公开(公告)号：US20230210926A1

公开(公告)日：2023-07-06

申请号：US18066814

申请日：2022-12-15

Applicant: California Institute of Technology

Inventor： Marjorie T. Buss ,  Robert C. Hurt ,  Katie K. Wong ,  Mikhail G. Shapiro ,  Mengtong Duan ,  Arash Farhadi ,  Mei Yi You

IPC: A61K35/748 ,  C07K14/195 ,  A61K49/22 ,  A61P35/00

CPC classification number: A61K35/748 ,  A61K49/22 ,  A61P35/00 ,  C07K14/195 ,  A61K2035/115

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in dynamic non-destructive imaging. The non-destructive imaging can be nonlinear ultrasound imaging. There are provided, in some embodiments, nucleic acid compositions encoding gas vesicles (GVs) capable of producing nonlinear ultrasound contrast upon expression in a prokaryotic cell (e.g., a probiotic bacterial cell) or a eukaryotic cell (e.g., a therapeutic mammalian cell).

公开(公告)号：US11761008B2

公开(公告)日：2023-09-19

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20230016245A1

公开(公告)日：2023-01-19

申请号：US17866240

申请日：2022-07-15

Applicant: California Institute of Technology

Inventor： Mengtong Duan ,  Mikhail G. Shapiro

IPC: C12N15/85 ,  A61K49/22

Abstract: Disclosed herein include methods, compositions, and kits enabling expression of multiple proteins from a single mRNA with a predetermined stoichiometry. There are provided, in some embodiments, nucleic acid compositions comprising a promoter operably linked to a polynucleotide comprising a first nucleic acid unit encoding first unit payload protein(s) and a second nucleic acid unit encoding second unit payload protein(s). The first nucleic acid unit and the second nucleic acid unit can each comprise a 3′ engineered translation initiation site (eTIS) comprising a three-nucleotide tunable element immediately upstream of a start codon. The eTIS of each of the first nucleic acid unit and the second nucleic acid unit can be configured to achieve a predetermined stoichiometry of the first unit payload protein(s) and the second unit payload protein(s) in a cell or cell-like environment.

公开(公告)号：US12241069B2

公开(公告)日：2025-03-04

申请号：US18317915

申请日：2023-05-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US20240366793A1

公开(公告)日：2024-11-07

申请号：US18653868

申请日：2024-05-02

Applicant: California Institute of Technology

Inventor： Ishaan J. Dev ,  Mengtong Duan ,  Mikhail G. Shapiro

IPC: A61K48/00 ,  C12N15/11 ,  C12N15/85

Abstract: Disclosed herein include methods, compositions, and kits enabling expression of multiple payload proteins from a single mRNA configured to achieve a predetermined stoichiometry of first unit payload protein(s) and second unit payload protein(s) in a cell or cell-like environment. There are provided, in some embodiments, nucleic acid compositions comprising a polynucleotide comprising a first nucleic acid unit (encoding one or more first unit payload protein(s)) and a second nucleic acid unit (encoding one or more second unit payload protein(s)). The first nucleic acid unit and the second nucleic acid unit can each comprise an engineered translation initiation site (eTIS) comprising a three-nucleotide tunable element immediately upstream of a start codon. In some embodiments, the nucleic acid composition comprises at least one modified nucleotide and/or at least one nucleotide analogue or nucleotide derivative. In some embodiments, the polynucleotide comprises a disclosed eTIS combination.

公开(公告)号：US11649462B2

公开(公告)日：2023-05-16

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.