公开(公告)号：US20240361226A1

公开(公告)日：2024-10-31

申请号：US18765836

申请日：2024-07-08

Applicant: UNIVERSITY OF VIRGINIA PATENT FOUNDATION

Inventor： Nathan SWAMI ,  Walter VARHUE ,  Vahid FARMEHINI

IPC: G01N15/1031 ,  B01L3/00 ,  G01N15/01 ,  G01N33/483 ,  G01R27/16

CPC classification number: G01N15/1031 ,  B01L3/502715 ,  B01L3/502761 ,  G01N33/4833 ,  G01R27/16 ,  B01L2200/0647 ,  B01L2300/0627 ,  G01N15/01

Abstract: An exemplary method and system is disclosed that facilitate the integration of on-chip impedance sensors and measurement circuitries, e.g., in characterizing internal microfluidic structures and/or in cell or particle cytometry, for quantifying the impedance/frequency response of microfluidic device under the same, or similar, conditions used for particle manipulation. In some embodiments, the exemplary method and system employs a circuit configured for automated determination and quantification of parasitic voltage drops during AC electrokinetic particle manipulation, without the need to use valuable biological samples or model particles. The determined impedance response can be used to assess efficacy of the microfluidic device geometry as well as to provide control signals to inform downstream cell separation decisions.

公开(公告)号：US20240359180A1

公开(公告)日：2024-10-31

申请号：US18541650

申请日：2023-12-15

Applicant: Mammoth Biosciences, Inc.

Inventor： Timothy James PATNO ,  Phillip You Fai LEE ,  Benjamin Andrew BLIZARD ,  Xin MIAO ,  Jacob LESINSKI ,  Ryan A. BROWN ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO ,  Joshua BAIK ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Clare Louise FASCHING ,  Jesus CHING ,  Sonal JAIN ,  Deepika VERMA ,  Matthew VEROSLOFF ,  Devin SPRATT ,  Nicholas John FANTIN

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/161 ,  B01L2300/1805 ,  B01L2400/06

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

公开(公告)号：US20240351044A1

公开(公告)日：2024-10-24

申请号：US18577265

申请日：2022-07-08

Applicant: IXENSOR CO., LTD.

Inventor： Jiun-Yann YU ,  Yenyu CHEN

IPC: B01L7/00 ,  C12Q1/6851 ,  G01N21/64

CPC classification number: B01L7/52 ,  C12Q1/6851 ,  G01N21/6428 ,  B01L2200/0647 ,  B01L2300/1861 ,  B01L2300/1894 ,  G01N2021/6439 ,  G01N2201/0636

Abstract: Example methods and systems for performing quantitative polymerase chain reaction (qPCR) are provided. One example system may include a first lighting subsystem and a second lighting subsystem. The first lighting subsystem includes a first light source configured to illuminate a qPCR testing solution including a sample, a fluorescence dye and a nanoparticle in a tube with a first light to increase a temperature of the qPCR testing solution. The second lighting subsystem includes a light detector configured to detect an amount of fluorescence emitted by the fluorescence dye in a first thermal cycle of the qPCR. The first lighting subsystem is configured to turn off the first light source for a period of time in any thermal cycle of the qPCR after the first thermal cycle based on one or more heat transmission parameters which are determined based on the amount of fluorescence.

公开(公告)号：US12116569B2

公开(公告)日：2024-10-15

申请号：US18328085

申请日：2023-06-02

Applicant: Siemens Healthcare Diagnostics Inc.

Inventor： Alex Hofai Lee ,  Daniel Chu

IPC: B01F31/441 ,  B01F31/44 ,  B01F33/452 ,  B01F33/81 ,  B01F101/23 ,  B01L3/00 ,  B03C1/01 ,  B03C1/28 ,  C12N15/10

CPC classification number: C12N15/1013 ,  B01F31/441 ,  B01F31/449 ,  B01F33/452 ,  B01F33/813 ,  B01L3/5085 ,  B03C1/01 ,  B03C1/288 ,  B01F2101/23 ,  B01L2200/0647 ,  B03C2201/18 ,  B03C2201/26

Abstract: An apparatus, vortex generator assembly and method for automated cell lysis and nucleic acid purification and processing. The vortex generator assembly includes sample holder having a lysis well, at least one wash well, and an elution well. The vortex generator assembly also includes a sample holder cover having a plurality of vibration rods for creating a vortex in the wells of the sample holder. The apparatus includes motor operating a rotating cam to cause the vibration rods to vibrate and create the vortex in a well holding fluid and magnetic beads, wherein the vortexing speed is sufficient to overcome the magnetic attraction between the beads and disperse the beads in solution, to collect nucleic acids such as DNA.

