公开(公告)号：US20230086701A1

公开(公告)日：2023-03-23

申请号：US17946555

申请日：2022-09-16

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Evan THRUSH ,  Stephen SWIHART ,  Kevin McDONALD

IPC: G01N21/64 ,  G06T5/50 ,  G06V10/60 ,  G01N21/76 ,  G01N33/68

Abstract: Aspects of the present disclosure relate to systems and methods for generating a composite image. This can include a western blot imager with a real time camera. One aspect of the present disclosure relates to an imaging system. The imaging system includes a sample plane that can receive and hold a sample, a photon resolving camera, and a lens attached to the photon resolving camera, the photon resolving camera and the lens positioned to image the sample plane.

公开(公告)号：US20190178877A1

公开(公告)日：2019-06-13

申请号：US16158853

申请日：2018-10-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Steve FREEBY ,  Ning LIU ,  Kevin McDONALD ,  Aran PAULUS ,  Anton POSCH

IPC: G01N33/53 ,  G16B99/00 ,  G06T11/60 ,  G01N33/68 ,  G06T7/00

CPC classification number: G01N33/53 ,  G01N33/6827 ,  G06T7/0012 ,  G06T11/60 ,  G06T2207/30004 ,  G16B99/00

Abstract: Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

公开(公告)号：US20240302323A1

公开(公告)日：2024-09-12

申请号：US18594870

申请日：2024-03-04

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Kevin McDONALD ,  Evan THRUSH ,  Stephen SWIHART

IPC: G01N27/447 ,  H04N23/56

CPC classification number: G01N27/44721 ,  G01N27/44739 ,  H04N23/56

Abstract: Systems, methods, and devices for imaging of stain-free fluorescence on western blot membranes with excitation by epi illumination with UV LEDs are disclosed herein. A method can include collecting and preparing a sample, separating the sample via gel electrophoresis in a gel block, transferring the separated sample from the gel block to an analysis block, generating an image of the sample with an imager, and evaluating the image of the sample. The imager can include a plane to receive and hold a block containing a sample and including a first side and a second side, a camera to image a sample on the plane and positioned above the first side of the plane, and an LED light source positioned above the first side of the plane. The LED light source can illuminate the sample on the plane via epi-illumination, and emits light having a wavelength in a range from approximately 325 nm to approximately 400 nm.