公开(公告)号：US20230055359A1

公开(公告)日：2023-02-23

申请号：US17821093

申请日：2022-08-19

Applicant: Cepheid

Inventor： Amish Shah ,  Marissa Lee ,  Jessica Koay ,  Richard J Casler, JR.

IPC: G01R31/26 ,  G01N21/76 ,  B01L7/00 ,  G01N35/00

Abstract: Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods includes measuring a voltage across the LED, and correlating the voltage to a junction temperature of the LED. This correlation can be used to improve operation of the LED by increasing the signal to noise ratio of the LED signal, characterize the LED by comparing to an I-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED. Improved performance of the LED can include stabilizing the photon output during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output during the assay. System and device with control units configured to perform these methods are also described herein.

公开(公告)号：US20220253079A1

公开(公告)日：2022-08-11

申请号：US17574951

申请日：2022-01-13

Applicant: Cepheid

Inventor： Marissa Lee ,  Jessica Koay ,  Charles Rohrs ,  Earl Solis ,  Matthew Piccini ,  Richard J. Casler, JR.

IPC: G05D23/19 ,  F25B21/02 ,  G01K1/02 ,  G01K13/024 ,  G01N1/42 ,  G01N1/44 ,  B01L7/00 ,  B01L1/02

Abstract: Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.