公开(公告)号：US20200086312A1

公开(公告)日：2020-03-19

申请号：US16667811

申请日：2019-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. HIDDESSEN ,  Donald A. MASQUELIER ,  Kevin D. NESS ,  Benjamin J. HINDSON ,  Anthony J. MAKAREWICZ, JR. ,  Erin R. CHIA ,  Billy W. COLSTON, JR. ,  Serge SAXONOV ,  Svilen S. TZONEV ,  Michael Y. LUCERO ,  Ryan T. KOEHLER

IPC: B01L3/00 ,  B01L9/00 ,  B01F13/00 ,  B01F3/08 ,  B01F13/10 ,  C12Q1/686

Abstract: Method of detecting a target nucleic acid. In an exemplary method, at least two thermal zones of different temperature may be created using a heating assembly. A first emulsion and a second emulsion may be formed. The first and second emulsions may be thermally cycled by passing them through tubing in a spaced relation to one another, with the tubing being wound around a central axis of the heating assembly and extending through each thermal zone multiple times. Thermally cycling may promote amplification of the target nucleic acid in droplets of each emulsion. Droplets of each emulsion may be passed through a detection channel located downstream of the tubing. Fluorescence may be detected from the droplets being passed through the detection channel.

公开(公告)号：US20230070234A1

公开(公告)日：2023-03-09

申请号：US17986873

申请日：2022-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6851 ,  B01F23/41

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US20190002956A1

公开(公告)日：2019-01-03

申请号：US16022500

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： David P. STUMBO ,  George CARMAN ,  Steve HOBBS ,  Anthony J. MAKAREWICZ, JR. ,  Dmitri SIMONIAN ,  David GLADE ,  Joshua OEN ,  Denis PRISTINSKI ,  John DZENITIS

IPC: C12Q1/686 ,  B01L3/00 ,  G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844

Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.