公开(公告)号：US20240342705A1

公开(公告)日：2024-10-17

申请号：US18259645

申请日：2021-12-27

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY ,  NH FOODS LTD.

Inventor： Yusuke FUCHIWAKI ,  Masato TANAKA ,  Shohei YAMAMURA ,  Naoki MORISHITA ,  Seiichiro MATSUZAKI

IPC: B01L3/00 ,  G01N35/08

CPC classification number: B01L3/502707 ,  G01N35/08 ,  B01L2300/048 ,  B01L2300/069 ,  B01L2300/0867

Abstract: An assay device allows reducing manufacturing variation, maintaining the measurement accuracy of the assay device at a high level, and enhancing the liquid control performance. The assay device of the present invention includes a microfluidic channel 2 allowing liquid to flow, an absorbing porous medium 3 disposed at a distance from one end 2a of the microfluidic channel 2, a separating space 4 disposed between the microfluidic channel 2 and the absorbing porous medium 3, and a housing space 5 housing the absorbing porous medium 3. The assay device further includes a lower member 20 which is an integrally molded article constituting a part of the assay device. The lower member 20 defines a lower portion 2b of the microfluidic channel 2, a lower portion 4a of the separating space 4, and a lower portion 5a of the housing space 5. The lower portions 4a, 5a of the separating space 4 and the housing space 5 are inclined downward toward one side from the other side in a flow direction. The lower member 20 supports the absorbing porous medium 3 at the lower portion 5a of the housing space 5.

公开(公告)号：US12097495B2

公开(公告)日：2024-09-24

申请号：US17750195

申请日：2022-05-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Hindson ,  Serge Saxonov ,  Phillip Belgrader ,  Kevin D. Ness ,  Michael Y. Lucero ,  Billy W. Colston, Jr. ,  Shawn Paul Hodges ,  Nicholas J. Heredia ,  Jeffrey Clark Mellen ,  Camille Bodley Troup ,  Paul Wyatt

IPC: B01L3/00 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/81 ,  B01L9/00 ,  C12Q1/686 ,  B01F25/00 ,  B01F25/23 ,  B01F33/302 ,  G01N35/08

CPC classification number: B01L3/50273 ,  B01F23/41 ,  B01F33/3011 ,  B01F33/813 ,  B01L3/502 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  C12Q1/686 ,  B01F25/14 ,  B01F25/23 ,  B01F33/3021 ,  B01L3/502761 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  G01N35/08

Abstract: The present disclosure provides methods and compositions for detecting polynucleotides in a sample and for quantifying polynucleotide load in a sample. The polynucleotides can be associated with a disease, disorder, or condition. In some applications, methylated DNA is quantified, e.g., in order to determine the load of polynucleotides in a sample. The present disclosure also provides methods and compositions for determining the load of fetal polynucleotides in a biological sample, e.g., the load of fetal polynucleotides (e.g., DNA, RNA) in maternal plasma. The present disclosure provides methods and compositions for detecting cellular processes such as cellular viability, growth rates, and infection rates. This disclosure also provides compositions and methods for detecting differences in copy number of a target polynucleotide. In some embodiments, the methods and compositions provided herein are useful for diagnosis of fetal genetic abnormalities, when the starting sample is maternal tissue (e.g., blood, plasma). The methods and materials described apply techniques for allowing detection of small, but statistically significant, differences in polynucleotide copy number.

公开(公告)号：US12091710B2

公开(公告)日：2024-09-17

申请号：US17174519

申请日：2021-02-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  B03C5/00 ,  B03C5/02 ,  C12N15/10 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/14 ,  G01N35/00 ,  G01N35/08 ,  B01L7/00 ,  B01L9/00

