公开(公告)号：US20250121372A1

公开(公告)日：2025-04-17

申请号：US18901984

申请日：2024-09-30

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Christopher F. KELLY ,  Donald A. MASQUELIER

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01L3/02 ,  B01L7/00 ,  C12Q1/6806 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20210170412A1

公开(公告)日：2021-06-10

申请号：US17110095

申请日：2020-12-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Donald A. MASQUELIER ,  Billy W. COLSTON, JR. ,  Benjamin J. HINDSON

IPC: B01L3/00 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F3/08 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49

Abstract: Method of analysis. In the method, a first emulsion and a second emulsion substantially separated from one another by a spacer fluid may be formed. The first emulsion, the spacer fluid, and the second emulsion may be flowed in a channel from a fluid inlet to a fluid outlet of a heating and cooling station having two or more temperature-controlled zones, such that each emulsion is thermally cycled to promote amplification of a nucleic acid target in droplets of the emulsion. Amplification data may be collected from individual droplets of each emulsion downstream of the heating and cooling station. A level of the nucleic acid target present in each emulsion may be determined based on the amplification data collected from the individual droplets of the emulsion.

公开(公告)号：US20190247856A1

公开(公告)日：2019-08-15

申请号：US16397774

申请日：2019-04-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Christopher F. KELLY ,  Donald A. MASQUELIER

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  G01N35/08 ,  C12Q1/6806

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20210031200A1

公开(公告)日：2021-02-04

申请号：US16995555

申请日：2020-08-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Christopher F. KELLY ,  Donald A. MASQUELIER

IPC: B01L3/00 ,  B01F13/00 ,  B01F3/08 ,  G01N35/08 ,  C12Q1/6806

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20190255531A1

公开(公告)日：2019-08-22

申请号：US16404659

申请日：2019-05-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. HINDSON ,  Kevin D. NESS ,  Billy W. COLSTON, JR. ,  Fred P. MILANOVICH ,  Donald A. MASQUELIER

IPC: B01L3/00 ,  G01N21/64 ,  B01L7/00 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49 ,  B01F13/00 ,  B01L3/02 ,  G01N21/3563 ,  C12Q1/686 ,  B01F3/08

Abstract: Method of performing a droplet-based assay. The method may include obtaining droplets encapsulated by an immiscible liquid and packed closely together in a monolayer, performing a reaction in the droplets while packed closely together in the monolayer; and collecting data related to an analyte from a plurality of the droplets while the droplets remain closely packed together in the monolayer.

公开(公告)号：US20190046987A1

公开(公告)日：2019-02-14

申请号：US16160783

申请日：2018-10-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Christopher F. KELLY ,  Donald A. MASQUELIER

IPC: B01L3/00 ,  B01F3/08 ,  B01F13/00 ,  C12Q1/6806 ,  G01N35/08 ,  B01L7/00 ,  B01L3/02 ,  G01N35/10 ,  G01N35/00

Abstract: A system, including method and apparatus, for generating droplets suitable for droplet-based assays. The disclosed systems may include either one-piece or multi-piece droplet generation components configured to form sample-containing droplets by merging aqueous, sample-containing fluid with a background emulsion fluid such as oil, to form an emulsion of sample-containing droplets suspended in the background fluid. In some cases, the disclosed systems may include channels or other suitable mechanisms configured to transport the sample-containing droplets to an outlet region, so that subsequent assay steps may be performed.

公开(公告)号：US20210220829A1

公开(公告)日：2021-07-22

申请号：US17137274

申请日：2020-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin D. NESS ,  Donald A. MASQUELIER ,  Billy W. COLSTON, JR. ,  Benjamin J. HINDSON

IPC: B01L3/00 ,  B01F13/00 ,  B01L3/02 ,  B01L7/00 ,  G01N21/3563 ,  B01F3/08 ,  C12Q1/686 ,  G01N21/64 ,  B01F15/00 ,  B29C45/00 ,  G01N21/49

Abstract: System for performing a flow-based assay. The system may comprise a droplet generator to produce an emulsion including droplets in a carrier fluid. The system also may comprise a thermocycler including two or more temperature-controlled zones and also including a channel connected to the droplet generator for receiving the emulsion. The channel may form a single-pass continuous fluid route traversing the temperature-controlled zones multiple times, such that droplets passing through the channel are thermally cycled. The system further may comprise a detection station downstream from the thermocycler and configured to detect a signal from the droplets after such droplets have been thermally cycled by passing through the channel.

公开(公告)号：US20200086312A1

公开(公告)日：2020-03-19

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. HIDDESSEN ,  Donald A. MASQUELIER ,  Kevin D. NESS ,  Benjamin J. HINDSON ,  Anthony J. MAKAREWICZ, JR. ,  Erin R. CHIA ,  Billy W. COLSTON, JR. ,  Serge SAXONOV ,  Svilen S. TZONEV ,  Michael Y. LUCERO ,  Ryan T. KOEHLER

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  B01F3/08 ,  B01F13/10 ,  C12Q1/686

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.