公开(公告)号：US20140173780A1

公开(公告)日：2014-06-19

申请号：US14133370

申请日：2013-12-18

Applicant: DOW AGROSCIENCES LLC

Inventor： Dayakar Pareddy ,  Sivarama R. Chennareddy ,  Tatyana Minnicks ,  Olga Karpova ,  David Griffin ,  Jayakumar P. Samuel ,  Kelley A. Smith ,  Rodrigo Sarria-Millan ,  Tejinder Kumar Mall

IPC: C12N15/82

CPC classification number: C12N15/8286 ,  A01H5/00 ,  C12N15/8205 ,  C12N15/8245 ,  C12N15/8247 ,  C12N15/8274 ,  C12N15/8281 ,  C12N15/8283

Abstract: A method is disclosed for the Agrobacterium-mediated germline transformation of soybean, comprising infecting split soybean seeds, with a portion of the embryonic axis, with Agrobacterium tumefaciens containing a transgene. The method can further comprise regenerating the explants produced from the transformation of the split soybean seeds comprising a portion of embryonic axis in vitro on selection medium.

公开(公告)号：US20180110196A1

公开(公告)日：2018-04-26

申请号：US15786916

申请日：2017-10-18

Applicant: Dow AgroSciences LLC

Inventor： Sivarama Reddy Chennareddy ,  Dayakar Pareddy ,  Tobias M. (Toby) Cicak ,  Rodrigo Sarria ,  Katherine K. Effinger ,  Tejinder Kumar Mall

IPC: A01H4/00 ,  A01H5/10 ,  C12N15/82 ,  C12N5/00 ,  C07K14/425

CPC classification number: A01H4/005 ,  A01H4/00 ,  A01H4/008 ,  A01H5/10 ,  C07K14/425 ,  C12N5/0025 ,  C12N15/8205 ,  C12N15/8206 ,  C12N15/8234

Abstract: The invention provides a method for the production of soybean embryogenic callus and somatic embryos. The invention further provides methods of transforming explants that includes generating somatic embryo tissue. The transgenic somatic embryos produced can be used for regenerating stable transgenic plants.