CPC classification number: C12Q1/6848 ,  B01F23/41 ,  B01F33/3011 ,  B01J19/0093 ,  B01L3/502715 ,  B01L3/502753 ,  B01L3/502776 ,  B01L3/502784 ,  B01L3/565 ,  B03C5/005 ,  B03C5/026 ,  C12N15/1075 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N1/38 ,  G01N15/147 ,  G01N35/08 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/00853 ,  B01J2219/0086 ,  B01J2219/00869 ,  B01J2219/00889 ,  B01J2219/00903 ,  B01J2219/0097 ,  B01J2219/00988 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/165 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C2201/26 ,  C12Q2525/191 ,  C12Q2535/101 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/628 ,  C12Q2565/629 ,  G01N2035/00158 ,  G01N2035/00237 ,  G01N2035/00326

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel. The plurality of hollow members includes a first member configured to expel a fluid immiscible with droplets in the vessel and a second member configured to substantially only droplets from the vessel. The assembly also includes a main channel, in which the second member is in fluid communication with the main channel. The assembly also includes at least one analysis module connected to the main channel.

公开(公告)号：US20240264194A1

公开(公告)日：2024-08-08

申请号：US18432494

申请日：2024-02-05

Applicant: Samsung Electronics Co., Ltd.

Inventor： Yeontae Kim ,  Jinhee Park ,  Joengseok Kim ,  Jinseop Lee ,  Bongkyun Jin ,  Eunju Park ,  Eungsun Lee ,  Hyekyoung Moon ,  Hyangbong Lee ,  Hyunyul Park ,  Seoungkyo Yoo

IPC: G01N35/10 ,  G01N33/18 ,  G01N35/08

CPC classification number: G01N35/1095 ,  G01N33/1813 ,  G01N35/085 ,  G01N35/1016

Abstract: Provided is a contaminant analysis apparatus including a pretreatment sampler configured to collect and filter an effluent discharged through a discharge pipe to provide an analysis target sample, a sample introduction unit configured to receive the analysis target sample from the pretreatment sampler, a sample injection unit configured to selectively supply the analysis target sample supplied from the sample introduction unit, a sample analysis unit including an integrated analyzer configured to analyze ion components and heavy metal components of the analysis target sample which is supplied from the sample injection unit, and an analysis controller configured to control the sample introduction unit, the sample injection unit, and the sample analysis unit, wherein the sample introduction unit includes a first sample introduction unit including two or more first sample introduction unit syringe pumps and a second sample introduction unit including one or more second sample introduction unit syringe pumps.

公开(公告)号：US12050231B2

公开(公告)日：2024-07-30

申请号：US17615893

申请日：2020-05-25

Applicant: Shimadzu Corporation

Inventor： Hiroshi Iwata ,  Hiroomi Goto ,  Shigehiko Zoda ,  Eiichi Ozeki ,  Takahiro Nishimoto ,  Aline Rotzetter ,  Yasuko Yoneda ,  Ippei Takeuchi

IPC: G01N35/08 ,  G02B21/36

CPC classification number: G01N35/085 ,  G02B21/365

Abstract: A testing method using a micro flow path device configured for a test liquid containing a specimen to be brought into contact with a drug therein and for a test on an action of the drug on the specimen includes: preparing the micro flow path device including: a plurality of micro flow paths, first and second openings which are disposed at both ends of each of the plurality of micro flow paths and communicate with an outside, a storage unit which is provided in each of the plurality of micro flow paths and stores the drug, and a gas-permeable membrane covering the first opening; applying a fluid pressure higher than an external pressure to the test liquid through the second opening from a syringe pump connected to the second opening to pressure-feed the test liquid to the storage unit; and observing a target region set in the micro flow path.

公开(公告)号：US20240226886A1

公开(公告)日：2024-07-11

申请号：US18533164

申请日：2023-12-07

Applicant: Dropworks, Inc.

Inventor： Christopher Michael Perkins ,  Matthew Ryan Dunn ,  Andrew Carl Larsen ,  Donna Kelley ,  Michael Barich ,  Kristopher Holub ,  Pin Kao

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/314 ,  B01F33/302 ,  B01F101/16 ,  C12Q1/686 ,  G01N15/14 ,  G01N21/62 ,  G01N35/08

CPC classification number: B01L3/502715 ,  B01F23/41 ,  B01F25/314 ,  B01L3/502784 ,  C12Q1/686 ,  G01N15/1459 ,  G01N21/62 ,  B01F33/3021 ,  B01F2101/16 ,  B01L2200/0673 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0867 ,  G01N35/08

Abstract: Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

公开(公告)号：US12005454B2

公开(公告)日：2024-06-11

申请号：US17145858

申请日：2021-01-11

Applicant: 10X Genomics, Inc.