公开(公告)号：US20240335837A1

公开(公告)日：2024-10-10

申请号：US18605476

申请日：2024-03-14

Applicant: TDK U.S.A. Corporation

Inventor： Manish Giri ,  Rakesh Sethi ,  Vadim Piskun

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0663 ,  B01L2300/0864 ,  B01L2400/0436 ,  B01L2400/0496

Abstract: A microfluidic device and a method for dissociating and manipulating cells or particles in a microfluidic channel are disclosed. The microfluidic device includes an inlet, an outlet, and a microfluidic channel arranged on a substrate between the inlet and the outlet. The microfluidic device includes a first set of piezoelectric actuators arranged adjacent to the inlet channel and configured to dissociate particles of a fluidic sample in the microfluidic channel. The microfluidic device includes a second set of piezoelectric actuators arranged between the inlet channel and the outlet channel and configured to manipulate the particles of the fluidic sample as the particles move through the microfluidic channel. The microfluidic device includes a third set of piezoelectric actuators arranged above the outlet channel, adjacent to the outlet, and configured to eject a portion of the fluidic sample out of the microfluidic channel via the outlet.

公开(公告)号：US12097495B2

公开(公告)日：2024-09-24

申请号：US17750195

申请日：2022-05-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, Jr. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  B01F25/00 ,  B01F25/23 ,  B01F33/302 ,  G01N35/08

CPC classification number: B01L3/50273 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/813 ,  B01L3/502 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  C12Q1/686 ,  B01F25/14 ,  B01F25/23 ,  B01F33/3021 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  G01N35/08

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US12091710B2

公开(公告)日：2024-09-17

申请号：US17174519

申请日：2021-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/14 ,  G01N35/00 ,  G01N35/08 ,  B01L7/00 ,  B01L9/00

CPC classification number: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C2201/26 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20240302318A1

公开(公告)日：2024-09-12

申请号：US18255896

申请日：2021-09-24

Applicant: University Of Cincinnati

Inventor： Jason Charles Heikenfeld

IPC: G01N27/327 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N27/3276 ,  B01L3/502761 ,  G01N27/3277 ,  G01N33/5438 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0645

Abstract: An aptamer sensing device is provided. The aptamer sensing device 200 includes at least one electrode 220. The aptamer sensing device further includes at least one binding feature 230. The aptamer sensing device further includes a plurality of aptamers 270 attached to the binding feature and not individually attached to the electrode, the aptamers further having an attached redox couple 272. The aptamer sensing device is configured to accept a sample fluid 242 including at least one analyte, and the binding feature is positioned relative to the electrode such that a binding of the analyte to the aptamers causes a shape change configuration in the binding feature that increases or decreases a charge transfer from the redox couple to the electrode.

公开(公告)号：US20240269670A1

公开(公告)日：2024-08-15

申请号：US18434614

申请日：2024-02-06

Applicant: IDEXX LABORATORIES, INC.

Inventor： Murthy Yerramilli ,  Jennifer Gagner ,  William Foster ,  Eugene Chan ,  Wayne Bell ,  Asante Malima

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/0689 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/123 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/0442

Abstract: A method for preparing analyzing a droplet on a surface of a cartridge, wherein the droplet comprises a plurality of particles is disclosed. The method includes transporting, via a plurality of electrodes of the cartridge, the droplet along a single path on the surface of the cartridge, wherein the single path is substantially linear and the plurality of electrodes is configured to transport the droplet along the single path on the surface of the cartridge. The method also includes analyzing the droplet at one or more locations along the single path on the surface of the cartridge.

公开(公告)号：US20240261786A1

公开(公告)日：2024-08-08

申请号：US18566208

申请日：2022-05-23

Applicant: Sartorius Stedim Biotech GmbH

Inventor： Alfons Kesting ,  Wei Sun ,  Marcel Bremerich ,  Louis Villain

IPC: B01L3/00 ,  B32B3/30 ,  B32B7/06 ,  B32B7/12 ,  B32B23/06 ,  B32B23/08 ,  B32B23/20 ,  B32B27/08 ,  B32B27/10 ,  B32B27/28 ,  B32B37/12 ,  B32B37/18 ,  B32B38/10

CPC classification number: B01L3/502761 ,  B32B3/30 ,  B32B7/06 ,  B32B7/12 ,  B32B23/06 ,  B32B23/08 ,  B32B23/20 ,  B32B27/08 ,  B32B27/10 ,  B32B27/286 ,  B32B37/12 ,  B32B37/182 ,  B32B38/10 ,  B01L2200/0647 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0851 ,  B01L2300/087 ,  B32B2250/03 ,  B32B2255/12 ,  B32B2255/26 ,  B32B2307/726 ,  B32B2307/7376 ,  B32B2307/748 ,  B32B2310/0843

Abstract: The invention relates to a product for providing membrane elements, comprising: a carrier film; and a membrane film, at least comprising: a support layer; and a membrane layer; the membrane film is releasably coupled to the carrier film; and the membrane film has a plurality of substantially discrete membrane elements. The invention also relates to a corresponding manufacturing process.