公开(公告)号：US20130288274A1

公开(公告)日：2013-10-31

申请号：US13692938

申请日：2012-12-03

Applicant: Bio Rad Laboratories, Inc.

Inventor： ROGER WALKER ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.

Abstract translation: 本发明提供了一种用于通过使来自受试者的体液样品与固定在固体支持物上的麦醇溶蛋白融合蛋白的六聚体形成的抗原来确定受试者是否患有乳糜泻的方法。 抗原的麦醇溶蛋白融合蛋白包括与诸如谷胱甘肽-S转移酶（GST）蛋白质的标签连接的重组脱酰胺麦醇溶蛋白。 通过将麦醇溶蛋白融合蛋白固定在固体支持物上来制备抗原。 抗原可以进一步包括与谷氨酸蛋白融合蛋白交联的组织转谷氨酰胺酶（tTG）。 当存在tTG时，将tTG和重组脱酰基麦醇溶蛋白在固定固相之前混合在一起。

公开(公告)号：US10921315B2

公开(公告)日：2021-02-16

申请号：US15617979

申请日：2017-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/531 ,  G01N33/564 ,  C07K14/415 ,  G01N33/68 ,  C12N9/10

Abstract: Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.

公开(公告)号：US20170307633A1

公开(公告)日：2017-10-26

申请号：US15617979

申请日：2017-06-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Roger Walker ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68 ,  C07K14/415 ,  G01N33/564 ,  C12N9/10

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: Methods of diagnosing celiac disease in a subject are provided. In some embodiments, the method comprises contacting a sample of bodily fluid from the subject with an antigen comprising a recombinant or synthetic deamidated gliadin protein, wherein the deamidated gliadin protein comprises a hexamer of peptides each having the sequence of SEQ ID NO:1; and detecting an antibody from the sample that specifically binds to the antigen, thereby diagnosing celiac disease in the subject.

公开(公告)号：US09702885B2

公开(公告)日：2017-07-11

申请号：US13692938

申请日：2012-12-03

Applicant: Bio Rad Laboratories, Inc.

Inventor： Roger Walker ,  Yabin Lu ,  Urvee Desai ,  Daming Shan

IPC: G01N33/68 ,  C12N9/10 ,  C07K14/415 ,  G01N33/564

CPC classification number: G01N33/6893 ,  C07K14/415 ,  C07K2319/21 ,  C07K2319/23 ,  C12N9/1044 ,  C12Y203/02013 ,  G01N33/564 ,  G01N2800/06

Abstract: The present invention provides a method for determining whether a subject is suffering from celiac disease by contacting a sample of bodily fluid from the subject, with an antigen formed from a hexamer of a gliadin fusion protein immobilized on a solid support. The gliadin fusion protein of the antigen includes a recombinant deamidated gliadin linked to a tag such as Glutathione-S transferase (GST) protein. The antigen is prepared by immobilizing the gliadin fusion protein on the solid support. The antigen can further include tissue Transglutaminase (tTG) cross-linked to the gliadin fusion protein. When tTG is present, the tTG and recombinant deamidated gliadin are mixed together prior to immobilization to the solid phase.