Inventor： Rajiv Bharadwaj ,  Kevin Ness ,  Debkishore Mitra ,  Donald A. Masquelier ,  Anthony Makarewicz ,  Christopher Hindson ,  Benjamin Hindson ,  Serge Saxonov

IPC: B01L3/00 ,  B01L3/02 ,  G01N35/00 ,  G01N35/08 ,  G01N35/10

CPC classification number: B01L3/502761 ,  B01L3/0241 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2400/049 ,  B01L2400/086 ,  G01N2035/00237 ,  G01N35/085 ,  G01N2035/1034

Abstract: The disclosure provides devices, systems and methods for the generation of encapsulated reagents and the partitioning of encapsulated reagents for use in subsequent analyses and/or processing, such as in the field of biological analyses and characterization.

公开(公告)号：US11931736B2

公开(公告)日：2024-03-19

申请号：US17062040

申请日：2020-10-02

Applicant: Dropworks, Inc.

Inventor： Christopher Michael Perkins ,  Matthew Ryan Dunn ,  Andrew Carl Larsen ,  Donna Kelley ,  Michael Barich ,  Kristopher Holub ,  Pin Kao

IPC: B01L3/00 ,  B01F23/41 ,  B01F25/314 ,  C12Q1/686 ,  G01N15/14 ,  G01N21/62 ,  B01F33/302 ,  B01F101/16 ,  G01N35/08

CPC classification number: B01L3/502715 ,  B01F23/41 ,  B01F25/314 ,  B01L3/502784 ,  C12Q1/686 ,  G01N15/1459 ,  G01N21/62 ,  B01F33/3021 ,  B01F2101/16 ,  B01L2200/0673 ,  B01L2300/041 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/0867 ,  G01N35/08

Abstract: Disclosed herein are systems and methods for serial flow emulsion processes. Systems and methods as described herein result in reduced cross-contamination.

公开(公告)号：US20240085443A1

公开(公告)日：2024-03-14

申请号：US18273043

申请日：2021-01-20

Applicant: Enplas Corporation

Inventor： Nobuya SUNAGA

IPC: G01N35/08 ,  G01N35/10

CPC classification number: G01N35/08 ,  G01N35/1065

Abstract: This liquid handling device comprises a first flow path, a second flow path, a third flow path, an introduction opening, a discharge opening, an introduction valve, and a discharge valve. The third flow path comprises a first area under detection that is disposed in the third flow path and includes a roughened surface so as to have light emitted thereon for the purpose of transmitted light or reflected light detection and a second area under detection that is disposed further to one side of the third flow path than the first area under detection and includes a roughened surface so as to have light emitted thereon for the purpose of transmitted light or reflected light detection.

公开(公告)号：US11828768B2

公开(公告)日：2023-11-28

申请号：US16638327

申请日：2018-10-25

Applicant: LG Chem, Ltd.

Inventor： Byung Hyun Park ,  Byoung Hyoun Kim ,  Dong Hyun Kim ,  Su Youn Han

IPC: G01N21/78 ,  G01N21/75 ,  G01N21/77 ,  G01N31/22 ,  G01N35/10 ,  G01N35/08 ,  B01L3/00 ,  G01N33/20

CPC classification number: G01N35/1095 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502715 ,  G01N21/78 ,  G01N31/22 ,  G01N33/20 ,  G01N35/08 ,  B01L2200/16 ,  B01L2300/06 ,  B01L2300/0803 ,  B01L2300/126 ,  B01L2300/16 ,  B01L2400/0475

Abstract: The present invention relates to a device and a method for qualitative and quantitative analysis of heavy metals and more particularly provides a device and a method for qualitative and quantitative analysis of heavy metals utilizing a rotary disc system.