公开(公告)号：US10800819B2

公开(公告)日：2020-10-13

申请号：US15824372

申请日：2017-11-28

申请人： Dow AgroSciences LLC

发明人： Janna Mai Armstrong ,  Audrey Jane Etter ,  Meghan L. Frey ,  Premchand Gandra ,  Ted Letherer ,  Gaofeng Lin ,  Krishna M. Madduri ,  Haley Mowery ,  Kenneth E. Narva ,  Joel J. Sheets ,  Sek Yee Tan

IPC分类号： C12N15/82 ,  C07K14/32 ,  A01N37/46 ,  C07K14/325 ,  A01N63/10

摘要： DIG-657 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes DIG-657 variants, fragments and analogs.

公开(公告)号：US20160237452A1

公开(公告)日：2016-08-18

申请号：US15047456

申请日：2016-02-18

申请人： Dow AgroSciences LLC

发明人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Aaron T. Woosley ,  Stephanie L. Burton

IPC分类号： C12N15/82 ,  A01N63/02

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  Y02A40/162

摘要： The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

公开(公告)号：US09163225B2

公开(公告)日：2015-10-20

申请号：US14141890

申请日：2013-12-27

申请人： Dow AgroSciences LLC

发明人： James M. Hasler ,  Jianquan Li ,  Joel J. Sheets

IPC分类号： C12N9/48 ,  C07K14/435

CPC分类号： C12N9/485 ,  C07K14/43563 ,  C07K2319/21

摘要： The invention is drawn to a method for purifying membrane-bound proteins expressed in recombinant insect cells using N-laurosarcosine. The invention is particularly suited for expressing cadherin-type receptors cloned from Ostrinia nubilalis, European Corn Borer, and expressed in Sf9 insect cells. The method is optionally adapted for use with 6-his tag proteins.

摘要翻译： 本发明涉及用重组昆虫细胞中使用N-月桂肌苷来纯化膜结合蛋白的方法。 本发明特别适用于表达从Ostrinia nubilalis（欧洲玉米螟）克隆的钙粘蛋白型受体，并在Sf9昆虫细胞中表达。 该方法任选适于与6-his标签蛋白一起使用。

公开(公告)号：US10119149B2

公开(公告)日：2018-11-06

申请号：US15047456

申请日：2016-02-18

申请人： Dow AgroSciences LLC

发明人： Thomas Meade ,  Kenneth Narva ,  Nicholas P. Storer ,  Joel J. Sheets ,  Aaron T. Woosley ,  Stephanie L. Burton

IPC分类号： C12N15/82 ,  A01H5/10 ,  C07K14/325 ,  C12N15/32 ,  A01N63/02

摘要： The subject invention includes methods and plants for controlling European corn borer, said plants comprising a Cry1Ab insecticidal protein and a DIG-3 insecticidal protein to delay or prevent development of resistance by the insect.

公开(公告)号：US09879055B2

公开(公告)日：2018-01-30

申请号：US14740326

申请日：2015-06-16

申请人： Dow AgroSciences LLC

发明人： Janna Mai Armstrong ,  Audrey Jane Etter ,  Meghan L. F. Frey ,  Premchand Gandra ,  Ted Letherer ,  Gaofeng Lin ,  Krishna M. Madduri ,  Haley R. Mowery ,  Kenneth Narva ,  Joel J. Sheets ,  Sek Yee Tan

IPC分类号： C12N15/82 ,  C07K14/32 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325

CPC分类号： C07K14/32 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： DIG-657 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes DIG-657 variants, fragments and analogs.

公开(公告)号：US20180086795A1

公开(公告)日：2018-03-29

申请号：US15824372

申请日：2017-11-28

申请人： Dow AgroSciences LLC

发明人： Janna Mai Armstrong ,  Audrey Jane Etter ,  Meghan L. Frey ,  Premchand Gandra ,  Ted Letherer ,  Gaofeng Lin ,  Krishna M. Madduri ,  Haley Mowery ,  Kenneth E. Narva ,  Joel J. Sheets ,  Sek Yee Tan

IPC分类号： C07K14/32 ,  A01N63/02 ,  A01N37/46 ,  C12N15/82 ,  C07K14/325

摘要： DIG-657 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes DIG-657 variants, fragments and analogs.

