公开(公告)号：US20230210926A1

公开(公告)日：2023-07-06

申请号：US18066814

申请日：2022-12-15

Applicant: California Institute of Technology

Inventor： Marjorie T. Buss ,  Robert C. Hurt ,  Katie K. Wong ,  Mikhail G. Shapiro ,  Mengtong Duan ,  Arash Farhadi ,  Mei Yi You

IPC: A61K35/748 ,  C07K14/195 ,  A61K49/22 ,  A61P35/00

CPC classification number: A61K35/748 ,  A61K49/22 ,  A61P35/00 ,  C07K14/195 ,  A61K2035/115

Abstract: Disclosed herein include methods, compositions, and kits suitable for use in dynamic non-destructive imaging. The non-destructive imaging can be nonlinear ultrasound imaging. There are provided, in some embodiments, nucleic acid compositions encoding gas vesicles (GVs) capable of producing nonlinear ultrasound contrast upon expression in a prokaryotic cell (e.g., a probiotic bacterial cell) or a eukaryotic cell (e.g., a therapeutic mammalian cell).

公开(公告)号：US11761008B2

公开(公告)日：2023-09-19

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US12241069B2

公开(公告)日：2025-03-04

申请号：US18317915

申请日：2023-05-15

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.

公开(公告)号：US11649462B2

公开(公告)日：2023-05-16

申请号：US16736683

申请日：2020-01-07

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Arash Farhadi ,  Gabrielle H Ho ,  Daniel P Sawyer ,  Mikhail Shapiro ,  Robert C. Hurt ,  Mengtong Duan

IPC: C12N15/67 ,  C07K14/195

CPC classification number: C12N15/67 ,  C07K14/195 ,  C12N2510/02

Abstract: Provided herein are genetically engineered gas vesicle expression systems (GVES) that are configured to express gas vesicles (GVs) in a mammalian cell, related gas vesicle polynucleotide constructs, gas vesicle reporting genetic circuits, vectors, genetically engineered mammalian cells, non-human mammalian hosts, compositions, methods and systems, which in several embodiments can be used together with contrast-enhanced imaging techniques to detect and report biological events in an imaging target site comprising a mammalian cell and/or organism.