公开(公告)号：US09163225B2

公开(公告)日：2015-10-20

申请号：US14141890

申请日：2013-12-27

Applicant: Dow AgroSciences LLC

Inventor： James M. Hasler ,  Jianquan Li ,  Joel J. Sheets

IPC: C12N9/48 ,  C07K14/435

CPC classification number: C12N9/485 ,  C07K14/43563 ,  C07K2319/21

Abstract: The invention is drawn to a method for purifying membrane-bound proteins expressed in recombinant insect cells using N-laurosarcosine. The invention is particularly suited for expressing cadherin-type receptors cloned from Ostrinia nubilalis, European Corn Borer, and expressed in Sf9 insect cells. The method is optionally adapted for use with 6-his tag proteins.

Abstract translation: 本发明涉及用重组昆虫细胞中使用N-月桂肌苷来纯化膜结合蛋白的方法。 本发明特别适用于表达从Ostrinia nubilalis（欧洲玉米螟）克隆的钙粘蛋白型受体，并在Sf9昆虫细胞中表达。 该方法任选适于与6-his标签蛋白一起使用。

公开(公告)号：US10570182B2

公开(公告)日：2020-02-25

申请号：US15541303

申请日：2015-12-16

Applicant: DOW AGROSCIENCES LLC

Inventor： Joel J. Sheets ,  Kenneth Narva ,  Thomas Meade ,  Timothy D. Hey ,  Sek Yee Tan ,  Audrey Jane Etter ,  Todd P. Glancy ,  Janna Mai Armstrong ,  Tristan E. Coram ,  Krishna M. Madduri ,  James E. King ,  Ryan M. Lee ,  Gaofeng Lin ,  Jianquan Li

IPC: C12N15/82 ,  C07K14/325 ,  A01N63/02 ,  A01N37/46

Abstract: The subject invention concerns Bacillus thuringiensis modified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

公开(公告)号：US20190330292A1

公开(公告)日：2019-10-31

申请号：US16401246

申请日：2019-05-02

Applicant: DOW AGROSCIENCES, LLC

Inventor： Joseph Petolino ,  Jianquan Li ,  Steven L. Evans ,  Ryan C. Blue

IPC: C07K14/47 ,  C07K14/415 ,  C12N15/82 ,  C12N15/62

Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

公开(公告)号：US20210087241A1

公开(公告)日：2021-03-25

申请号：US17073831

申请日：2020-10-19

Applicant: Dow AgroSciences LLC

Inventor： Joseph F. Petolino ,  Jianquan Li ,  Steven L. Evans ,  Ryan C. Blue

IPC: C07K14/47 ,  C12N15/62 ,  C07K14/415 ,  C12N15/82

Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

公开(公告)号：US10351610B2

公开(公告)日：2019-07-16

申请号：US13757674

申请日：2013-02-01

Applicant: Dow AgroSciences LLC

Inventor： Joseph Petolino ,  Jianquan Li ,  Steven L Evans ,  Ryan C Blue

IPC: C12N15/82 ,  C12N15/113 ,  C07K14/47 ,  C12N15/62 ,  C07K14/415

Abstract: This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.