公开(公告)号：US20170298381A1

公开(公告)日：2017-10-19

申请号：US15485353

申请日：2017-04-12

申请人： Dow AgroSciences LLC

发明人： Kenneth E. Narva ,  Joel J. Sheets ,  Sek Yee Tan ,  Vimbai Chikwana

IPC分类号： C12N15/82

CPC分类号： C12N15/8286 ,  A01N37/46 ,  A01N65/00 ,  Y02A40/162

摘要： The subject invention includes methods and plants for controlling lepidopteran pests, especially soybean looper (Pseudoplusia includens) and velvet bean caterpillar (Anticarsia gemmatalis) insects. The plants, preferably soybean plants, comprise a combination of Cry1Ca, Cry1Ea, Cry2Aa, and Vip3Ab1 insecticidal proteins. Methods are described for using the plants to delay or prevent the development of resistance by insects.

公开(公告)号：US20140109263A1

公开(公告)日：2014-04-17

申请号：US14047205

申请日：2013-10-07

申请人： Dow Agrosciences LLC

发明人： Joel J. Sheets ,  Kenneth E. Narva ,  Stephanie Burton ,  Elizabeth A. Caldwell

IPC分类号： A01N63/02 ,  C12N15/82

CPC分类号： C07K14/325 ,  A01N63/02 ,  C12N15/8286 ,  Y02A40/162

摘要： The subject invention includes methods and plants for controlling fall armyworm lepidopteran insects, said plants comprising a Cry1Ea insecticidal protein and a second insecticidal protein selected from the group of Cry1Ab, Cry1Be, Cry1Ca, Cry1Da, and Vip3Ab to delay or prevent development of resistance by the insects.

摘要翻译： 本发明包括用于控制秋季粘虫鳞翅目昆虫的方法和植物，所述植物包含Cry1Ea杀虫蛋白和选自Cry1Ab，Cry1Be，Cry1Ca，Cry1Da和Vip3Ab的第二种杀虫蛋白，以延迟或防止由 昆虫。

公开(公告)号：US10570182B2

公开(公告)日：2020-02-25

申请号：US15541303

申请日：2015-12-16

申请人： DOW AGROSCIENCES LLC

发明人： Joel J. Sheets ,  Kenneth Narva ,  Thomas Meade ,  Timothy D. Hey ,  Sek Yee Tan ,  Audrey Jane Etter ,  Todd P. Glancy ,  Janna Mai Armstrong ,  Tristan E. Coram ,  Krishna M. Madduri ,  James E. King ,  Ryan M. Lee ,  Gaofeng Lin ,  Jianquan Li

IPC分类号： C12N15/82 ,  C07K14/325 ,  A01N63/02 ,  A01N37/46

摘要： The subject invention concerns Bacillus thuringiensis modified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

公开(公告)号：US20150366211A1

公开(公告)日：2015-12-24

申请号：US14740326

申请日：2015-06-16

申请人： Dow AgroSciences LLC

发明人： Janna Mai Armstrong ,  Audrey Jane Etter ,  Meghan L. F. Frey ,  Premchand Gandra ,  Ted Letherer ,  Gaofeng Lin ,  Krishna M. Madduri ,  Haley R. Mowery ,  Kenneth Narva ,  Joel J. Sheets ,  Sek Yee Tan

IPC分类号： A01N47/44 ,  A01H1/02 ,  C07K14/32 ,  C12N15/82

CPC分类号： C07K14/32 ,  A01N37/46 ,  A01N63/02 ,  C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： DIG-657 vegetative insecticidal toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed. The invention includes DIG-657 variants, fragments and analogs.

摘要翻译： 公开了DIG-657营养性杀虫毒素，编码这种毒素的多核苷酸，使用这些毒素来控制害虫，以及产生这种毒素的转基因植物。 本发明包括DIG-657变体，片段和类似物。