2200/12 ,  B01L2300/0848 ,  B01L2300/0883 ,  B01L2300/089 ,  G01N33/54366 ,  G01N33/574

Abstract: A microfluidic device is provided. The microfluidic device is used for an in vitro selective capture of biological entities suspended in a medium based on an immunoaffinity technique. The microfluidic device includes symmetric hydrofoil pillars arranged inside ellipse segments acting as a microfluidic channel, wherein the microfluidic channel provides a continuous change of attack angles between the symmetric hydrofoil pillars and the biological entities.

公开(公告)号：US12018319B2

公开(公告)日：2024-06-25

申请号：US16973990

申请日：2019-05-20

Applicant: HITACHI, LTD.

Inventor： Junko Tanaka ,  Tatsuo Nakagawa ,  Yuzuru Shimazaki ,  Kunio Harada

IPC: C12P19/34 ,  B01L3/00 ,  C12Q1/6825

CPC classification number: C12Q1/6825 ,  B01L3/5027 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0415 ,  B01L2400/0487 ,  C12Q2531/113 ,  C12Q2563/107

Abstract: The present invention addresses the problem of providing a novel digital PCR analysis method. One embodiment of the novel digital PCR analysis method is a DNA detection method including the steps of: partitioning a DNA solution containing a fluorescent-labeled probe or a DNA intercalator and a target DNA to be detected into a plurality of compartments; carrying out a nucleic acid amplification reaction in the compartments; measuring a fluorescence intensity in association with a temperature change; calculating a melting temperature of a DNA double strand based on a change in the fluorescence intensity in association with the temperature change; and calculating a ratio of a fluorescence intensity at a second temperature that is lower than a first temperature in association with the temperature change to that at the first temperature.

公开(公告)号：US20240198335A1

公开(公告)日：2024-06-20

申请号：US18286537

申请日：2022-04-08

Applicant: Swansea University

Inventor： Francesco Del Giudice ,  Keshvad Shahrivar

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2300/0867 ,  B01L2300/0883 ,  B01L2400/0487

Abstract: The invention relates to methods for separating particles in a microfluidic device and, ideally, encapsulating said particles in at least one or a stream of droplets; and a kit of parts for performing said methods.

公开(公告)号：US20240149267A1

公开(公告)日：2024-05-09

申请号：US18448562

申请日：2023-08-11

Applicant: The Board of Trustees of the Leland Stanford Junior University

Inventor： Mahdokht Masaeli ,  Euan A. Ashley

IPC: B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1434

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/50273 ,  G01N15/1404 ,  G01N15/1434 ,  G01N15/1484 ,  B01L2300/0883 ,  G01N2015/1006 ,  G01N2015/1445

Abstract: Systems and methods in accordance with various embodiments of the invention are capable of rapid analysis and classification of cellular samples based on cytomorphological properties. In several embodiments, cells suspended in a fluid medium are passed through a microfluidic channel, where they are focused to a single stream line and imaged continuously. In a number of embodiments, the microfluidic channel establishes flow that enables individual cells to each be imaged at multiple angles in a short amount of time. A pattern recognition system can analyze the data captured from high-speed images of cells flowing through this system and classify target cells. In this way, the automated platform creates new possibilities for a wide range of research and clinical applications such as (but not limited to) point of care services.

公开(公告)号：US20240139733A1

公开(公告)日：2024-05-02

申请号：US18568613

申请日：2022-06-08

Applicant: PharmaFluidics NV

Inventor： Gert DESMET ,  Paul JACOBS ,  Jeff OP DE BEECK ,  Bo CLAEREBOUT

IPC: B01L3/00

CPC classification number: B01L3/502707 ,  B01L2300/0883 ,  B01L2400/086

Abstract: A method (200) for producing a microfluidic apparatus (100) according to one or more design constraints. The method comprises: providing (210) a first substrate (110) comprising multiple fluid channels (120), wherein the channels have an inlet (123) and an outlet (124); implementing (220) a configuration of connecting channels (150) on at least one second substrate (140) depending on the specified design constraints of the microfluidic apparatus (100); such that by aligning (230) the first substrate with the at least one second substrate, at least some inlets and outlets of the fluid channels of the first substrate are connected to the connecting channels (150) and a microfluidic device (100) complying with the one or more design constraints is obtained.

公开(公告)号：US11952620B2

公开(公告)日：2024-04-09

申请号：US17095139

申请日：2020-11-11

Applicant: KABUSHIKI KAISHA TOSHIBA

Inventor： Koji Hashimoto ,  Keiko Ito ,  Mika Inada

IPC: C12Q1/6851 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/5027 ,  B01L3/502715 ,  B01L7/00 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/18 ,  C12Q1/6851 ,  C12Q2527/101 ,  C12Q2531/101 ,  C12Q2563/159 ,  C12Q2565/607

Abstract: According to one embodiment, a method of quantifying a target nucleic acid containing a first sequence in a sample is provided. The method includes preparing a substrate on which a plurality of detection regions are arranged, forming a reaction field by placing, on the substrate, a reaction liquid containing a sample, a primer set, and an amplification enzyme, maintaining the reaction field in an isothermal amplification condition, detecting a detection signal for each of the detection regions, determining, for each of the plurality of detection regions, whether positive or negative and detecting or quantifying the target nucleic acid based on the number of positive and/or a rise time of each of the positive detection signal.

公开(公告)号：US20240100524A1

公开(公告)日：2024-03-28

申请号：US18528671

申请日：2023-12-04

Applicant: mirOculus Inc.

Inventor： Mais J. JEBRAIL ,  Alexandra J. CHO ,  Victor LEE

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0883 ,  B01L2300/123 ,  B01L2300/165 ,  B01L2400/0427 ,  B01L2400/0487

Abstract: Microfluidic apparatuses, systems, devices and associated fluid manipulation and extraction devices, and methods of using them are presented. The devices may be useful for analysis of clinical, laboratory, chemical, or biological samples. A fluid application and extraction interface device may include a waste reservoir with a fluid trap extending above a waste chamber, an opening through the waste reservoir above the sample inlet, and a transfer conduit extending through the waste reservoir so that fluid may pass from the transfer conduit into the waste reservoir and be trapped within the waste chamber.